Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x; and D-Glucose 99.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rstB and tus are separated by 75 nucleotides tus and fumC overlap by 4 nucleotides
b1609: rstB - sensory histidine kinase in two-component regulatory system with RstA (NCBI), at 1,680,906 to 1,682,207
rstB
b1610: tus - DNA replication terminus site-binding protein (NCBI), at 1,682,283 to 1,683,212
tus
b1611: fumC - fumarate hydratase (NCBI), at 1,683,209 to 1,684,612
fumC
Position (kb)
1682
1683
1684 Strain fitness (log2 ratio)
-1
0
1
2 at 1681.555 kb on + strand, within rstB at 1681.555 kb on + strand, within rstB at 1681.779 kb on - strand, within rstB at 1681.779 kb on - strand, within rstB at 1681.827 kb on + strand, within rstB at 1682.011 kb on + strand, within rstB at 1682.068 kb on + strand, within rstB at 1682.068 kb on + strand, within rstB at 1682.090 kb on + strand at 1682.098 kb on - strand at 1682.100 kb on - strand at 1682.102 kb on + strand at 1682.102 kb on + strand at 1682.223 kb on + strand at 1682.223 kb on + strand at 1682.306 kb on - strand at 1682.451 kb on - strand, within tus at 1682.567 kb on - strand, within tus at 1682.620 kb on + strand, within tus at 1682.620 kb on + strand, within tus at 1682.820 kb on + strand, within tus at 1682.838 kb on + strand, within tus at 1682.878 kb on + strand, within tus at 1682.886 kb on - strand, within tus at 1682.941 kb on - strand, within tus at 1682.941 kb on - strand, within tus at 1683.046 kb on + strand, within tus at 1683.046 kb on + strand, within tus at 1683.207 kb on + strand at 1683.241 kb on + strand at 1683.295 kb on + strand at 1683.295 kb on + strand at 1683.439 kb on + strand, within fumC at 1683.595 kb on + strand, within fumC at 1683.612 kb on + strand, within fumC at 1683.612 kb on + strand, within fumC at 1683.665 kb on - strand, within fumC at 1683.665 kb on - strand, within fumC at 1683.709 kb on + strand, within fumC at 1683.709 kb on + strand, within fumC at 1683.859 kb on - strand, within fumC at 1683.859 kb on - strand, within fumC at 1683.981 kb on - strand, within fumC at 1684.016 kb on - strand, within fumC at 1684.016 kb on - strand, within fumC
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x; and D-Glucose 99.9 mM remove 1,681,555 + rstB b1609 0.50 +1.1 1,681,555 + rstB b1609 0.50 +0.6 1,681,779 - rstB b1609 0.67 +1.8 1,681,779 - rstB b1609 0.67 +1.7 1,681,827 + rstB b1609 0.71 +1.3 1,682,011 + rstB b1609 0.85 +0.3 1,682,068 + rstB b1609 0.89 +2.3 1,682,068 + rstB b1609 0.89 +0.6 1,682,090 + +1.3 1,682,098 - +0.4 1,682,100 - +0.3 1,682,102 + +0.8 1,682,102 + +0.8 1,682,223 + +1.2 1,682,223 + -0.6 1,682,306 - -0.2 1,682,451 - tus b1610 0.18 +0.7 1,682,567 - tus b1610 0.31 -0.8 1,682,620 + tus b1610 0.36 +0.7 1,682,620 + tus b1610 0.36 +0.1 1,682,820 + tus b1610 0.58 +0.5 1,682,838 + tus b1610 0.60 -0.0 1,682,878 + tus b1610 0.64 -0.9 1,682,886 - tus b1610 0.65 +0.6 1,682,941 - tus b1610 0.71 +0.0 1,682,941 - tus b1610 0.71 -0.6 1,683,046 + tus b1610 0.82 -0.4 1,683,046 + tus b1610 0.82 +0.4 1,683,207 + -1.3 1,683,241 + -0.2 1,683,295 + +0.0 1,683,295 + +0.3 1,683,439 + fumC b1611 0.16 -1.2 1,683,595 + fumC b1611 0.27 -0.5 1,683,612 + fumC b1611 0.29 -0.2 1,683,612 + fumC b1611 0.29 -0.2 1,683,665 - fumC b1611 0.32 -0.2 1,683,665 - fumC b1611 0.32 +0.2 1,683,709 + fumC b1611 0.36 -0.8 1,683,709 + fumC b1611 0.36 -0.6 1,683,859 - fumC b1611 0.46 +0.1 1,683,859 - fumC b1611 0.46 +0.8 1,683,981 - fumC b1611 0.55 +0.1 1,684,016 - fumC b1611 0.57 -0.1 1,684,016 - fumC b1611 0.57 +0.2
Or see this region's nucleotide sequence