Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydcZ and yncA overlap by 4 nucleotides yncA and yncB are separated by 180 nucleotides yncB and b1450 are separated by 197 nucleotides b1450 and yncD are separated by 35 nucleotides
b1447: ydcZ - predicted inner membrane protein (NCBI), at 1,515,906 to 1,516,355
ydcZ
b1448: yncA - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 1,516,352 to 1,516,870
yncA
b1449: yncB - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (RefSeq), at 1,517,051 to 1,518,088
yncB
b1450: b1450 - orf, hypothetical protein (VIMSS), at 1,518,286 to 1,518,951
b1450
b1451: yncD - predicted iron outer membrane transporter (NCBI), at 1,518,987 to 1,521,089
yncD
Position (kb)
1517
1518
1519 Strain fitness (log2 ratio)
-1
0
1 at 1516.613 kb on - strand, within yncA at 1516.789 kb on - strand, within yncA at 1516.831 kb on + strand at 1516.870 kb on + strand at 1516.878 kb on - strand at 1517.165 kb on + strand, within yncB at 1517.165 kb on + strand, within yncB at 1517.246 kb on - strand, within yncB at 1517.310 kb on + strand, within yncB at 1517.341 kb on + strand, within yncB at 1517.341 kb on + strand, within yncB at 1517.496 kb on - strand, within yncB at 1517.517 kb on - strand, within yncB at 1517.635 kb on - strand, within yncB at 1517.635 kb on - strand, within yncB at 1517.739 kb on - strand, within yncB at 1517.739 kb on - strand, within yncB at 1517.741 kb on - strand, within yncB at 1517.805 kb on + strand, within yncB at 1517.805 kb on + strand, within yncB at 1517.839 kb on - strand, within yncB at 1517.839 kb on - strand, within yncB at 1517.923 kb on + strand, within yncB at 1517.923 kb on + strand, within yncB at 1517.950 kb on - strand, within yncB at 1517.950 kb on - strand, within yncB at 1518.047 kb on - strand at 1518.065 kb on - strand at 1518.067 kb on - strand at 1518.147 kb on + strand at 1518.175 kb on - strand at 1518.175 kb on - strand at 1518.200 kb on + strand at 1518.225 kb on - strand at 1518.237 kb on + strand at 1518.254 kb on + strand at 1518.295 kb on - strand at 1518.330 kb on + strand at 1518.338 kb on - strand at 1518.349 kb on - strand at 1518.354 kb on + strand, within b1450 at 1518.423 kb on + strand, within b1450 at 1518.425 kb on + strand, within b1450 at 1518.425 kb on + strand, within b1450 at 1518.425 kb on + strand, within b1450 at 1518.425 kb on + strand, within b1450 at 1518.495 kb on + strand, within b1450 at 1518.498 kb on + strand, within b1450 at 1518.506 kb on + strand, within b1450 at 1518.746 kb on - strand, within b1450 at 1518.756 kb on + strand, within b1450 at 1518.756 kb on + strand, within b1450 at 1518.767 kb on + strand, within b1450 at 1518.775 kb on - strand, within b1450 at 1518.779 kb on + strand, within b1450 at 1518.844 kb on + strand, within b1450 at 1518.844 kb on + strand, within b1450 at 1518.871 kb on + strand, within b1450 at 1518.872 kb on + strand, within b1450 at 1518.954 kb on - strand at 1518.974 kb on + strand at 1519.053 kb on - strand at 1519.084 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x remove 1,516,613 - yncA b1448 0.50 +0.2 1,516,789 - yncA b1448 0.84 -0.3 1,516,831 + -0.1 1,516,870 + -0.3 1,516,878 - -0.5 1,517,165 + yncB b1449 0.11 -0.4 1,517,165 + yncB b1449 0.11 -0.0 1,517,246 - yncB b1449 0.19 -0.6 1,517,310 + yncB b1449 0.25 -0.0 1,517,341 + yncB b1449 0.28 -0.3 1,517,341 + yncB b1449 0.28 -0.8 1,517,496 - yncB b1449 0.43 -0.1 1,517,517 - yncB b1449 0.45 -1.5 1,517,635 - yncB b1449 0.56 -1.3 1,517,635 - yncB b1449 0.56 +0.4 1,517,739 - yncB b1449 0.66 -1.2 1,517,739 - yncB b1449 0.66 -0.1 1,517,741 - yncB b1449 0.66 +0.5 1,517,805 + yncB b1449 0.73 -0.2 1,517,805 + yncB b1449 0.73 -0.6 1,517,839 - yncB b1449 0.76 -0.4 1,517,839 - yncB b1449 0.76 +0.8 1,517,923 + yncB b1449 0.84 -0.7 1,517,923 + yncB b1449 0.84 -0.9 1,517,950 - yncB b1449 0.87 +0.1 1,517,950 - yncB b1449 0.87 +0.8 1,518,047 - +1.1 1,518,065 - +1.1 1,518,067 - +1.1 1,518,147 + +0.1 1,518,175 - -0.5 1,518,175 - -0.4 1,518,200 + +0.9 1,518,225 - +0.3 1,518,237 + -0.1 1,518,254 + +0.5 1,518,295 - -0.6 1,518,330 + +0.1 1,518,338 - -0.3 1,518,349 - +1.1 1,518,354 + b1450 b1450 0.10 +0.5 1,518,423 + b1450 b1450 0.21 -0.2 1,518,425 + b1450 b1450 0.21 -0.4 1,518,425 + b1450 b1450 0.21 -1.1 1,518,425 + b1450 b1450 0.21 +0.1 1,518,425 + b1450 b1450 0.21 -1.5 1,518,495 + b1450 b1450 0.31 +0.1 1,518,498 + b1450 b1450 0.32 +0.6 1,518,506 + b1450 b1450 0.33 +0.5 1,518,746 - b1450 b1450 0.69 +1.1 1,518,756 + b1450 b1450 0.71 +0.2 1,518,756 + b1450 b1450 0.71 +0.3 1,518,767 + b1450 b1450 0.72 +0.1 1,518,775 - b1450 b1450 0.73 +1.1 1,518,779 + b1450 b1450 0.74 -0.2 1,518,844 + b1450 b1450 0.84 -0.5 1,518,844 + b1450 b1450 0.84 -0.9 1,518,871 + b1450 b1450 0.88 -0.4 1,518,872 + b1450 b1450 0.88 +0.1 1,518,954 - +0.1 1,518,974 + -0.9 1,519,053 - +0.2 1,519,084 - -0.8
Or see this region's nucleotide sequence