Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpB and trpC are separated by 11 nucleotides trpC and trpD are separated by 3 nucleotides
b1261: trpB - tryptophan synthase subunit beta (NCBI), at 1,315,246 to 1,316,439
trpB
b1262: trpC - N-(5-phosphoribosyl)anthranilate isomerase and indole-3-glycerolphosphate synthetase (VIMSS), at 1,316,451 to 1,317,809
trpC
b1263: trpD - bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI), at 1,317,813 to 1,319,408
trpD
Position (kb)
1316
1317
1318 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1315.453 kb on + strand, within trpB at 1315.671 kb on + strand, within trpB at 1315.984 kb on - strand, within trpB at 1316.375 kb on - strand at 1316.375 kb on - strand at 1316.600 kb on + strand, within trpC at 1316.692 kb on + strand, within trpC at 1316.692 kb on + strand, within trpC at 1317.035 kb on - strand, within trpC at 1317.035 kb on - strand, within trpC at 1317.113 kb on - strand, within trpC at 1317.144 kb on - strand, within trpC at 1317.153 kb on + strand, within trpC at 1317.181 kb on + strand, within trpC at 1317.305 kb on - strand, within trpC at 1317.389 kb on + strand, within trpC at 1317.389 kb on + strand, within trpC at 1317.643 kb on + strand, within trpC at 1317.684 kb on - strand at 1317.684 kb on - strand at 1317.766 kb on - strand at 1318.316 kb on + strand, within trpD at 1318.316 kb on + strand, within trpD
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 1,315,453 + trpB b1261 0.17 -2.0 1,315,671 + trpB b1261 0.36 -4.0 1,315,984 - trpB b1261 0.62 -4.5 1,316,375 - -4.6 1,316,375 - -3.5 1,316,600 + trpC b1262 0.11 -4.3 1,316,692 + trpC b1262 0.18 -2.8 1,316,692 + trpC b1262 0.18 -3.2 1,317,035 - trpC b1262 0.43 +0.6 1,317,035 - trpC b1262 0.43 -0.3 1,317,113 - trpC b1262 0.49 -1.4 1,317,144 - trpC b1262 0.51 -0.4 1,317,153 + trpC b1262 0.52 -3.3 1,317,181 + trpC b1262 0.54 -4.1 1,317,305 - trpC b1262 0.63 +0.2 1,317,389 + trpC b1262 0.69 -2.9 1,317,389 + trpC b1262 0.69 -4.6 1,317,643 + trpC b1262 0.88 -3.8 1,317,684 - -0.4 1,317,684 - -1.4 1,317,766 - +0.1 1,318,316 + trpD b1263 0.32 -3.4 1,318,316 + trpD b1263 0.32 -1.7
Or see this region's nucleotide sequence