Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpoE and nadB are separated by 407 nucleotides nadB and yfiC overlap by 16 nucleotides yfiC and srmB are separated by 131 nucleotides
b2573: rpoE - RNA polymerase, sigma 24 (sigma E) factor (NCBI), at 2,707,459 to 2,708,034
rpoE
b2574: nadB - L-aspartate oxidase (NCBI), at 2,708,442 to 2,710,064
nadB
b2575: yfiC - putative enzyme (VIMSS), at 2,710,049 to 2,710,786
yfiC
b2576: srmB - ATP-dependent RNA helicase (NCBI), at 2,710,918 to 2,712,252
srmB
Position (kb)
2708
2709
2710
2711 Strain fitness (log2 ratio)
-2
-1
0
1 at 2708.179 kb on - strand at 2708.179 kb on - strand at 2708.218 kb on - strand at 2708.218 kb on - strand at 2708.221 kb on + strand at 2708.239 kb on - strand at 2708.289 kb on + strand at 2708.289 kb on + strand at 2708.309 kb on - strand at 2708.401 kb on - strand at 2708.406 kb on - strand at 2708.406 kb on - strand at 2708.411 kb on - strand at 2708.443 kb on + strand at 2708.443 kb on + strand at 2708.459 kb on + strand at 2708.492 kb on - strand at 2708.516 kb on + strand at 2708.524 kb on - strand at 2708.564 kb on - strand at 2708.564 kb on - strand at 2708.581 kb on + strand at 2708.627 kb on - strand, within nadB at 2708.650 kb on + strand, within nadB at 2708.688 kb on + strand, within nadB at 2708.721 kb on + strand, within nadB at 2708.721 kb on + strand, within nadB at 2708.766 kb on - strand, within nadB at 2708.797 kb on - strand, within nadB at 2708.858 kb on + strand at 2708.867 kb on - strand, within nadB at 2708.867 kb on - strand, within nadB at 2708.871 kb on + strand, within nadB at 2709.037 kb on - strand, within nadB at 2709.050 kb on + strand, within nadB at 2709.135 kb on + strand, within nadB at 2709.135 kb on + strand, within nadB at 2709.188 kb on - strand, within nadB at 2709.193 kb on - strand, within nadB at 2709.377 kb on - strand, within nadB at 2709.382 kb on + strand, within nadB at 2709.496 kb on + strand, within nadB at 2709.571 kb on + strand, within nadB at 2709.578 kb on - strand, within nadB at 2709.578 kb on - strand, within nadB at 2709.579 kb on + strand, within nadB at 2709.605 kb on + strand, within nadB at 2709.623 kb on - strand, within nadB at 2709.680 kb on - strand, within nadB at 2709.709 kb on - strand, within nadB at 2709.858 kb on - strand, within nadB at 2709.900 kb on - strand, within nadB at 2709.919 kb on + strand at 2709.942 kb on - strand at 2709.942 kb on - strand at 2709.949 kb on + strand at 2709.954 kb on + strand at 2709.957 kb on - strand at 2709.997 kb on + strand at 2710.005 kb on - strand at 2710.005 kb on - strand at 2710.005 kb on - strand at 2710.189 kb on - strand, within yfiC at 2710.236 kb on + strand, within yfiC at 2710.329 kb on + strand, within yfiC at 2710.375 kb on - strand, within yfiC at 2710.398 kb on + strand, within yfiC at 2710.399 kb on - strand, within yfiC at 2710.399 kb on - strand, within yfiC at 2710.402 kb on - strand, within yfiC at 2710.409 kb on + strand, within yfiC at 2710.410 kb on + strand, within yfiC at 2710.413 kb on - strand, within yfiC at 2710.413 kb on - strand, within yfiC at 2710.418 kb on - strand, within yfiC at 2710.418 kb on - strand, within yfiC at 2710.439 kb on - strand, within yfiC at 2710.439 kb on - strand, within yfiC at 2710.591 kb on - strand, within yfiC at 2710.770 kb on + strand at 2710.770 kb on + strand at 2710.770 kb on + strand at 2710.770 kb on + strand at 2710.778 kb on - strand at 2710.778 kb on - strand at 2710.833 kb on - strand at 2710.833 kb on - strand at 2710.838 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 2,708,179 - +0.5 2,708,179 - -0.4 2,708,218 - +0.3 2,708,218 - +0.5 2,708,221 + -0.9 2,708,239 - -0.4 2,708,289 + +0.5 2,708,289 + +0.1 2,708,309 - +0.3 2,708,401 - -0.1 2,708,406 - -0.0 2,708,406 - -0.1 2,708,411 - -1.8 2,708,443 + -0.2 2,708,443 + +0.2 2,708,459 + +0.4 2,708,492 - -0.3 2,708,516 + +0.1 2,708,524 - +0.7 2,708,564 - -0.4 2,708,564 - -0.5 2,708,581 + +0.5 2,708,627 - nadB b2574 0.11 -0.2 2,708,650 + nadB b2574 0.13 +0.2 2,708,688 + nadB b2574 0.15 +0.4 2,708,721 + nadB b2574 0.17 -0.5 2,708,721 + nadB b2574 0.17 -0.3 2,708,766 - nadB b2574 0.20 +0.1 2,708,797 - nadB b2574 0.22 -0.0 2,708,858 + -0.3 2,708,867 - nadB b2574 0.26 +0.5 2,708,867 - nadB b2574 0.26 -0.2 2,708,871 + nadB b2574 0.26 +0.1 2,709,037 - nadB b2574 0.37 +0.1 2,709,050 + nadB b2574 0.37 +1.5 2,709,135 + nadB b2574 0.43 -0.5 2,709,135 + nadB b2574 0.43 -0.5 2,709,188 - nadB b2574 0.46 -0.6 2,709,193 - nadB b2574 0.46 -0.5 2,709,377 - nadB b2574 0.58 +0.8 2,709,382 + nadB b2574 0.58 -1.3 2,709,496 + nadB b2574 0.65 -0.6 2,709,571 + nadB b2574 0.70 -0.7 2,709,578 - nadB b2574 0.70 -0.5 2,709,578 - nadB b2574 0.70 -0.5 2,709,579 + nadB b2574 0.70 -1.8 2,709,605 + nadB b2574 0.72 -0.3 2,709,623 - nadB b2574 0.73 -0.4 2,709,680 - nadB b2574 0.76 -0.4 2,709,709 - nadB b2574 0.78 +0.0 2,709,858 - nadB b2574 0.87 -0.3 2,709,900 - nadB b2574 0.90 -0.2 2,709,919 + -0.6 2,709,942 - -0.3 2,709,942 - +0.3 2,709,949 + +0.3 2,709,954 + -0.2 2,709,957 - +0.1 2,709,997 + -0.8 2,710,005 - +0.9 2,710,005 - +0.0 2,710,005 - +0.7 2,710,189 - yfiC b2575 0.19 +0.2 2,710,236 + yfiC b2575 0.25 +0.3 2,710,329 + yfiC b2575 0.38 +0.3 2,710,375 - yfiC b2575 0.44 -0.2 2,710,398 + yfiC b2575 0.47 +0.4 2,710,399 - yfiC b2575 0.47 +0.0 2,710,399 - yfiC b2575 0.47 +0.4 2,710,402 - yfiC b2575 0.48 -0.4 2,710,409 + yfiC b2575 0.49 +0.1 2,710,410 + yfiC b2575 0.49 -0.0 2,710,413 - yfiC b2575 0.49 +0.5 2,710,413 - yfiC b2575 0.49 -0.1 2,710,418 - yfiC b2575 0.50 +0.1 2,710,418 - yfiC b2575 0.50 -0.0 2,710,439 - yfiC b2575 0.53 +0.6 2,710,439 - yfiC b2575 0.53 +0.5 2,710,591 - yfiC b2575 0.73 +0.2 2,710,770 + -0.1 2,710,770 + -0.1 2,710,770 + -0.2 2,710,770 + -0.2 2,710,778 - -1.3 2,710,778 - -0.3 2,710,833 - +0.1 2,710,833 - -0.2 2,710,838 + -0.6
Or see this region's nucleotide sequence