Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yciF and yciG are separated by 85 nucleotides yciG and trpA are separated by 380 nucleotides trpA and trpB overlap by 1 nucleotides
b1258: yciF - hypothetical protein (NCBI), at 1,313,294 to 1,313,794
yciF
b1259: yciG - orf, hypothetical protein (VIMSS), at 1,313,880 to 1,314,059
yciG
b1260: trpA - tryptophan synthase subunit alpha (NCBI), at 1,314,440 to 1,315,246
trpA
b1261: trpB - tryptophan synthase subunit beta (NCBI), at 1,315,246 to 1,316,439
trpB
Position (kb)
1314
1315
1316 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1313.479 kb on - strand, within yciF at 1313.499 kb on - strand, within yciF at 1313.642 kb on + strand, within yciF at 1313.939 kb on - strand, within yciG at 1313.996 kb on - strand, within yciG at 1314.336 kb on + strand at 1314.440 kb on - strand at 1314.440 kb on - strand at 1314.440 kb on - strand at 1314.666 kb on + strand, within trpA at 1314.931 kb on - strand, within trpA at 1315.091 kb on - strand, within trpA at 1315.100 kb on - strand, within trpA at 1315.174 kb on + strand at 1315.218 kb on - strand at 1315.218 kb on - strand at 1315.453 kb on + strand, within trpB at 1315.671 kb on + strand, within trpB at 1315.984 kb on - strand, within trpB
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 1,313,479 - yciF b1258 0.37 +0.1 1,313,499 - yciF b1258 0.41 +1.1 1,313,642 + yciF b1258 0.69 +1.1 1,313,939 - yciG b1259 0.33 -0.5 1,313,996 - yciG b1259 0.64 +2.1 1,314,336 + +0.6 1,314,440 - -0.8 1,314,440 - -0.4 1,314,440 - +0.3 1,314,666 + trpA b1260 0.28 -5.8 1,314,931 - trpA b1260 0.61 -3.3 1,315,091 - trpA b1260 0.81 -4.9 1,315,100 - trpA b1260 0.82 -4.7 1,315,174 + -4.9 1,315,218 - -3.3 1,315,218 - -4.3 1,315,453 + trpB b1261 0.17 -2.0 1,315,671 + trpB b1261 0.36 -4.0 1,315,984 - trpB b1261 0.62 -4.5
Or see this region's nucleotide sequence