Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt norV and norW overlap by 4 nucleotides norW and hypF are separated by 127 nucleotides
b2710: norV - anaerobic nitric oxide reductase flavorubredoxin (NCBI), at 2,830,498 to 2,831,937
norV
b2711: norW - nitric oxide reductase (NCBI), at 2,831,934 to 2,833,067
norW
b2712: hypF - carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases (NCBI), at 2,833,195 to 2,835,447
hypF
Position (kb)
2831
2832
2833
2834 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2830.983 kb on + strand, within norV at 2830.986 kb on - strand, within norV at 2830.986 kb on - strand, within norV at 2831.011 kb on - strand, within norV at 2831.011 kb on - strand, within norV at 2831.018 kb on - strand, within norV at 2831.018 kb on - strand, within norV at 2831.075 kb on - strand, within norV at 2831.075 kb on - strand, within norV at 2831.092 kb on - strand, within norV at 2831.109 kb on + strand, within norV at 2831.134 kb on + strand, within norV at 2831.273 kb on + strand, within norV at 2831.273 kb on + strand, within norV at 2831.314 kb on + strand, within norV at 2831.314 kb on + strand, within norV at 2831.629 kb on + strand, within norV at 2831.646 kb on - strand, within norV at 2831.646 kb on - strand, within norV at 2831.651 kb on + strand, within norV at 2831.706 kb on - strand, within norV at 2831.732 kb on - strand, within norV at 2831.748 kb on + strand, within norV at 2831.748 kb on + strand, within norV at 2831.829 kb on + strand at 2831.934 kb on - strand at 2831.957 kb on - strand at 2832.048 kb on + strand, within norW at 2832.064 kb on - strand, within norW at 2832.150 kb on - strand, within norW at 2832.281 kb on + strand, within norW at 2832.281 kb on + strand, within norW at 2832.364 kb on + strand, within norW at 2832.384 kb on - strand, within norW at 2832.384 kb on - strand, within norW at 2832.526 kb on + strand, within norW at 2832.896 kb on - strand, within norW at 2832.956 kb on + strand at 2833.005 kb on + strand at 2833.005 kb on + strand at 2833.046 kb on + strand at 2833.054 kb on - strand at 2833.119 kb on + strand at 2833.119 kb on + strand at 2833.350 kb on + strand at 2833.350 kb on + strand at 2833.353 kb on - strand at 2833.371 kb on + strand at 2833.399 kb on - strand at 2833.399 kb on - strand at 2833.408 kb on + strand at 2833.423 kb on + strand, within hypF at 2833.423 kb on + strand, within hypF at 2833.581 kb on + strand, within hypF at 2833.699 kb on + strand, within hypF at 2833.734 kb on + strand, within hypF at 2833.934 kb on - strand, within hypF at 2833.962 kb on + strand, within hypF at 2833.962 kb on + strand, within hypF at 2833.980 kb on - strand, within hypF
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 2,830,983 + norV b2710 0.34 -0.3 2,830,986 - norV b2710 0.34 -0.1 2,830,986 - norV b2710 0.34 -0.5 2,831,011 - norV b2710 0.36 -1.3 2,831,011 - norV b2710 0.36 -0.1 2,831,018 - norV b2710 0.36 +0.4 2,831,018 - norV b2710 0.36 -0.4 2,831,075 - norV b2710 0.40 -0.5 2,831,075 - norV b2710 0.40 -0.0 2,831,092 - norV b2710 0.41 +0.9 2,831,109 + norV b2710 0.42 -0.0 2,831,134 + norV b2710 0.44 -0.2 2,831,273 + norV b2710 0.54 +0.3 2,831,273 + norV b2710 0.54 -0.5 2,831,314 + norV b2710 0.57 -0.9 2,831,314 + norV b2710 0.57 -0.1 2,831,629 + norV b2710 0.79 -0.4 2,831,646 - norV b2710 0.80 +0.1 2,831,646 - norV b2710 0.80 -0.1 2,831,651 + norV b2710 0.80 -0.1 2,831,706 - norV b2710 0.84 +0.0 2,831,732 - norV b2710 0.86 -0.8 2,831,748 + norV b2710 0.87 +0.0 2,831,748 + norV b2710 0.87 +0.1 2,831,829 + -0.1 2,831,934 - -0.3 2,831,957 - +0.3 2,832,048 + norW b2711 0.10 -0.0 2,832,064 - norW b2711 0.11 +0.0 2,832,150 - norW b2711 0.19 -0.2 2,832,281 + norW b2711 0.31 -0.1 2,832,281 + norW b2711 0.31 -0.0 2,832,364 + norW b2711 0.38 +0.1 2,832,384 - norW b2711 0.40 -0.4 2,832,384 - norW b2711 0.40 +0.2 2,832,526 + norW b2711 0.52 -0.4 2,832,896 - norW b2711 0.85 -0.4 2,832,956 + -0.2 2,833,005 + +0.7 2,833,005 + -0.3 2,833,046 + -0.3 2,833,054 - -0.1 2,833,119 + -0.1 2,833,119 + -0.5 2,833,350 + -0.0 2,833,350 + +0.2 2,833,353 - -3.0 2,833,371 + -0.5 2,833,399 - -0.2 2,833,399 - -0.5 2,833,408 + +0.3 2,833,423 + hypF b2712 0.10 -0.7 2,833,423 + hypF b2712 0.10 -0.8 2,833,581 + hypF b2712 0.17 -0.3 2,833,699 + hypF b2712 0.22 +0.2 2,833,734 + hypF b2712 0.24 -0.1 2,833,934 - hypF b2712 0.33 -0.4 2,833,962 + hypF b2712 0.34 +0.2 2,833,962 + hypF b2712 0.34 -0.1 2,833,980 - hypF b2712 0.35 -0.2
Or see this region's nucleotide sequence