Strain Fitness in Escherichia coli BW25113 around b2518

Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
2,641,671	+	yfgB	b2517	0.45	-0.7
2,641,688	+	yfgB	b2517	0.46	-1.2
2,641,748	+	yfgB	b2517	0.52	-0.3
2,641,748	+	yfgB	b2517	0.52	-0.9
2,641,784	-	yfgB	b2517	0.55	+0.3
2,641,819	+	yfgB	b2517	0.58	-0.1
2,641,819	+	yfgB	b2517	0.58	-0.4
2,641,923	-	yfgB	b2517	0.67	-0.1
2,641,947	+	yfgB	b2517	0.69	-0.4
2,641,947	+	yfgB	b2517	0.69	-0.6
2,642,153	-	yfgB	b2517	0.87	-0.6
2,642,153	-	yfgB	b2517	0.87	+0.4
2,642,304	-				-0.7
2,642,392	-				-0.5
2,642,392	-				+0.2
2,642,509	-	ndk	b2518	0.12	-2.5
2,642,509	-	ndk	b2518	0.12	-1.1
2,642,590	-	ndk	b2518	0.31	+0.8
2,642,592	+	ndk	b2518	0.32	-1.2
2,642,684	-				-1.0
2,642,740	-	ndk	b2518	0.66	-2.3
2,642,740	-	ndk	b2518	0.66	-3.1
2,642,809	+	ndk	b2518	0.82	-1.6
2,642,937	+				+0.4
2,643,036	-				-0.1
2,643,081	+				+0.1
2,643,131	-				-0.1
2,643,171	-				-0.0
2,643,235	-				+0.1
2,643,235	-				-0.3
2,643,567	+	pbpC	b2519	0.23	-0.8
2,643,567	+	pbpC	b2519	0.23	+0.1
2,643,567	+	pbpC	b2519	0.23	-0.6
2,643,575	-	pbpC	b2519	0.23	+0.1
2,643,627	+	pbpC	b2519	0.26	-1.0
2,643,645	-	pbpC	b2519	0.26	+0.2
2,643,690	+	pbpC	b2519	0.28	-0.5