Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uraA and upp are separated by 85 nucleotides upp and purM are separated by 324 nucleotides
b2497: uraA - uracil transporter (NCBI), at 2,616,893 to 2,618,182
uraA
b2498: upp - uracil phosphoribosyltransferase (VIMSS), at 2,618,268 to 2,618,894
upp
b2499: purM - phosphoribosylaminoimidazole synthetase (NCBI), at 2,619,219 to 2,620,256
purM
Position (kb)
2618
2619 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2617.336 kb on - strand, within uraA at 2617.346 kb on - strand, within uraA at 2617.391 kb on + strand, within uraA at 2617.399 kb on - strand, within uraA at 2617.399 kb on - strand, within uraA at 2617.404 kb on - strand, within uraA at 2617.436 kb on - strand, within uraA at 2617.492 kb on - strand, within uraA at 2617.572 kb on + strand, within uraA at 2617.675 kb on - strand, within uraA at 2617.675 kb on - strand, within uraA at 2617.690 kb on + strand, within uraA at 2617.695 kb on + strand, within uraA at 2617.907 kb on + strand, within uraA at 2617.926 kb on - strand, within uraA at 2618.007 kb on + strand, within uraA at 2618.029 kb on - strand, within uraA at 2618.031 kb on - strand, within uraA at 2618.155 kb on + strand at 2618.159 kb on - strand at 2618.164 kb on + strand at 2618.167 kb on - strand at 2618.184 kb on + strand at 2618.196 kb on + strand at 2618.209 kb on - strand at 2618.356 kb on + strand, within upp at 2618.361 kb on - strand, within upp at 2618.361 kb on - strand, within upp at 2618.361 kb on - strand, within upp at 2618.364 kb on - strand, within upp at 2618.402 kb on + strand, within upp at 2618.561 kb on + strand, within upp at 2618.648 kb on + strand, within upp at 2618.648 kb on + strand, within upp at 2618.729 kb on - strand, within upp at 2618.770 kb on + strand, within upp at 2618.778 kb on - strand, within upp at 2618.846 kb on + strand at 2618.889 kb on - strand at 2619.127 kb on + strand at 2619.127 kb on + strand at 2619.262 kb on + strand at 2619.284 kb on + strand at 2619.362 kb on + strand, within purM at 2619.501 kb on - strand, within purM at 2619.662 kb on + strand, within purM at 2619.662 kb on + strand, within purM at 2619.832 kb on + strand, within purM at 2619.832 kb on + strand, within purM at 2619.835 kb on - strand, within purM
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 2,617,336 - uraA b2497 0.34 -0.1 2,617,346 - uraA b2497 0.35 +0.2 2,617,391 + uraA b2497 0.39 +0.7 2,617,399 - uraA b2497 0.39 +0.2 2,617,399 - uraA b2497 0.39 +0.7 2,617,404 - uraA b2497 0.40 -0.5 2,617,436 - uraA b2497 0.42 +0.5 2,617,492 - uraA b2497 0.46 +1.1 2,617,572 + uraA b2497 0.53 -0.2 2,617,675 - uraA b2497 0.61 -0.2 2,617,675 - uraA b2497 0.61 -0.1 2,617,690 + uraA b2497 0.62 -0.1 2,617,695 + uraA b2497 0.62 +1.1 2,617,907 + uraA b2497 0.79 -0.6 2,617,926 - uraA b2497 0.80 -0.2 2,618,007 + uraA b2497 0.86 +0.1 2,618,029 - uraA b2497 0.88 +0.3 2,618,031 - uraA b2497 0.88 -0.2 2,618,155 + -0.1 2,618,159 - +0.8 2,618,164 + -0.3 2,618,167 - -0.7 2,618,184 + +0.2 2,618,196 + -0.5 2,618,209 - -0.0 2,618,356 + upp b2498 0.14 -0.3 2,618,361 - upp b2498 0.15 +1.0 2,618,361 - upp b2498 0.15 +0.2 2,618,361 - upp b2498 0.15 -0.1 2,618,364 - upp b2498 0.15 +0.4 2,618,402 + upp b2498 0.21 -1.0 2,618,561 + upp b2498 0.47 +0.1 2,618,648 + upp b2498 0.61 -1.0 2,618,648 + upp b2498 0.61 +0.2 2,618,729 - upp b2498 0.74 -0.1 2,618,770 + upp b2498 0.80 +2.4 2,618,778 - upp b2498 0.81 -0.0 2,618,846 + -0.3 2,618,889 - -0.7 2,619,127 + -0.9 2,619,127 + -0.2 2,619,262 + -5.4 2,619,284 + -4.6 2,619,362 + purM b2499 0.14 -3.5 2,619,501 - purM b2499 0.27 -5.7 2,619,662 + purM b2499 0.43 -1.3 2,619,662 + purM b2499 0.43 -4.4 2,619,832 + purM b2499 0.59 -4.5 2,619,832 + purM b2499 0.59 -3.3 2,619,835 - purM b2499 0.59 -4.3
Or see this region's nucleotide sequence