Strain Fitness in Escherichia coli BW25113 around b2498

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nturaA and upp are separated by 85 nucleotidesupp and purM are separated by 324 nucleotides b2497: uraA - uracil transporter (NCBI), at 2,616,893 to 2,618,182 uraA b2498: upp - uracil phosphoribosyltransferase (VIMSS), at 2,618,268 to 2,618,894 upp b2499: purM - phosphoribosylaminoimidazole synthetase (NCBI), at 2,619,219 to 2,620,256 purM Position (kb) 2618 2619Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 2617.336 kb on - strand, within uraAat 2617.346 kb on - strand, within uraAat 2617.391 kb on + strand, within uraAat 2617.399 kb on - strand, within uraAat 2617.399 kb on - strand, within uraAat 2617.404 kb on - strand, within uraAat 2617.436 kb on - strand, within uraAat 2617.492 kb on - strand, within uraAat 2617.572 kb on + strand, within uraAat 2617.675 kb on - strand, within uraAat 2617.675 kb on - strand, within uraAat 2617.690 kb on + strand, within uraAat 2617.695 kb on + strand, within uraAat 2617.907 kb on + strand, within uraAat 2617.926 kb on - strand, within uraAat 2618.007 kb on + strand, within uraAat 2618.029 kb on - strand, within uraAat 2618.031 kb on - strand, within uraAat 2618.155 kb on + strandat 2618.159 kb on - strandat 2618.164 kb on + strandat 2618.167 kb on - strandat 2618.184 kb on + strandat 2618.196 kb on + strandat 2618.209 kb on - strandat 2618.356 kb on + strand, within uppat 2618.361 kb on - strand, within uppat 2618.361 kb on - strand, within uppat 2618.361 kb on - strand, within uppat 2618.364 kb on - strand, within uppat 2618.402 kb on + strand, within uppat 2618.561 kb on + strand, within uppat 2618.648 kb on + strand, within uppat 2618.648 kb on + strand, within uppat 2618.729 kb on - strand, within uppat 2618.770 kb on + strand, within uppat 2618.778 kb on - strand, within uppat 2618.846 kb on + strandat 2618.889 kb on - strandat 2619.127 kb on + strandat 2619.127 kb on + strandat 2619.262 kb on + strandat 2619.284 kb on + strandat 2619.362 kb on + strand, within purMat 2619.501 kb on - strand, within purMat 2619.662 kb on + strand, within purMat 2619.662 kb on + strand, within purMat 2619.832 kb on + strand, within purMat 2619.832 kb on + strand, within purMat 2619.835 kb on - strand, within purM

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x
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2,617,336 - uraA b2497 0.34 -0.1
2,617,346 - uraA b2497 0.35 -0.0
2,617,391 + uraA b2497 0.39 +0.6
2,617,399 - uraA b2497 0.39 +0.7
2,617,399 - uraA b2497 0.39 +0.1
2,617,404 - uraA b2497 0.40 -0.6
2,617,436 - uraA b2497 0.42 +0.3
2,617,492 - uraA b2497 0.46 +0.4
2,617,572 + uraA b2497 0.53 +0.2
2,617,675 - uraA b2497 0.61 -0.0
2,617,675 - uraA b2497 0.61 +0.1
2,617,690 + uraA b2497 0.62 -0.6
2,617,695 + uraA b2497 0.62 +0.5
2,617,907 + uraA b2497 0.79 -0.4
2,617,926 - uraA b2497 0.80 +0.0
2,618,007 + uraA b2497 0.86 +0.0
2,618,029 - uraA b2497 0.88 -0.4
2,618,031 - uraA b2497 0.88 +0.0
2,618,155 + -0.3
2,618,159 - -0.4
2,618,164 + -0.3
2,618,167 - +0.2
2,618,184 + -0.0
2,618,196 + -0.3
2,618,209 - +0.3
2,618,356 + upp b2498 0.14 +1.1
2,618,361 - upp b2498 0.15 -0.1
2,618,361 - upp b2498 0.15 +1.8
2,618,361 - upp b2498 0.15 -0.4
2,618,364 - upp b2498 0.15 +0.3
2,618,402 + upp b2498 0.21 -0.1
2,618,561 + upp b2498 0.47 +0.1
2,618,648 + upp b2498 0.61 -2.1
2,618,648 + upp b2498 0.61 -0.9
2,618,729 - upp b2498 0.74 -1.9
2,618,770 + upp b2498 0.80 +0.3
2,618,778 - upp b2498 0.81 -1.3
2,618,846 + +0.2
2,618,889 - -2.4
2,619,127 + +0.5
2,619,127 + -0.2
2,619,262 + -5.5
2,619,284 + -3.7
2,619,362 + purM b2499 0.14 -5.1
2,619,501 - purM b2499 0.27 -4.2
2,619,662 + purM b2499 0.43 -1.4
2,619,662 + purM b2499 0.43 -4.5
2,619,832 + purM b2499 0.59 -4.6
2,619,832 + purM b2499 0.59 -3.4
2,619,835 - purM b2499 0.59 -4.4

Or see this region's nucleotide sequence