Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b1297 and ycjL are separated by 211 nucleotides ycjL and puuR are separated by 26 nucleotides puuR and puuC are separated by 274 nucleotides
b1297: b1297 - putative glutamine synthetase (EC 6.3.1.2) (VIMSS), at 1,357,514 to 1,358,932
b1297
b1298: ycjL - probable amidotransferase subunit (VIMSS), at 1,359,144 to 1,359,908
ycjL
b1299: puuR - DNA-binding transcriptional repressor (NCBI), at 1,359,935 to 1,360,492
puuR
b1300: puuC - gamma-Glu-gamma-aminobutyraldehyde dehydrogenase, NAD(P)H-dependent (NCBI), at 1,360,767 to 1,362,254
puuC
Position (kb)
1359
1360 Strain fitness (log2 ratio)
-1
0
1 at 1358.414 kb on + strand, within b1297 at 1358.462 kb on + strand, within b1297 at 1358.477 kb on - strand, within b1297 at 1358.559 kb on + strand, within b1297 at 1358.640 kb on - strand, within b1297 at 1358.640 kb on - strand, within b1297 at 1358.860 kb on + strand at 1358.868 kb on - strand at 1359.236 kb on + strand, within ycjL at 1359.236 kb on + strand, within ycjL at 1359.453 kb on + strand, within ycjL at 1359.594 kb on + strand, within ycjL at 1359.759 kb on - strand, within ycjL at 1360.081 kb on - strand, within puuR at 1360.084 kb on - strand, within puuR at 1360.317 kb on - strand, within puuR at 1360.332 kb on - strand, within puuR at 1360.334 kb on + strand, within puuR at 1360.413 kb on - strand, within puuR at 1360.478 kb on - strand at 1360.478 kb on - strand at 1360.521 kb on - strand at 1360.526 kb on - strand at 1360.526 kb on - strand at 1360.698 kb on - strand at 1360.698 kb on - strand at 1360.761 kb on - strand at 1360.903 kb on - strand at 1360.903 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 1,358,414 + b1297 b1297 0.63 -0.1 1,358,462 + b1297 b1297 0.67 +0.3 1,358,477 - b1297 b1297 0.68 +0.5 1,358,559 + b1297 b1297 0.74 -0.3 1,358,640 - b1297 b1297 0.79 -0.3 1,358,640 - b1297 b1297 0.79 -0.3 1,358,860 + +0.4 1,358,868 - -0.0 1,359,236 + ycjL b1298 0.12 -0.1 1,359,236 + ycjL b1298 0.12 -0.1 1,359,453 + ycjL b1298 0.40 -0.1 1,359,594 + ycjL b1298 0.59 +0.0 1,359,759 - ycjL b1298 0.80 +0.1 1,360,081 - puuR b1299 0.26 -0.6 1,360,084 - puuR b1299 0.27 +0.4 1,360,317 - puuR b1299 0.68 -0.1 1,360,332 - puuR b1299 0.71 +0.9 1,360,334 + puuR b1299 0.72 +0.0 1,360,413 - puuR b1299 0.86 +1.1 1,360,478 - -0.3 1,360,478 - +0.1 1,360,521 - +0.7 1,360,526 - -0.7 1,360,526 - +0.2 1,360,698 - -0.2 1,360,698 - -0.3 1,360,761 - +0.5 1,360,903 - +0.0 1,360,903 - -0.5
Or see this region's nucleotide sequence