Strain Fitness in Escherichia coli BW25113 around b3366

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
3,493,582	-	nirB	b3365	0.61	-0.4
3,493,591	+	nirB	b3365	0.61	-1.0
3,493,599	-	nirB	b3365	0.62	-0.3
3,493,623	+	nirB	b3365	0.62	-0.1
3,493,623	+	nirB	b3365	0.62	-0.9
3,493,623	+	nirB	b3365	0.62	-0.8
3,493,724	+	nirB	b3365	0.66	-1.8
3,493,779	+	nirB	b3365	0.69	+0.5
3,493,794	-	nirB	b3365	0.69	+0.5
3,493,805	-	nirB	b3365	0.70	+0.6
3,493,805	-	nirB	b3365	0.70	-1.4
3,493,811	+	nirB	b3365	0.70	+0.1
3,493,882	+	nirB	b3365	0.73	+0.2
3,493,882	+	nirB	b3365	0.73	-1.6
3,493,959	+	nirB	b3365	0.76	+0.4
3,493,989	-	nirB	b3365	0.77	+0.2
3,494,041	-	nirB	b3365	0.79	+0.6
3,494,107	-	nirB	b3365	0.82	+0.6
3,494,107	-	nirB	b3365	0.82	-0.2
3,494,247	+	nirB	b3365	0.87	+1.0
3,494,347	+				+0.1
3,494,395	+				+0.2
3,494,434	+				+0.1
3,494,434	+				+0.7
3,494,452	-				-0.1
3,494,459	+				+0.2
3,494,488	+				-0.9
3,494,499	-				+0.1
3,494,499	-				-0.9
3,494,507	-				-0.0
3,494,507	-				-0.4
3,494,507	-				+0.2
3,494,540	-				-1.3
3,494,548	+				+0.6
3,494,564	-				+0.1
3,494,577	-				+0.6
3,494,577	-				-0.9
3,494,646	-	nirD	b3366	0.22	-1.5
3,494,742	-	nirD	b3366	0.52	-0.4
3,494,760	+	nirD	b3366	0.57	-0.5
3,494,765	+	nirD	b3366	0.59	-1.0
3,494,765	+	nirD	b3366	0.59	-0.1
3,494,765	+	nirD	b3366	0.59	-0.6
3,494,779	-	nirD	b3366	0.63	-0.8
3,494,888	-				-0.2
3,494,888	-				+0.6
3,494,939	+				+0.2
3,494,939	+				-1.1
3,494,947	-				+0.3
3,494,947	-				-0.1
3,495,048	+				+0.2
3,495,056	-				-0.1
3,495,062	-				+0.5
3,495,071	+				-0.2
3,495,079	-				+0.4
3,495,113	+	nirC	b3367	0.11	+0.2
3,495,113	+	nirC	b3367	0.11	+1.3
3,495,123	+	nirC	b3367	0.12	-0.8
3,495,124	-	nirC	b3367	0.12	+0.0
3,495,124	-	nirC	b3367	0.12	+0.3
3,495,129	+	nirC	b3367	0.13	-0.9
3,495,129	+	nirC	b3367	0.13	-0.4
3,495,131	+	nirC	b3367	0.13	+0.4
3,495,148	-	nirC	b3367	0.15	+0.1
3,495,148	-	nirC	b3367	0.15	-3.7
3,495,148	-	nirC	b3367	0.15	-0.8
3,495,148	-	nirC	b3367	0.15	-0.4
3,495,182	+	nirC	b3367	0.19	-0.2
3,495,195	-	nirC	b3367	0.21	+0.1
3,495,197	+	nirC	b3367	0.21	+0.8
3,495,197	+	nirC	b3367	0.21	+0.1
3,495,251	+	nirC	b3367	0.28	+0.7
3,495,251	+	nirC	b3367	0.28	+0.2
3,495,251	+	nirC	b3367	0.28	+0.4
3,495,259	-	nirC	b3367	0.29	+0.1
3,495,271	-	nirC	b3367	0.30	+0.2
3,495,308	+	nirC	b3367	0.35	-0.2
3,495,308	+	nirC	b3367	0.35	+0.9
3,495,311	-	nirC	b3367	0.35	-0.3
3,495,311	-	nirC	b3367	0.35	+0.3
3,495,353	-	nirC	b3367	0.41	-0.1
3,495,358	+	nirC	b3367	0.41	+0.2
3,495,388	+	nirC	b3367	0.45	-0.0
3,495,388	+	nirC	b3367	0.45	-0.6
3,495,388	+	nirC	b3367	0.45	-0.3
3,495,388	+	nirC	b3367	0.45	+0.1
3,495,388	+	nirC	b3367	0.45	-1.5
3,495,396	-	nirC	b3367	0.46	-0.0
3,495,491	+	nirC	b3367	0.58	-0.7
3,495,535	+	nirC	b3367	0.63	+0.3
3,495,579	-	nirC	b3367	0.69	+0.2
3,495,609	+	nirC	b3367	0.72	+0.1
3,495,623	-	nirC	b3367	0.74	+1.1
3,495,623	-	nirC	b3367	0.74	+0.3
3,495,626	+	nirC	b3367	0.74	+0.4
3,495,626	+	nirC	b3367	0.74	+0.4
3,495,645	+	nirC	b3367	0.77	-0.6
3,495,645	+	nirC	b3367	0.77	+0.4
3,495,653	+	nirC	b3367	0.78	+0.0
3,495,654	-	nirC	b3367	0.78	+0.8
3,495,678	-	nirC	b3367	0.81	-0.6
3,495,678	-	nirC	b3367	0.81	-2.9
3,495,702	+	nirC	b3367	0.84	-0.5
3,495,705	-	nirC	b3367	0.84	-1.0
3,495,710	-	nirC	b3367	0.85	-0.1
3,495,715	-	nirC	b3367	0.86	+0.1
3,495,717	+	nirC	b3367	0.86	+1.2
3,495,744	-	nirC	b3367	0.89	-0.3
3,495,832	-				-1.0
3,495,893	+				+0.5