Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt putA and putP are separated by 422 nucleotides putP and efeU are separated by 542 nucleotides
b1014: putA - fused DNA-binding transcriptional regulator/proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase (NCBI), at 1,074,143 to 1,078,105
putA
b1015: putP - proline:sodium symporter (NCBI), at 1,078,528 to 1,080,036
putP
b4490: efeU - no description, at 1,080,579 to 1,081,408
efeU
Position (kb)
1078
1079
1080
1081 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1077.664 kb on - strand, within putA at 1077.664 kb on - strand, within putA at 1077.670 kb on - strand, within putA at 1077.670 kb on - strand, within putA at 1077.772 kb on - strand at 1077.874 kb on - strand at 1077.874 kb on - strand at 1077.880 kb on - strand at 1077.889 kb on - strand at 1077.912 kb on - strand at 1077.938 kb on - strand at 1077.953 kb on + strand at 1078.020 kb on + strand at 1078.086 kb on - strand at 1078.086 kb on - strand at 1078.144 kb on - strand at 1078.154 kb on - strand at 1078.162 kb on - strand at 1078.206 kb on - strand at 1078.229 kb on - strand at 1078.254 kb on + strand at 1078.333 kb on + strand at 1078.333 kb on + strand at 1078.395 kb on - strand at 1078.401 kb on + strand at 1078.401 kb on + strand at 1078.421 kb on + strand at 1078.435 kb on + strand at 1078.443 kb on - strand at 1078.488 kb on + strand at 1078.531 kb on + strand at 1078.531 kb on + strand at 1078.544 kb on - strand at 1078.549 kb on - strand at 1078.582 kb on + strand at 1078.592 kb on + strand at 1078.620 kb on - strand at 1078.651 kb on - strand at 1078.651 kb on - strand at 1078.651 kb on - strand at 1078.679 kb on - strand, within putP at 1078.689 kb on + strand, within putP at 1078.712 kb on - strand, within putP at 1078.771 kb on - strand, within putP at 1078.788 kb on + strand, within putP at 1078.788 kb on + strand, within putP at 1078.845 kb on + strand, within putP at 1078.902 kb on + strand, within putP at 1078.902 kb on + strand, within putP at 1078.958 kb on + strand, within putP at 1079.051 kb on - strand, within putP at 1079.051 kb on - strand, within putP at 1079.087 kb on + strand, within putP at 1079.100 kb on - strand, within putP at 1079.140 kb on + strand, within putP at 1079.255 kb on + strand, within putP at 1079.324 kb on + strand, within putP at 1079.360 kb on - strand, within putP at 1079.361 kb on - strand, within putP at 1079.361 kb on - strand, within putP at 1079.365 kb on - strand, within putP at 1079.381 kb on - strand, within putP at 1079.381 kb on - strand, within putP at 1079.514 kb on - strand, within putP at 1079.650 kb on + strand, within putP at 1079.750 kb on - strand, within putP at 1079.750 kb on - strand, within putP at 1079.770 kb on + strand, within putP at 1079.770 kb on + strand, within putP at 1079.773 kb on - strand, within putP at 1079.783 kb on - strand, within putP at 1079.873 kb on + strand, within putP at 1079.881 kb on - strand, within putP at 1079.940 kb on + strand at 1079.940 kb on + strand at 1079.943 kb on + strand at 1079.943 kb on + strand at 1080.086 kb on + strand at 1080.086 kb on + strand at 1080.094 kb on - strand at 1080.094 kb on - strand at 1080.229 kb on - strand at 1080.595 kb on + strand at 1080.684 kb on - strand, within efeU at 1080.696 kb on - strand, within efeU at 1080.701 kb on - strand, within efeU at 1080.787 kb on + strand, within efeU at 1080.823 kb on - strand, within efeU at 1080.823 kb on - strand, within efeU at 1080.898 kb on - strand, within efeU at 1080.907 kb on + strand, within efeU at 1080.924 kb on + strand, within efeU at 1080.924 kb on + strand, within efeU at 1080.957 kb on + strand, within efeU at 1081.013 kb on + strand, within efeU
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 1,077,664 - putA b1014 0.89 +0.4 1,077,664 - putA b1014 0.89 -0.1 1,077,670 - putA b1014 0.89 +0.1 1,077,670 - putA b1014 0.89 -0.8 1,077,772 - +0.6 1,077,874 - -0.5 1,077,874 - +0.2 1,077,880 - +0.7 1,077,889 - +0.0 1,077,912 - +0.1 1,077,938 - +0.2 1,077,953 + -0.2 1,078,020 + +0.3 1,078,086 - +0.0 1,078,086 - -0.3 1,078,144 - -0.4 1,078,154 - -1.0 1,078,162 - -0.2 1,078,206 - -0.5 1,078,229 - +0.3 1,078,254 + +0.6 1,078,333 + +0.0 1,078,333 + -0.5 1,078,395 - +0.5 1,078,401 + +0.2 1,078,401 + -0.1 1,078,421 + +0.1 1,078,435 + +0.6 1,078,443 - -0.5 1,078,488 + -0.3 1,078,531 + -0.7 1,078,531 + +0.2 1,078,544 - +0.5 1,078,549 - -0.2 1,078,582 + -0.7 1,078,592 + -0.0 1,078,620 - -0.4 1,078,651 - +0.3 1,078,651 - -1.7 1,078,651 - +0.3 1,078,679 - putP b1015 0.10 +0.1 1,078,689 + putP b1015 0.11 +0.1 1,078,712 - putP b1015 0.12 +1.0 1,078,771 - putP b1015 0.16 +1.8 1,078,788 + putP b1015 0.17 -0.2 1,078,788 + putP b1015 0.17 -0.7 1,078,845 + putP b1015 0.21 +0.3 1,078,902 + putP b1015 0.25 +0.7 1,078,902 + putP b1015 0.25 +0.0 1,078,958 + putP b1015 0.28 +0.0 1,079,051 - putP b1015 0.35 -0.3 1,079,051 - putP b1015 0.35 -0.1 1,079,087 + putP b1015 0.37 +0.5 1,079,100 - putP b1015 0.38 -0.2 1,079,140 + putP b1015 0.41 -0.4 1,079,255 + putP b1015 0.48 -0.1 1,079,324 + putP b1015 0.53 +0.7 1,079,360 - putP b1015 0.55 -0.3 1,079,361 - putP b1015 0.55 -0.3 1,079,361 - putP b1015 0.55 -0.3 1,079,365 - putP b1015 0.55 -0.0 1,079,381 - putP b1015 0.57 -0.3 1,079,381 - putP b1015 0.57 +0.1 1,079,514 - putP b1015 0.65 +0.7 1,079,650 + putP b1015 0.74 -0.6 1,079,750 - putP b1015 0.81 -0.4 1,079,750 - putP b1015 0.81 -0.5 1,079,770 + putP b1015 0.82 +0.2 1,079,770 + putP b1015 0.82 +0.1 1,079,773 - putP b1015 0.83 +0.2 1,079,783 - putP b1015 0.83 +1.5 1,079,873 + putP b1015 0.89 -0.5 1,079,881 - putP b1015 0.90 +0.1 1,079,940 + -0.7 1,079,940 + -0.9 1,079,943 + -0.5 1,079,943 + -0.1 1,080,086 + +0.2 1,080,086 + -0.1 1,080,094 - -0.1 1,080,094 - -2.4 1,080,229 - -0.5 1,080,595 + +0.1 1,080,684 - efeU b4490 0.13 +0.1 1,080,696 - efeU b4490 0.14 -0.2 1,080,701 - efeU b4490 0.15 +0.6 1,080,787 + efeU b4490 0.25 +0.1 1,080,823 - efeU b4490 0.29 -0.5 1,080,823 - efeU b4490 0.29 +0.0 1,080,898 - efeU b4490 0.38 +0.1 1,080,907 + efeU b4490 0.40 -0.1 1,080,924 + efeU b4490 0.42 -1.0 1,080,924 + efeU b4490 0.42 -0.3 1,080,957 + efeU b4490 0.46 -0.2 1,081,013 + efeU b4490 0.52 +0.4
Or see this region's nucleotide sequence