Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mdoH and yceK are separated by 172 nucleotides yceK and msyB are separated by 0 nucleotides msyB and mdtG are separated by 82 nucleotides mdtG and lpxL are separated by 171 nucleotides
b1049: mdoH - glucosyltransferase MdoH (NCBI), at 1,110,086 to 1,112,629
mdoH
b1050: yceK - predicted lipoprotein (NCBI), at 1,112,802 to 1,113,029
yceK
b1051: msyB - acidic protein suppresses mutants lacking function of protein export (VIMSS), at 1,113,030 to 1,113,404
msyB
b1053: mdtG - predicted drug efflux system (NCBI), at 1,113,487 to 1,114,713
mdtG
b1054: lpxL - lipid A biosynthesis lauroyl acyltransferase (NCBI), at 1,114,885 to 1,115,805
lpxL
Position (kb)
1113
1114
1115 Strain fitness (log2 ratio)
-1
0
1
2 at 1112.561 kb on - strand at 1112.670 kb on - strand at 1112.866 kb on + strand, within yceK at 1113.040 kb on + strand at 1113.040 kb on + strand at 1113.311 kb on - strand, within msyB at 1113.311 kb on - strand, within msyB at 1113.450 kb on - strand at 1113.524 kb on - strand at 1113.533 kb on + strand at 1113.533 kb on + strand at 1113.665 kb on - strand at 1113.703 kb on - strand, within mdtG at 1113.816 kb on + strand, within mdtG at 1113.893 kb on - strand, within mdtG at 1113.915 kb on + strand, within mdtG at 1113.915 kb on + strand, within mdtG at 1114.083 kb on - strand, within mdtG at 1114.083 kb on - strand, within mdtG at 1114.104 kb on + strand, within mdtG at 1114.136 kb on + strand, within mdtG at 1114.136 kb on + strand, within mdtG at 1114.274 kb on + strand, within mdtG at 1114.411 kb on + strand, within mdtG at 1114.411 kb on + strand, within mdtG at 1114.437 kb on + strand, within mdtG at 1114.508 kb on - strand, within mdtG at 1114.600 kb on - strand at 1114.600 kb on - strand at 1114.703 kb on - strand at 1114.833 kb on + strand at 1114.846 kb on - strand at 1114.889 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 1,112,561 - +1.3 1,112,670 - -0.3 1,112,866 + yceK b1050 0.28 -0.9 1,113,040 + -0.7 1,113,040 + +0.7 1,113,311 - msyB b1051 0.75 +0.5 1,113,311 - msyB b1051 0.75 +0.7 1,113,450 - +0.3 1,113,524 - +0.6 1,113,533 + +1.5 1,113,533 + +0.7 1,113,665 - +1.1 1,113,703 - mdtG b1053 0.18 +0.6 1,113,816 + mdtG b1053 0.27 +1.7 1,113,893 - mdtG b1053 0.33 +0.9 1,113,915 + mdtG b1053 0.35 +1.2 1,113,915 + mdtG b1053 0.35 +1.2 1,114,083 - mdtG b1053 0.49 +0.8 1,114,083 - mdtG b1053 0.49 +1.3 1,114,104 + mdtG b1053 0.50 +1.0 1,114,136 + mdtG b1053 0.53 +1.5 1,114,136 + mdtG b1053 0.53 +0.7 1,114,274 + mdtG b1053 0.64 +0.6 1,114,411 + mdtG b1053 0.75 +1.6 1,114,411 + mdtG b1053 0.75 +1.3 1,114,437 + mdtG b1053 0.77 +0.8 1,114,508 - mdtG b1053 0.83 -0.2 1,114,600 - +0.8 1,114,600 - +0.9 1,114,703 - +1.0 1,114,833 + +2.5 1,114,846 - -0.9 1,114,889 - -0.6
Or see this region's nucleotide sequence