Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yoeB and yefM overlap by 4 nucleotides yefM and hisL are separated by 282 nucleotides hisL and hisG are separated by 145 nucleotides hisG and hisD are separated by 5 nucleotides
b4539: yoeB - toxin of the YoeB-YefM toxin-antitoxin system (NCBI), at 2,087,235 to 2,087,489
yoeB
b2017: yefM - orf, hypothetical protein (VIMSS), at 2,087,486 to 2,087,737
yefM
b2018: hisL - his operon leader peptide (NCBI), at 2,088,020 to 2,088,070
hisL
b2019: hisG - ATP phosphoribosyltransferase (NCBI), at 2,088,216 to 2,089,115
hisG
b2020: hisD - histidinol dehydrogenase (NCBI), at 2,089,121 to 2,090,425
hisD
Position (kb)
2088
2089
2090 Strain fitness (log2 ratio)
-2
-1
0
1 at 2087.364 kb on - strand, within yoeB at 2087.506 kb on - strand at 2087.506 kb on - strand at 2087.544 kb on + strand, within yefM at 2087.642 kb on - strand, within yefM at 2087.867 kb on + strand at 2088.113 kb on - strand at 2088.203 kb on - strand at 2088.203 kb on - strand at 2088.316 kb on + strand, within hisG at 2088.484 kb on - strand, within hisG at 2088.602 kb on + strand, within hisG at 2088.610 kb on - strand, within hisG at 2088.610 kb on - strand, within hisG at 2088.657 kb on - strand, within hisG at 2088.681 kb on - strand, within hisG at 2088.859 kb on + strand, within hisG at 2088.900 kb on + strand, within hisG at 2088.900 kb on + strand, within hisG at 2089.029 kb on + strand at 2089.104 kb on + strand at 2089.133 kb on - strand at 2089.155 kb on - strand at 2089.187 kb on - strand at 2089.234 kb on - strand at 2089.234 kb on - strand at 2089.267 kb on + strand, within hisD at 2089.326 kb on + strand, within hisD at 2089.419 kb on - strand, within hisD at 2089.504 kb on + strand, within hisD at 2089.605 kb on - strand, within hisD at 2089.755 kb on - strand, within hisD at 2089.776 kb on + strand, within hisD at 2089.844 kb on + strand, within hisD at 2089.929 kb on - strand, within hisD at 2089.929 kb on - strand, within hisD at 2090.018 kb on - strand, within hisD at 2090.018 kb on - strand, within hisD at 2090.075 kb on - strand, within hisD
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM remove 2,087,364 - yoeB b4539 0.51 +0.1 2,087,506 - +0.8 2,087,506 - +1.6 2,087,544 + yefM b2017 0.23 +1.2 2,087,642 - yefM b2017 0.62 +1.6 2,087,867 + +0.4 2,088,113 - +0.3 2,088,203 - -0.3 2,088,203 - +0.6 2,088,316 + hisG b2019 0.11 -0.8 2,088,484 - hisG b2019 0.30 -0.2 2,088,602 + hisG b2019 0.43 -1.2 2,088,610 - hisG b2019 0.44 -0.6 2,088,610 - hisG b2019 0.44 -0.5 2,088,657 - hisG b2019 0.49 +0.2 2,088,681 - hisG b2019 0.52 +0.1 2,088,859 + hisG b2019 0.71 -0.2 2,088,900 + hisG b2019 0.76 -0.3 2,088,900 + hisG b2019 0.76 -0.4 2,089,029 + -0.5 2,089,104 + -0.3 2,089,133 - +0.0 2,089,155 - -0.3 2,089,187 - -0.4 2,089,234 - -0.8 2,089,234 - -0.2 2,089,267 + hisD b2020 0.11 -0.5 2,089,326 + hisD b2020 0.16 -1.1 2,089,419 - hisD b2020 0.23 -0.4 2,089,504 + hisD b2020 0.29 +0.2 2,089,605 - hisD b2020 0.37 +0.0 2,089,755 - hisD b2020 0.49 +0.1 2,089,776 + hisD b2020 0.50 -0.4 2,089,844 + hisD b2020 0.55 +0.4 2,089,929 - hisD b2020 0.62 -0.1 2,089,929 - hisD b2020 0.62 -0.4 2,090,018 - hisD b2020 0.69 +0.3 2,090,018 - hisD b2020 0.69 +0.3 2,090,075 - hisD b2020 0.73 -2.4
Or see this region's nucleotide sequence