Experiment: LB 0.5x with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yddE and narV are separated by 78 nucleotides narV and narW overlap by 4 nucleotides narW and narY overlap by 1 nucleotides
b1464: yddE - hypothetical protein (NCBI), at 1,532,989 to 1,533,882
yddE
b1465: narV - nitrate reductase 2 (NRZ), gamma subunit (NCBI), at 1,533,961 to 1,534,641
narV
b1466: narW - nitrate reductase 2 (NRZ), delta subunit (assembly subunit) (NCBI), at 1,534,638 to 1,535,333
narW
b1467: narY - nitrate reductase 2 (NRZ), beta subunit (NCBI), at 1,535,333 to 1,536,877
narY
Position (kb)
1534
1535
1536 Strain fitness (log2 ratio)
-1
0
1 at 1533.643 kb on - strand, within yddE at 1533.650 kb on + strand, within yddE at 1533.735 kb on + strand, within yddE at 1533.892 kb on + strand at 1533.947 kb on - strand at 1534.325 kb on - strand, within narV at 1534.336 kb on + strand, within narV at 1534.625 kb on + strand at 1534.659 kb on + strand at 1534.659 kb on + strand at 1534.776 kb on + strand, within narW at 1535.009 kb on + strand, within narW at 1535.035 kb on - strand, within narW at 1535.064 kb on - strand, within narW at 1535.075 kb on + strand, within narW at 1535.172 kb on - strand, within narW at 1535.310 kb on + strand at 1535.352 kb on - strand at 1535.352 kb on - strand at 1535.359 kb on + strand at 1535.563 kb on + strand, within narY at 1535.583 kb on - strand, within narY at 1535.843 kb on - strand, within narY at 1535.885 kb on + strand, within narY at 1536.005 kb on - strand, within narY at 1536.005 kb on - strand, within narY at 1536.015 kb on - strand, within narY at 1536.054 kb on + strand, within narY at 1536.316 kb on - strand, within narY at 1536.316 kb on - strand, within narY at 1536.323 kb on + strand, within narY
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 1,533,643 - yddE b1464 0.73 +0.2 1,533,650 + yddE b1464 0.74 -0.3 1,533,735 + yddE b1464 0.83 -0.1 1,533,892 + -0.0 1,533,947 - +0.6 1,534,325 - narV b1465 0.53 -0.1 1,534,336 + narV b1465 0.55 +0.7 1,534,625 + +0.2 1,534,659 + +0.5 1,534,659 + +1.0 1,534,776 + narW b1466 0.20 +0.4 1,535,009 + narW b1466 0.53 -0.6 1,535,035 - narW b1466 0.57 +0.1 1,535,064 - narW b1466 0.61 +0.3 1,535,075 + narW b1466 0.63 +0.3 1,535,172 - narW b1466 0.77 +0.5 1,535,310 + -0.2 1,535,352 - -0.2 1,535,352 - +0.4 1,535,359 + +0.5 1,535,563 + narY b1467 0.15 -0.1 1,535,583 - narY b1467 0.16 +0.0 1,535,843 - narY b1467 0.33 +0.2 1,535,885 + narY b1467 0.36 +0.2 1,536,005 - narY b1467 0.43 -0.2 1,536,005 - narY b1467 0.43 +0.2 1,536,015 - narY b1467 0.44 -0.8 1,536,054 + narY b1467 0.47 -0.1 1,536,316 - narY b1467 0.64 -0.2 1,536,316 - narY b1467 0.64 -0.8 1,536,323 + narY b1467 0.64 +0.3
Or see this region's nucleotide sequence