Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x; and D-Glucose 99.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt astD and astA overlap by 4 nucleotides astA and astC overlap by 4 nucleotides
b1746: astD - succinylglutamic semialdehyde dehydrogenase (NCBI), at 1,826,280 to 1,827,758
astD
b1747: astA - arginine succinyltransferase (NCBI), at 1,827,755 to 1,828,789
astA
b1748: astC - succinylornithine transaminase, PLP-dependent (NCBI), at 1,828,786 to 1,830,006
astC
Position (kb)
1827
1828
1829 Strain fitness (log2 ratio)
-1
0
1 at 1826.786 kb on + strand, within astD at 1826.848 kb on - strand, within astD at 1826.848 kb on - strand, within astD at 1826.870 kb on - strand, within astD at 1826.949 kb on - strand, within astD at 1826.949 kb on - strand, within astD at 1827.115 kb on + strand, within astD at 1827.262 kb on - strand, within astD at 1827.262 kb on - strand, within astD at 1827.350 kb on - strand, within astD at 1827.814 kb on + strand at 1827.814 kb on + strand at 1827.870 kb on - strand, within astA at 1827.875 kb on + strand, within astA at 1828.010 kb on - strand, within astA at 1828.010 kb on - strand, within astA at 1828.024 kb on - strand, within astA at 1828.076 kb on - strand, within astA at 1828.076 kb on - strand, within astA at 1828.129 kb on - strand, within astA at 1828.236 kb on + strand, within astA at 1828.424 kb on - strand, within astA at 1828.629 kb on + strand, within astA at 1828.765 kb on + strand at 1829.079 kb on + strand, within astC at 1829.172 kb on + strand, within astC at 1829.172 kb on + strand, within astC at 1829.304 kb on - strand, within astC at 1829.304 kb on - strand, within astC at 1829.492 kb on - strand, within astC at 1829.492 kb on - strand, within astC
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x; and D-Glucose 99.9 mM remove 1,826,786 + astD b1746 0.34 +0.7 1,826,848 - astD b1746 0.38 +0.3 1,826,848 - astD b1746 0.38 -0.0 1,826,870 - astD b1746 0.40 +0.6 1,826,949 - astD b1746 0.45 -0.5 1,826,949 - astD b1746 0.45 -0.2 1,827,115 + astD b1746 0.56 +0.3 1,827,262 - astD b1746 0.66 +0.3 1,827,262 - astD b1746 0.66 -0.2 1,827,350 - astD b1746 0.72 -0.1 1,827,814 + +0.2 1,827,814 + +0.9 1,827,870 - astA b1747 0.11 -0.1 1,827,875 + astA b1747 0.12 +0.4 1,828,010 - astA b1747 0.25 +0.4 1,828,010 - astA b1747 0.25 +0.4 1,828,024 - astA b1747 0.26 -0.2 1,828,076 - astA b1747 0.31 -0.9 1,828,076 - astA b1747 0.31 +0.6 1,828,129 - astA b1747 0.36 -0.7 1,828,236 + astA b1747 0.46 -1.0 1,828,424 - astA b1747 0.65 +0.1 1,828,629 + astA b1747 0.84 +0.2 1,828,765 + -0.2 1,829,079 + astC b1748 0.24 -0.8 1,829,172 + astC b1748 0.32 -1.2 1,829,172 + astC b1748 0.32 +0.1 1,829,304 - astC b1748 0.42 +0.0 1,829,304 - astC b1748 0.42 -0.1 1,829,492 - astC b1748 0.58 +0.1 1,829,492 - astC b1748 0.58 +0.2
Or see this region's nucleotide sequence