Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt astB and astD overlap by 4 nucleotides astD and astA overlap by 4 nucleotides
b1745: astB - succinylarginine dihydrolase (NCBI), at 1,824,940 to 1,826,283
astB
b1746: astD - succinylglutamic semialdehyde dehydrogenase (NCBI), at 1,826,280 to 1,827,758
astD
b1747: astA - arginine succinyltransferase (NCBI), at 1,827,755 to 1,828,789
astA
Position (kb)
1826
1827
1828 Strain fitness (log2 ratio)
-1
0
1 at 1825.285 kb on + strand, within astB at 1825.285 kb on + strand, within astB at 1825.293 kb on - strand, within astB at 1825.321 kb on - strand, within astB at 1825.321 kb on - strand, within astB at 1825.378 kb on + strand, within astB at 1825.727 kb on - strand, within astB at 1825.767 kb on - strand, within astB at 1826.034 kb on + strand, within astB at 1826.113 kb on - strand, within astB at 1826.358 kb on + strand at 1826.451 kb on - strand, within astD at 1826.451 kb on - strand, within astD at 1826.451 kb on - strand, within astD at 1826.614 kb on + strand, within astD at 1826.707 kb on + strand, within astD at 1826.730 kb on - strand, within astD at 1826.730 kb on - strand, within astD at 1826.786 kb on + strand, within astD at 1826.848 kb on - strand, within astD at 1826.848 kb on - strand, within astD at 1826.870 kb on - strand, within astD at 1826.949 kb on - strand, within astD at 1826.949 kb on - strand, within astD at 1827.115 kb on + strand, within astD at 1827.262 kb on - strand, within astD at 1827.262 kb on - strand, within astD at 1827.350 kb on - strand, within astD at 1827.814 kb on + strand at 1827.814 kb on + strand at 1827.870 kb on - strand, within astA at 1827.875 kb on + strand, within astA at 1828.010 kb on - strand, within astA at 1828.010 kb on - strand, within astA at 1828.024 kb on - strand, within astA at 1828.076 kb on - strand, within astA at 1828.076 kb on - strand, within astA at 1828.129 kb on - strand, within astA at 1828.236 kb on + strand, within astA at 1828.424 kb on - strand, within astA at 1828.629 kb on + strand, within astA
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 1,825,285 + astB b1745 0.26 +0.2 1,825,285 + astB b1745 0.26 -0.3 1,825,293 - astB b1745 0.26 -0.3 1,825,321 - astB b1745 0.28 +0.1 1,825,321 - astB b1745 0.28 +0.3 1,825,378 + astB b1745 0.33 -0.2 1,825,727 - astB b1745 0.59 +0.1 1,825,767 - astB b1745 0.62 -0.3 1,826,034 + astB b1745 0.81 +0.6 1,826,113 - astB b1745 0.87 -0.4 1,826,358 + -0.7 1,826,451 - astD b1746 0.12 -0.2 1,826,451 - astD b1746 0.12 +0.5 1,826,451 - astD b1746 0.12 -1.2 1,826,614 + astD b1746 0.23 -0.3 1,826,707 + astD b1746 0.29 -0.7 1,826,730 - astD b1746 0.30 -0.0 1,826,730 - astD b1746 0.30 -0.0 1,826,786 + astD b1746 0.34 +0.5 1,826,848 - astD b1746 0.38 +0.0 1,826,848 - astD b1746 0.38 +0.2 1,826,870 - astD b1746 0.40 +0.3 1,826,949 - astD b1746 0.45 -0.6 1,826,949 - astD b1746 0.45 -1.0 1,827,115 + astD b1746 0.56 -0.4 1,827,262 - astD b1746 0.66 +0.4 1,827,262 - astD b1746 0.66 +0.0 1,827,350 - astD b1746 0.72 -0.4 1,827,814 + +0.8 1,827,814 + +0.8 1,827,870 - astA b1747 0.11 -0.2 1,827,875 + astA b1747 0.12 +0.1 1,828,010 - astA b1747 0.25 +0.5 1,828,010 - astA b1747 0.25 +0.1 1,828,024 - astA b1747 0.26 +0.1 1,828,076 - astA b1747 0.31 -0.4 1,828,076 - astA b1747 0.31 +0.3 1,828,129 - astA b1747 0.36 -0.3 1,828,236 + astA b1747 0.46 -0.6 1,828,424 - astA b1747 0.65 +0.0 1,828,629 + astA b1747 0.84 -0.6
Or see this region's nucleotide sequence