Experiment: LB 0.5x with Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yoaE and manX are separated by 462 nucleotides manX and manY are separated by 62 nucleotides manY and manZ are separated by 12 nucleotides
b1816: yoaE - fused predicted membrane protein/conserved protein (NCBI), at 1,898,053 to 1,899,609
yoaE
b1817: manX - fused mannose-specific PTS enzymes: IIA component/IIB component (NCBI), at 1,900,072 to 1,901,043
manX
b1818: manY - mannose-specific enzyme IIC component of PTS (NCBI), at 1,901,106 to 1,901,906
manY
b1819: manZ - mannose-specific enzyme IID component of PTS (NCBI), at 1,901,919 to 1,902,770
manZ
Position (kb)
1900
1901
1902 Strain fitness (log2 ratio)
-1
0
1 at 1899.103 kb on + strand, within yoaE at 1899.103 kb on + strand, within yoaE at 1899.429 kb on + strand, within yoaE at 1899.689 kb on + strand at 1899.769 kb on + strand at 1899.804 kb on + strand at 1899.991 kb on - strand at 1900.072 kb on - strand at 1900.112 kb on - strand at 1900.117 kb on - strand at 1900.225 kb on + strand, within manX at 1900.253 kb on - strand, within manX at 1900.413 kb on + strand, within manX at 1900.611 kb on + strand, within manX at 1900.651 kb on - strand, within manX at 1900.702 kb on + strand, within manX at 1900.730 kb on + strand, within manX at 1900.787 kb on + strand, within manX at 1900.864 kb on - strand, within manX at 1900.885 kb on - strand, within manX at 1900.885 kb on - strand, within manX at 1900.887 kb on + strand, within manX at 1900.887 kb on + strand, within manX at 1900.964 kb on + strand at 1900.972 kb on - strand at 1901.109 kb on - strand at 1901.109 kb on - strand at 1901.147 kb on + strand at 1901.150 kb on - strand at 1901.150 kb on - strand at 1901.406 kb on + strand, within manY at 1901.560 kb on - strand, within manY at 1901.794 kb on - strand, within manY
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 1,899,103 + yoaE b1816 0.67 -1.0 1,899,103 + yoaE b1816 0.67 -0.2 1,899,429 + yoaE b1816 0.88 -0.5 1,899,689 + +1.2 1,899,769 + +0.5 1,899,804 + -0.1 1,899,991 - +1.0 1,900,072 - +0.2 1,900,112 - -0.0 1,900,117 - -0.0 1,900,225 + manX b1817 0.16 -0.4 1,900,253 - manX b1817 0.19 -0.4 1,900,413 + manX b1817 0.35 +0.0 1,900,611 + manX b1817 0.55 -0.2 1,900,651 - manX b1817 0.60 +0.5 1,900,702 + manX b1817 0.65 -0.2 1,900,730 + manX b1817 0.68 -0.3 1,900,787 + manX b1817 0.74 +0.1 1,900,864 - manX b1817 0.81 -0.1 1,900,885 - manX b1817 0.84 +0.4 1,900,885 - manX b1817 0.84 +0.3 1,900,887 + manX b1817 0.84 -0.9 1,900,887 + manX b1817 0.84 +0.1 1,900,964 + +0.5 1,900,972 - -0.0 1,901,109 - -0.4 1,901,109 - +0.7 1,901,147 + +0.2 1,901,150 - +0.2 1,901,150 - +0.1 1,901,406 + manY b1818 0.37 +0.9 1,901,560 - manY b1818 0.57 +0.0 1,901,794 - manY b1818 0.86 +0.2
Or see this region's nucleotide sequence