Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuC and leuB are separated by 2 nucleotides leuB and leuA overlap by 1 nucleotides
b0072: leuC - isopropylmalate isomerase large subunit (NCBI), at 79,464 to 80,864
leuC
b0073: leuB - 3-isopropylmalate dehydrogenase (VIMSS), at 80,867 to 81,958
leuB
b0074: leuA - 2-isopropylmalate synthase (NCBI), at 81,958 to 83,529
leuA
Position (kb)
80
81
82 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 79.883 kb on + strand, within leuC at 79.888 kb on + strand, within leuC at 79.896 kb on - strand, within leuC at 79.931 kb on + strand, within leuC at 80.049 kb on - strand, within leuC at 80.049 kb on - strand, within leuC at 80.077 kb on + strand, within leuC at 80.077 kb on + strand, within leuC at 80.092 kb on - strand, within leuC at 80.120 kb on - strand, within leuC at 80.120 kb on - strand, within leuC at 80.141 kb on + strand, within leuC at 80.141 kb on + strand, within leuC at 80.165 kb on + strand, within leuC at 80.210 kb on + strand, within leuC at 80.210 kb on + strand, within leuC at 80.218 kb on - strand, within leuC at 80.218 kb on - strand, within leuC at 80.236 kb on - strand, within leuC at 80.349 kb on + strand, within leuC at 80.387 kb on + strand at 80.387 kb on + strand, within leuC at 80.414 kb on - strand, within leuC at 80.438 kb on - strand, within leuC at 80.671 kb on - strand, within leuC at 80.671 kb on - strand, within leuC at 80.671 kb on - strand, within leuC at 80.671 kb on - strand, within leuC at 80.714 kb on - strand, within leuC at 80.714 kb on - strand, within leuC at 80.785 kb on + strand at 80.785 kb on + strand at 80.893 kb on - strand at 80.893 kb on - strand at 80.918 kb on + strand at 80.918 kb on + strand at 80.929 kb on - strand at 80.965 kb on + strand at 80.973 kb on - strand at 80.986 kb on - strand, within leuB at 81.032 kb on - strand, within leuB at 81.037 kb on + strand, within leuB at 81.114 kb on + strand, within leuB at 81.114 kb on + strand, within leuB at 81.122 kb on - strand, within leuB at 81.191 kb on - strand, within leuB at 81.191 kb on - strand, within leuB at 81.224 kb on + strand, within leuB at 81.284 kb on + strand, within leuB at 81.284 kb on + strand, within leuB at 81.287 kb on + strand, within leuB at 81.290 kb on - strand, within leuB at 81.292 kb on - strand, within leuB at 81.292 kb on - strand, within leuB at 81.314 kb on - strand, within leuB at 81.404 kb on + strand, within leuB at 81.409 kb on - strand, within leuB at 81.457 kb on + strand, within leuB at 81.480 kb on + strand, within leuB at 81.554 kb on - strand, within leuB at 81.580 kb on + strand, within leuB at 81.607 kb on - strand, within leuB at 81.692 kb on + strand, within leuB at 81.692 kb on + strand, within leuB at 81.910 kb on + strand at 81.910 kb on + strand at 81.922 kb on - strand at 82.051 kb on + strand at 82.135 kb on + strand, within leuA at 82.243 kb on + strand, within leuA at 82.243 kb on + strand, within leuA at 82.246 kb on - strand, within leuA at 82.425 kb on + strand, within leuA at 82.462 kb on - strand, within leuA at 82.462 kb on - strand, within leuA at 82.502 kb on - strand, within leuA at 82.506 kb on - strand, within leuA at 82.624 kb on + strand, within leuA at 82.632 kb on - strand, within leuA at 82.635 kb on + strand, within leuA at 82.637 kb on + strand, within leuA at 82.638 kb on - strand, within leuA at 82.638 kb on - strand, within leuA at 82.647 kb on + strand, within leuA at 82.671 kb on - strand, within leuA at 82.671 kb on - strand, within leuA at 82.671 kb on - strand, within leuA at 82.713 kb on + strand, within leuA at 82.860 kb on + strand, within leuA at 82.860 kb on + strand, within leuA at 82.870 kb on + strand, within leuA at 82.878 kb on - strand, within leuA
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 79,883 + leuC b0072 0.30 -2.1 79,888 + leuC b0072 0.30 -2.4 79,896 - leuC b0072 0.31 -2.1 79,931 + leuC b0072 0.33 -0.3 80,049 - leuC b0072 0.42 -1.7 80,049 - leuC b0072 0.42 -1.8 80,077 + leuC b0072 0.44 -2.2 80,077 + leuC b0072 0.44 -2.5 80,092 - leuC b0072 0.45 -4.4 80,120 - leuC b0072 0.47 -2.4 80,120 - leuC b0072 0.47 -2.8 80,141 + leuC b0072 0.48 -2.0 80,141 + leuC b0072 0.48 -2.5 80,165 + leuC b0072 0.50 -2.7 80,210 + leuC b0072 0.53 -2.0 80,210 + leuC b0072 0.53 -3.4 80,218 - leuC b0072 0.54 -1.9 80,218 - leuC b0072 0.54 -1.5 80,236 - leuC b0072 0.55 -2.2 80,349 + leuC b0072 0.63 -2.2 80,387 + -1.8 80,387 + leuC b0072 0.66 -2.6 80,414 - leuC b0072 0.68 -2.6 80,438 - leuC b0072 0.70 -1.9 80,671 - leuC b0072 0.86 -3.0 80,671 - leuC b0072 0.86 -1.6 80,671 - leuC b0072 0.86 -2.6 80,671 - leuC b0072 0.86 -2.0 80,714 - leuC b0072 0.89 -1.7 80,714 - leuC b0072 0.89 -2.8 80,785 + +1.0 80,785 + -1.5 80,893 - -1.9 80,893 - -2.5 80,918 + -3.2 80,918 + -2.7 80,929 - -1.6 80,965 + -1.5 80,973 - -2.4 80,986 - leuB b0073 0.11 -1.5 81,032 - leuB b0073 0.15 -2.1 81,037 + leuB b0073 0.16 -1.8 81,114 + leuB b0073 0.23 -3.4 81,114 + leuB b0073 0.23 -1.5 81,122 - leuB b0073 0.23 -1.7 81,191 - leuB b0073 0.30 -2.4 81,191 - leuB b0073 0.30 -1.4 81,224 + leuB b0073 0.33 -1.7 81,284 + leuB b0073 0.38 -1.4 81,284 + leuB b0073 0.38 -2.9 81,287 + leuB b0073 0.38 -2.6 81,290 - leuB b0073 0.39 -3.6 81,292 - leuB b0073 0.39 -2.0 81,292 - leuB b0073 0.39 -2.3 81,314 - leuB b0073 0.41 -2.9 81,404 + leuB b0073 0.49 -2.5 81,409 - leuB b0073 0.50 -1.7 81,457 + leuB b0073 0.54 -2.5 81,480 + leuB b0073 0.56 -2.1 81,554 - leuB b0073 0.63 -1.4 81,580 + leuB b0073 0.65 -2.0 81,607 - leuB b0073 0.68 -0.8 81,692 + leuB b0073 0.76 -3.7 81,692 + leuB b0073 0.76 -3.7 81,910 + -3.0 81,910 + -3.4 81,922 - -2.6 82,051 + -1.6 82,135 + leuA b0074 0.11 -1.1 82,243 + leuA b0074 0.18 +0.2 82,243 + leuA b0074 0.18 -2.4 82,246 - leuA b0074 0.18 -0.1 82,425 + leuA b0074 0.30 -3.3 82,462 - leuA b0074 0.32 -3.6 82,462 - leuA b0074 0.32 -0.4 82,502 - leuA b0074 0.35 -1.5 82,506 - leuA b0074 0.35 -1.7 82,624 + leuA b0074 0.42 -2.3 82,632 - leuA b0074 0.43 -3.4 82,635 + leuA b0074 0.43 -2.1 82,637 + leuA b0074 0.43 -2.5 82,638 - leuA b0074 0.43 -2.3 82,638 - leuA b0074 0.43 -2.3 82,647 + leuA b0074 0.44 -2.8 82,671 - leuA b0074 0.45 -2.2 82,671 - leuA b0074 0.45 -2.2 82,671 - leuA b0074 0.45 -2.9 82,713 + leuA b0074 0.48 -5.3 82,860 + leuA b0074 0.57 -0.7 82,860 + leuA b0074 0.57 -0.9 82,870 + leuA b0074 0.58 -2.5 82,878 - leuA b0074 0.59 -3.2
Or see this region's nucleotide sequence