Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tyrA and aroF are separated by 10 nucleotides aroF and yfiL are separated by 209 nucleotides yfiL and yfiR are separated by 149 nucleotides
b2600: tyrA - fused chorismate mutase T/prephenate dehydrogenase (NCBI), at 2,736,970 to 2,738,091
tyrA
b2601: aroF - 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, tyrosine-repressible (NCBI), at 2,738,102 to 2,739,172
aroF
b2602: yfiL - orf, hypothetical protein (VIMSS), at 2,739,382 to 2,739,747
yfiL
b2603: yfiR - hypothetical protein (NCBI), at 2,739,897 to 2,740,415
yfiR
Position (kb)
2738
2739
2740 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2737.175 kb on - strand, within tyrA at 2737.294 kb on + strand, within tyrA at 2737.399 kb on - strand, within tyrA at 2737.589 kb on - strand, within tyrA at 2737.698 kb on - strand, within tyrA at 2737.698 kb on - strand, within tyrA at 2737.712 kb on - strand, within tyrA at 2737.753 kb on - strand, within tyrA at 2737.753 kb on - strand, within tyrA at 2737.877 kb on - strand, within tyrA at 2737.877 kb on - strand, within tyrA at 2737.890 kb on - strand, within tyrA at 2737.978 kb on + strand, within tyrA at 2737.978 kb on + strand, within tyrA at 2738.079 kb on + strand at 2738.079 kb on + strand at 2738.079 kb on + strand at 2738.099 kb on - strand at 2738.201 kb on - strand at 2738.366 kb on - strand, within aroF at 2738.378 kb on - strand, within aroF at 2738.445 kb on + strand, within aroF at 2738.534 kb on - strand, within aroF at 2738.534 kb on - strand, within aroF at 2738.714 kb on + strand, within aroF at 2738.714 kb on + strand, within aroF at 2738.768 kb on - strand, within aroF at 2738.768 kb on - strand, within aroF at 2738.829 kb on + strand, within aroF at 2738.829 kb on + strand, within aroF at 2738.897 kb on + strand, within aroF at 2738.899 kb on + strand, within aroF at 2738.905 kb on + strand, within aroF at 2738.926 kb on + strand, within aroF at 2738.926 kb on + strand, within aroF at 2738.945 kb on - strand, within aroF at 2738.999 kb on - strand, within aroF at 2738.999 kb on - strand, within aroF at 2739.001 kb on + strand, within aroF at 2739.008 kb on - strand, within aroF at 2739.008 kb on - strand, within aroF at 2739.420 kb on + strand, within yfiL at 2739.431 kb on + strand, within yfiL at 2739.431 kb on + strand, within yfiL at 2739.470 kb on + strand, within yfiL at 2739.473 kb on - strand, within yfiL at 2739.478 kb on - strand, within yfiL at 2739.478 kb on - strand, within yfiL at 2739.479 kb on + strand, within yfiL at 2739.490 kb on + strand, within yfiL at 2739.492 kb on - strand, within yfiL at 2739.499 kb on - strand, within yfiL at 2739.499 kb on - strand, within yfiL at 2739.545 kb on + strand, within yfiL at 2739.545 kb on + strand, within yfiL at 2739.571 kb on + strand, within yfiL at 2739.571 kb on - strand, within yfiL at 2739.571 kb on - strand, within yfiL at 2739.575 kb on - strand, within yfiL at 2739.575 kb on - strand, within yfiL at 2739.577 kb on + strand, within yfiL at 2739.578 kb on - strand, within yfiL at 2739.578 kb on - strand, within yfiL at 2739.588 kb on - strand, within yfiL at 2739.650 kb on + strand, within yfiL at 2739.676 kb on - strand, within yfiL at 2739.676 kb on - strand, within yfiL at 2739.677 kb on + strand, within yfiL at 2739.677 kb on + strand, within yfiL at 2739.697 kb on + strand, within yfiL at 2739.697 kb on + strand, within yfiL at 2739.705 kb on - strand, within yfiL at 2739.817 kb on - strand at 2739.914 kb on + strand at 2739.914 kb on + strand at 2739.951 kb on - strand, within yfiR at 2739.959 kb on + strand, within yfiR at 2739.963 kb on - strand, within yfiR at 2739.967 kb on - strand, within yfiR at 2739.969 kb on - strand, within yfiR at 2739.970 kb on + strand, within yfiR at 2739.970 kb on - strand, within yfiR at 2740.012 kb on - strand, within yfiR at 2740.021 kb on - strand, within yfiR at 2740.081 kb on + strand, within yfiR at 2740.090 kb on + strand, within yfiR at 2740.092 kb on + strand, within yfiR at 2740.092 kb on + strand, within yfiR at 2740.127 kb on + strand, within yfiR at 2740.127 kb on + strand, within yfiR at 2740.127 kb on + strand, within yfiR at 2740.146 kb on + strand, within yfiR at 2740.146 kb on + strand, within yfiR at 2740.146 kb on + strand, within yfiR at 2740.154 kb on - strand, within yfiR
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 2,737,175 - tyrA b2600 0.18 -3.7 2,737,294 + tyrA b2600 0.29 -2.9 2,737,399 - tyrA b2600 0.38 -2.2 2,737,589 - tyrA b2600 0.55 -3.1 2,737,698 - tyrA b2600 0.65 -3.4 2,737,698 - tyrA b2600 0.65 -3.3 2,737,712 - tyrA b2600 0.66 -3.1 2,737,753 - tyrA b2600 0.70 -2.2 2,737,753 - tyrA b2600 0.70 -3.0 2,737,877 - tyrA b2600 0.81 -1.9 2,737,877 - tyrA b2600 0.81 -2.7 2,737,890 - tyrA b2600 0.82 -0.3 2,737,978 + tyrA b2600 0.90 -1.3 2,737,978 + tyrA b2600 0.90 -2.4 2,738,079 + -2.8 2,738,079 + -4.2 2,738,079 + -2.8 2,738,099 - +0.5 2,738,201 - -0.9 2,738,366 - aroF b2601 0.25 -1.2 2,738,378 - aroF b2601 0.26 +0.7 2,738,445 + aroF b2601 0.32 -2.6 2,738,534 - aroF b2601 0.40 -0.3 2,738,534 - aroF b2601 0.40 -1.3 2,738,714 + aroF b2601 0.57 -2.1 2,738,714 + aroF b2601 0.57 -2.7 2,738,768 - aroF b2601 0.62 -0.3 2,738,768 - aroF b2601 0.62 -0.9 2,738,829 + aroF b2601 0.68 -2.3 2,738,829 + aroF b2601 0.68 -2.3 2,738,897 + aroF b2601 0.74 -2.1 2,738,899 + aroF b2601 0.74 -2.7 2,738,905 + aroF b2601 0.75 -1.9 2,738,926 + aroF b2601 0.77 -2.4 2,738,926 + aroF b2601 0.77 -3.5 2,738,945 - aroF b2601 0.79 -0.0 2,738,999 - aroF b2601 0.84 +0.1 2,738,999 - aroF b2601 0.84 -0.3 2,739,001 + aroF b2601 0.84 -2.0 2,739,008 - aroF b2601 0.85 -0.0 2,739,008 - aroF b2601 0.85 -3.2 2,739,420 + yfiL b2602 0.10 -0.7 2,739,431 + yfiL b2602 0.13 +1.0 2,739,431 + yfiL b2602 0.13 -0.4 2,739,470 + yfiL b2602 0.24 -0.0 2,739,473 - yfiL b2602 0.25 -0.1 2,739,478 - yfiL b2602 0.26 +0.8 2,739,478 - yfiL b2602 0.26 -0.1 2,739,479 + yfiL b2602 0.27 -0.2 2,739,490 + yfiL b2602 0.30 +1.0 2,739,492 - yfiL b2602 0.30 -0.7 2,739,499 - yfiL b2602 0.32 -0.6 2,739,499 - yfiL b2602 0.32 +1.0 2,739,545 + yfiL b2602 0.45 -0.2 2,739,545 + yfiL b2602 0.45 -0.2 2,739,571 + yfiL b2602 0.52 +0.3 2,739,571 - yfiL b2602 0.52 -1.2 2,739,571 - yfiL b2602 0.52 +0.6 2,739,575 - yfiL b2602 0.53 +0.8 2,739,575 - yfiL b2602 0.53 -0.4 2,739,577 + yfiL b2602 0.53 +0.2 2,739,578 - yfiL b2602 0.54 -1.2 2,739,578 - yfiL b2602 0.54 -0.2 2,739,588 - yfiL b2602 0.56 +0.1 2,739,650 + yfiL b2602 0.73 +0.0 2,739,676 - yfiL b2602 0.80 +0.7 2,739,676 - yfiL b2602 0.80 -0.3 2,739,677 + yfiL b2602 0.81 -0.1 2,739,677 + yfiL b2602 0.81 -0.7 2,739,697 + yfiL b2602 0.86 +0.0 2,739,697 + yfiL b2602 0.86 +0.0 2,739,705 - yfiL b2602 0.88 +0.4 2,739,817 - +0.1 2,739,914 + -0.2 2,739,914 + +0.2 2,739,951 - yfiR b2603 0.10 -0.2 2,739,959 + yfiR b2603 0.12 +0.2 2,739,963 - yfiR b2603 0.13 -0.6 2,739,967 - yfiR b2603 0.13 -0.0 2,739,969 - yfiR b2603 0.14 +0.5 2,739,970 + yfiR b2603 0.14 +1.0 2,739,970 - yfiR b2603 0.14 -0.5 2,740,012 - yfiR b2603 0.22 -2.2 2,740,021 - yfiR b2603 0.24 +0.5 2,740,081 + yfiR b2603 0.35 +0.6 2,740,090 + yfiR b2603 0.37 +0.0 2,740,092 + yfiR b2603 0.38 +0.1 2,740,092 + yfiR b2603 0.38 -0.1 2,740,127 + yfiR b2603 0.44 +0.9 2,740,127 + yfiR b2603 0.44 +0.1 2,740,127 + yfiR b2603 0.44 -0.2 2,740,146 + yfiR b2603 0.48 -0.2 2,740,146 + yfiR b2603 0.48 -0.4 2,740,146 + yfiR b2603 0.48 +0.2 2,740,154 - yfiR b2603 0.50 +0.3
Or see this region's nucleotide sequence