Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt endA and yggJ are separated by 79 nucleotides yggJ and gshB are separated by 12 nucleotides gshB and yqgE are separated by 108 nucleotides yqgE and yqgF overlap by 1 nucleotides
b2945: endA - DNA-specific endonuclease I (NCBI), at 3,088,369 to 3,089,076
endA
b2946: yggJ - orf, hypothetical protein (VIMSS), at 3,089,156 to 3,089,887
yggJ
b2947: gshB - glutathione synthetase (NCBI), at 3,089,900 to 3,090,850
gshB
b2948: yqgE - orf, hypothetical protein (VIMSS), at 3,090,959 to 3,091,522
yqgE
b2949: yqgF - Holliday junction resolvase-like protein (NCBI), at 3,091,522 to 3,091,938
yqgF
Position (kb)
3089
3090
3091 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3088.962 kb on + strand, within endA at 3088.962 kb on + strand, within endA at 3088.962 kb on + strand, within endA at 3088.970 kb on - strand, within endA at 3088.970 kb on - strand, within endA at 3089.020 kb on - strand at 3089.038 kb on - strand at 3089.038 kb on - strand at 3089.038 kb on - strand at 3089.191 kb on + strand at 3089.281 kb on - strand, within yggJ at 3089.295 kb on - strand, within yggJ at 3089.411 kb on + strand, within yggJ at 3089.476 kb on - strand, within yggJ at 3089.476 kb on - strand, within yggJ at 3089.586 kb on - strand, within yggJ at 3089.586 kb on - strand, within yggJ at 3089.634 kb on + strand, within yggJ at 3089.634 kb on + strand, within yggJ at 3089.642 kb on - strand, within yggJ at 3089.642 kb on - strand, within yggJ at 3089.895 kb on + strand at 3089.924 kb on - strand at 3090.004 kb on + strand, within gshB at 3090.085 kb on - strand, within gshB at 3090.085 kb on - strand, within gshB at 3090.092 kb on - strand, within gshB at 3090.222 kb on + strand, within gshB at 3090.458 kb on - strand, within gshB at 3090.479 kb on - strand, within gshB at 3090.479 kb on - strand, within gshB at 3090.486 kb on - strand, within gshB at 3090.562 kb on - strand, within gshB at 3090.562 kb on - strand, within gshB at 3090.576 kb on + strand, within gshB at 3090.811 kb on + strand at 3090.847 kb on - strand at 3090.847 kb on - strand at 3090.883 kb on + strand at 3090.883 kb on + strand at 3090.893 kb on - strand at 3090.893 kb on - strand at 3091.078 kb on - strand, within yqgE at 3091.078 kb on - strand, within yqgE at 3091.140 kb on + strand, within yqgE at 3091.140 kb on + strand, within yqgE at 3091.271 kb on - strand, within yqgE at 3091.271 kb on - strand, within yqgE at 3091.328 kb on + strand, within yqgE at 3091.534 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 3,088,962 + endA b2945 0.84 -0.1 3,088,962 + endA b2945 0.84 -0.2 3,088,962 + endA b2945 0.84 -0.3 3,088,970 - endA b2945 0.85 -0.0 3,088,970 - endA b2945 0.85 -0.2 3,089,020 - +0.3 3,089,038 - +0.0 3,089,038 - -0.4 3,089,038 - +0.8 3,089,191 + -0.1 3,089,281 - yggJ b2946 0.17 -2.5 3,089,295 - yggJ b2946 0.19 -0.3 3,089,411 + yggJ b2946 0.35 -5.7 3,089,476 - yggJ b2946 0.44 -0.2 3,089,476 - yggJ b2946 0.44 +0.4 3,089,586 - yggJ b2946 0.59 -1.3 3,089,586 - yggJ b2946 0.59 +0.2 3,089,634 + yggJ b2946 0.65 +0.3 3,089,634 + yggJ b2946 0.65 -0.2 3,089,642 - yggJ b2946 0.66 +0.6 3,089,642 - yggJ b2946 0.66 +0.2 3,089,895 + +0.1 3,089,924 - -0.2 3,090,004 + gshB b2947 0.11 -0.1 3,090,085 - gshB b2947 0.19 -1.0 3,090,085 - gshB b2947 0.19 +0.3 3,090,092 - gshB b2947 0.20 +0.3 3,090,222 + gshB b2947 0.34 -0.3 3,090,458 - gshB b2947 0.59 -0.4 3,090,479 - gshB b2947 0.61 +0.2 3,090,479 - gshB b2947 0.61 -0.2 3,090,486 - gshB b2947 0.62 -0.7 3,090,562 - gshB b2947 0.70 -0.6 3,090,562 - gshB b2947 0.70 +0.2 3,090,576 + gshB b2947 0.71 -0.4 3,090,811 + -0.1 3,090,847 - +0.3 3,090,847 - +0.6 3,090,883 + -0.7 3,090,883 + -0.2 3,090,893 - +0.0 3,090,893 - +0.2 3,091,078 - yqgE b2948 0.21 +0.1 3,091,078 - yqgE b2948 0.21 +0.3 3,091,140 + yqgE b2948 0.32 -0.2 3,091,140 + yqgE b2948 0.32 -0.1 3,091,271 - yqgE b2948 0.55 +1.0 3,091,271 - yqgE b2948 0.55 -0.6 3,091,328 + yqgE b2948 0.65 +0.4 3,091,534 + +0.1
Or see this region's nucleotide sequence