Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt galR and lysA are separated by 6 nucleotides lysA and lysR are separated by 121 nucleotides
b2837: galR - DNA-binding transcriptional repressor (NCBI), at 2,974,621 to 2,975,652
galR
b2838: lysA - diaminopimelate decarboxylase, PLP-binding (NCBI), at 2,975,659 to 2,976,921
lysA
b2839: lysR - DNA-binding transcriptional dual regulator (NCBI), at 2,977,043 to 2,977,978
lysR
Position (kb)
2975
2976
2977 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2974.684 kb on + strand at 2974.684 kb on + strand at 2974.723 kb on + strand at 2974.733 kb on + strand, within galR at 2974.733 kb on + strand, within galR at 2974.741 kb on - strand, within galR at 2974.741 kb on - strand, within galR at 2974.751 kb on + strand, within galR at 2974.793 kb on + strand, within galR at 2974.793 kb on + strand, within galR at 2975.062 kb on - strand, within galR at 2975.089 kb on + strand, within galR at 2975.111 kb on - strand, within galR at 2975.111 kb on - strand, within galR at 2975.152 kb on - strand, within galR at 2975.168 kb on + strand, within galR at 2975.176 kb on - strand, within galR at 2975.348 kb on + strand, within galR at 2975.354 kb on - strand, within galR at 2975.357 kb on + strand, within galR at 2975.399 kb on + strand, within galR at 2975.399 kb on + strand, within galR at 2975.406 kb on - strand, within galR at 2975.406 kb on - strand, within galR at 2975.407 kb on - strand, within galR at 2975.457 kb on + strand, within galR at 2975.457 kb on + strand, within galR at 2975.478 kb on - strand, within galR at 2975.516 kb on - strand, within galR at 2975.627 kb on - strand at 2975.794 kb on - strand, within lysA at 2975.794 kb on - strand, within lysA at 2975.806 kb on - strand, within lysA at 2975.816 kb on - strand, within lysA at 2975.871 kb on + strand, within lysA at 2975.883 kb on - strand, within lysA at 2975.917 kb on + strand, within lysA at 2975.984 kb on + strand, within lysA at 2976.036 kb on - strand, within lysA at 2976.036 kb on - strand, within lysA at 2976.041 kb on - strand, within lysA at 2976.216 kb on - strand, within lysA at 2976.302 kb on + strand, within lysA at 2976.305 kb on + strand, within lysA at 2976.305 kb on + strand, within lysA at 2976.305 kb on - strand, within lysA at 2976.305 kb on - strand, within lysA at 2976.313 kb on - strand, within lysA at 2976.319 kb on - strand, within lysA at 2976.345 kb on - strand, within lysA at 2976.397 kb on - strand, within lysA at 2976.401 kb on + strand, within lysA at 2976.420 kb on + strand, within lysA at 2976.424 kb on - strand, within lysA at 2976.424 kb on - strand, within lysA at 2976.428 kb on - strand, within lysA at 2976.428 kb on - strand, within lysA at 2976.479 kb on - strand, within lysA at 2976.494 kb on + strand, within lysA at 2976.501 kb on + strand, within lysA at 2976.501 kb on + strand, within lysA at 2976.532 kb on - strand, within lysA at 2976.532 kb on - strand, within lysA at 2976.554 kb on - strand, within lysA at 2976.681 kb on + strand, within lysA at 2976.681 kb on + strand, within lysA at 2976.827 kb on + strand at 2976.827 kb on + strand at 2976.835 kb on - strand at 2976.835 kb on - strand at 2976.899 kb on + strand at 2976.907 kb on - strand at 2976.926 kb on - strand at 2977.048 kb on - strand at 2977.235 kb on - strand, within lysR at 2977.253 kb on + strand, within lysR at 2977.339 kb on - strand, within lysR at 2977.339 kb on - strand, within lysR at 2977.671 kb on - strand, within lysR at 2977.719 kb on + strand, within lysR at 2977.792 kb on - strand, within lysR at 2977.830 kb on + strand, within lysR at 2977.830 kb on - strand, within lysR at 2977.838 kb on + strand, within lysR at 2977.838 kb on + strand, within lysR at 2977.888 kb on + strand at 2977.891 kb on - strand at 2977.891 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 2,974,684 + -0.5 2,974,684 + +0.1 2,974,723 + -0.3 2,974,733 + galR b2837 0.11 -0.3 2,974,733 + galR b2837 0.11 +0.5 2,974,741 - galR b2837 0.12 -0.5 2,974,741 - galR b2837 0.12 +0.0 2,974,751 + galR b2837 0.13 -0.1 2,974,793 + galR b2837 0.17 -0.4 2,974,793 + galR b2837 0.17 +0.3 2,975,062 - galR b2837 0.43 -0.2 2,975,089 + galR b2837 0.45 +0.3 2,975,111 - galR b2837 0.47 -0.3 2,975,111 - galR b2837 0.47 -0.3 2,975,152 - galR b2837 0.51 +0.8 2,975,168 + galR b2837 0.53 +0.2 2,975,176 - galR b2837 0.54 +0.1 2,975,348 + galR b2837 0.70 +0.5 2,975,354 - galR b2837 0.71 -0.1 2,975,357 + galR b2837 0.71 +0.3 2,975,399 + galR b2837 0.75 +0.4 2,975,399 + galR b2837 0.75 +0.1 2,975,406 - galR b2837 0.76 +0.1 2,975,406 - galR b2837 0.76 +0.7 2,975,407 - galR b2837 0.76 -0.5 2,975,457 + galR b2837 0.81 +0.4 2,975,457 + galR b2837 0.81 -0.1 2,975,478 - galR b2837 0.83 -0.2 2,975,516 - galR b2837 0.87 -0.0 2,975,627 - -0.9 2,975,794 - lysA b2838 0.11 -4.0 2,975,794 - lysA b2838 0.11 -3.0 2,975,806 - lysA b2838 0.12 -2.9 2,975,816 - lysA b2838 0.12 -2.1 2,975,871 + lysA b2838 0.17 -3.0 2,975,883 - lysA b2838 0.18 -1.1 2,975,917 + lysA b2838 0.20 -4.2 2,975,984 + lysA b2838 0.26 -5.4 2,976,036 - lysA b2838 0.30 -2.7 2,976,036 - lysA b2838 0.30 -1.9 2,976,041 - lysA b2838 0.30 -3.9 2,976,216 - lysA b2838 0.44 -4.6 2,976,302 + lysA b2838 0.51 -2.2 2,976,305 + lysA b2838 0.51 -1.6 2,976,305 + lysA b2838 0.51 -3.3 2,976,305 - lysA b2838 0.51 -3.7 2,976,305 - lysA b2838 0.51 -5.5 2,976,313 - lysA b2838 0.52 -3.3 2,976,319 - lysA b2838 0.52 -2.8 2,976,345 - lysA b2838 0.54 -1.9 2,976,397 - lysA b2838 0.58 -3.7 2,976,401 + lysA b2838 0.59 -2.7 2,976,420 + lysA b2838 0.60 -4.2 2,976,424 - lysA b2838 0.61 -2.8 2,976,424 - lysA b2838 0.61 -4.5 2,976,428 - lysA b2838 0.61 -2.4 2,976,428 - lysA b2838 0.61 -3.7 2,976,479 - lysA b2838 0.65 -3.3 2,976,494 + lysA b2838 0.66 -4.3 2,976,501 + lysA b2838 0.67 -3.6 2,976,501 + lysA b2838 0.67 -4.3 2,976,532 - lysA b2838 0.69 -4.2 2,976,532 - lysA b2838 0.69 -2.2 2,976,554 - lysA b2838 0.71 -2.7 2,976,681 + lysA b2838 0.81 -1.8 2,976,681 + lysA b2838 0.81 -3.9 2,976,827 + -3.0 2,976,827 + -2.3 2,976,835 - -1.3 2,976,835 - -5.9 2,976,899 + -3.4 2,976,907 - -2.5 2,976,926 - +0.1 2,977,048 - +0.1 2,977,235 - lysR b2839 0.21 -1.5 2,977,253 + lysR b2839 0.22 -2.9 2,977,339 - lysR b2839 0.32 -3.4 2,977,339 - lysR b2839 0.32 -1.9 2,977,671 - lysR b2839 0.67 -5.0 2,977,719 + lysR b2839 0.72 -3.9 2,977,792 - lysR b2839 0.80 -2.3 2,977,830 + lysR b2839 0.84 -3.7 2,977,830 - lysR b2839 0.84 -4.4 2,977,838 + lysR b2839 0.85 -4.9 2,977,838 + lysR b2839 0.85 -3.0 2,977,888 + -2.5 2,977,891 - -3.3 2,977,891 - -2.2
Or see this region's nucleotide sequence