Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisA and hisF overlap by 19 nucleotides hisF and hisI overlap by 7 nucleotides hisI and wzzB are separated by 95 nucleotides
b2024: hisA - N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS), at 2,093,149 to 2,093,886
hisA
b2025: hisF - imidazole glycerol phosphate synthase subunit HisF (NCBI), at 2,093,868 to 2,094,644
hisF
b2026: hisI - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (NCBI), at 2,094,638 to 2,095,249
hisI
b2027: wzzB - regulator of length of O-antigen component of lipopolysaccharide chains (VIMSS), at 2,095,345 to 2,096,325
wzzB
Position (kb)
2094
2095
2096 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2093.818 kb on - strand at 2093.818 kb on - strand at 2093.881 kb on + strand at 2093.918 kb on + strand at 2093.978 kb on + strand, within hisF at 2094.236 kb on + strand, within hisF at 2094.236 kb on + strand, within hisF at 2094.236 kb on + strand, within hisF at 2094.244 kb on - strand, within hisF at 2094.244 kb on - strand, within hisF at 2094.248 kb on - strand, within hisF at 2094.338 kb on - strand, within hisF at 2094.596 kb on + strand at 2094.596 kb on + strand at 2094.647 kb on + strand at 2094.712 kb on - strand, within hisI at 2094.712 kb on - strand, within hisI at 2094.716 kb on + strand, within hisI at 2094.869 kb on + strand, within hisI at 2094.907 kb on - strand, within hisI at 2094.907 kb on - strand, within hisI at 2095.032 kb on + strand, within hisI at 2095.033 kb on + strand, within hisI at 2095.033 kb on + strand, within hisI at 2095.124 kb on + strand, within hisI at 2095.167 kb on + strand, within hisI at 2095.202 kb on - strand at 2095.231 kb on + strand at 2095.386 kb on - strand at 2095.490 kb on + strand, within wzzB at 2095.494 kb on + strand, within wzzB at 2095.586 kb on - strand, within wzzB at 2095.586 kb on - strand, within wzzB at 2095.606 kb on - strand, within wzzB at 2095.719 kb on - strand, within wzzB at 2095.719 kb on - strand, within wzzB at 2095.729 kb on - strand, within wzzB at 2095.819 kb on + strand, within wzzB at 2095.819 kb on + strand, within wzzB at 2095.900 kb on + strand, within wzzB at 2095.900 kb on + strand, within wzzB at 2096.075 kb on + strand, within wzzB at 2096.075 kb on + strand, within wzzB at 2096.102 kb on + strand, within wzzB at 2096.163 kb on - strand, within wzzB
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 2,093,818 - -3.0 2,093,818 - -3.0 2,093,881 + -2.8 2,093,918 + -3.4 2,093,978 + hisF b2025 0.14 -2.0 2,094,236 + hisF b2025 0.47 -3.0 2,094,236 + hisF b2025 0.47 -3.0 2,094,236 + hisF b2025 0.47 -3.5 2,094,244 - hisF b2025 0.48 -0.7 2,094,244 - hisF b2025 0.48 -4.4 2,094,248 - hisF b2025 0.49 -2.3 2,094,338 - hisF b2025 0.60 -1.3 2,094,596 + -1.6 2,094,596 + -0.5 2,094,647 + -0.1 2,094,712 - hisI b2026 0.12 -2.7 2,094,712 - hisI b2026 0.12 -1.2 2,094,716 + hisI b2026 0.13 -3.6 2,094,869 + hisI b2026 0.38 -2.6 2,094,907 - hisI b2026 0.44 -2.8 2,094,907 - hisI b2026 0.44 -2.0 2,095,032 + hisI b2026 0.64 -3.0 2,095,033 + hisI b2026 0.65 -2.6 2,095,033 + hisI b2026 0.65 -3.1 2,095,124 + hisI b2026 0.79 -1.8 2,095,167 + hisI b2026 0.86 -2.3 2,095,202 - -2.9 2,095,231 + -0.4 2,095,386 - -0.0 2,095,490 + wzzB b2027 0.15 +1.0 2,095,494 + wzzB b2027 0.15 +0.7 2,095,586 - wzzB b2027 0.25 +0.8 2,095,586 - wzzB b2027 0.25 -0.3 2,095,606 - wzzB b2027 0.27 -0.2 2,095,719 - wzzB b2027 0.38 -0.8 2,095,719 - wzzB b2027 0.38 -0.1 2,095,729 - wzzB b2027 0.39 -0.4 2,095,819 + wzzB b2027 0.48 -0.2 2,095,819 + wzzB b2027 0.48 +0.0 2,095,900 + wzzB b2027 0.57 -1.0 2,095,900 + wzzB b2027 0.57 +0.3 2,096,075 + wzzB b2027 0.74 +0.1 2,096,075 + wzzB b2027 0.74 +0.0 2,096,102 + wzzB b2027 0.77 -0.2 2,096,163 - wzzB b2027 0.83 +1.0
Or see this region's nucleotide sequence