Experiment: LB 0.5x with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydiP and ydiQ are separated by 315 nucleotides ydiQ and ydiR are separated by 19 nucleotides
b1696: ydiP - predicted DNA-binding transcriptional regulator (NCBI), at 1,776,414 to 1,777,325
ydiP
b1697: ydiQ - hypothetical protein (NCBI), at 1,777,641 to 1,778,405
ydiQ
b1698: ydiR - predicted electron transfer flavoprotein, FAD-binding (NCBI), at 1,778,425 to 1,779,363
ydiR
Position (kb)
1777
1778
1779 Strain fitness (log2 ratio)
-1
0
1 at 1776.867 kb on - strand, within ydiP at 1777.038 kb on - strand, within ydiP at 1777.038 kb on - strand, within ydiP at 1777.038 kb on - strand, within ydiP at 1777.216 kb on + strand, within ydiP at 1777.304 kb on + strand at 1777.341 kb on - strand at 1777.347 kb on - strand at 1777.355 kb on + strand at 1777.413 kb on - strand at 1777.413 kb on - strand at 1777.449 kb on + strand at 1777.456 kb on + strand at 1777.548 kb on - strand at 1777.564 kb on + strand at 1777.618 kb on + strand at 1777.685 kb on - strand at 1777.690 kb on - strand at 1777.700 kb on + strand at 1777.704 kb on - strand at 1777.776 kb on - strand, within ydiQ at 1777.865 kb on - strand, within ydiQ at 1777.917 kb on - strand, within ydiQ at 1778.003 kb on + strand, within ydiQ at 1778.059 kb on + strand, within ydiQ at 1778.071 kb on + strand, within ydiQ at 1778.071 kb on + strand, within ydiQ at 1778.071 kb on + strand, within ydiQ at 1778.294 kb on + strand, within ydiQ at 1778.469 kb on + strand at 1778.740 kb on + strand, within ydiR at 1778.933 kb on - strand, within ydiR at 1779.145 kb on + strand, within ydiR at 1779.222 kb on - strand, within ydiR
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 1,776,867 - ydiP b1696 0.50 +0.0 1,777,038 - ydiP b1696 0.68 -0.8 1,777,038 - ydiP b1696 0.68 -1.6 1,777,038 - ydiP b1696 0.68 -0.0 1,777,216 + ydiP b1696 0.88 +1.0 1,777,304 + -1.3 1,777,341 - -0.3 1,777,347 - +0.0 1,777,355 + +0.0 1,777,413 - +0.4 1,777,413 - -0.6 1,777,449 + +1.0 1,777,456 + -0.0 1,777,548 - -0.1 1,777,564 + +1.6 1,777,618 + -0.6 1,777,685 - -0.3 1,777,690 - -0.7 1,777,700 + -0.3 1,777,704 - +0.1 1,777,776 - ydiQ b1697 0.18 +0.1 1,777,865 - ydiQ b1697 0.29 -0.1 1,777,917 - ydiQ b1697 0.36 -1.0 1,778,003 + ydiQ b1697 0.47 -0.8 1,778,059 + ydiQ b1697 0.55 +0.0 1,778,071 + ydiQ b1697 0.56 -1.7 1,778,071 + ydiQ b1697 0.56 -0.2 1,778,071 + ydiQ b1697 0.56 -1.5 1,778,294 + ydiQ b1697 0.85 -0.1 1,778,469 + +0.0 1,778,740 + ydiR b1698 0.34 +0.0 1,778,933 - ydiR b1698 0.54 -0.1 1,779,145 + ydiR b1698 0.77 -0.6 1,779,222 - ydiR b1698 0.85 +0.1
Or see this region's nucleotide sequence