Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pykF and lpp are separated by 310 nucleotides lpp and ynhG are separated by 63 nucleotides
b1676: pykF - pyruvate kinase (NCBI), at 1,753,722 to 1,755,134
pykF
b1677: lpp - murein lipoprotein (NCBI), at 1,755,445 to 1,755,681
lpp
b1678: ynhG - hypothetical protein (NCBI), at 1,755,745 to 1,756,749
ynhG
Position (kb)
1755
1756 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1754.581 kb on + strand, within pykF at 1754.581 kb on + strand, within pykF at 1754.992 kb on + strand, within pykF at 1755.104 kb on + strand at 1755.375 kb on + strand at 1755.410 kb on + strand at 1755.417 kb on + strand at 1755.476 kb on - strand, within lpp at 1755.478 kb on + strand, within lpp at 1755.485 kb on + strand, within lpp at 1755.576 kb on + strand, within lpp at 1755.662 kb on + strand at 1755.782 kb on - strand at 1755.985 kb on - strand, within ynhG at 1755.985 kb on - strand, within ynhG at 1756.008 kb on + strand, within ynhG at 1756.008 kb on + strand, within ynhG at 1756.016 kb on + strand, within ynhG at 1756.016 kb on - strand, within ynhG at 1756.016 kb on - strand, within ynhG at 1756.080 kb on + strand, within ynhG at 1756.080 kb on + strand, within ynhG at 1756.151 kb on - strand, within ynhG at 1756.195 kb on - strand, within ynhG at 1756.211 kb on + strand, within ynhG at 1756.285 kb on - strand, within ynhG at 1756.285 kb on - strand, within ynhG at 1756.445 kb on - strand, within ynhG at 1756.470 kb on + strand, within ynhG at 1756.612 kb on + strand, within ynhG at 1756.620 kb on - strand, within ynhG at 1756.620 kb on - strand, within ynhG
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM remove 1,754,581 + pykF b1676 0.61 -0.3 1,754,581 + pykF b1676 0.61 -3.1 1,754,992 + pykF b1676 0.90 -0.2 1,755,104 + -0.3 1,755,375 + -2.0 1,755,410 + -0.8 1,755,417 + -1.4 1,755,476 - lpp b1677 0.13 -3.6 1,755,478 + lpp b1677 0.14 -1.6 1,755,485 + lpp b1677 0.17 -2.2 1,755,576 + lpp b1677 0.55 -0.0 1,755,662 + -1.6 1,755,782 - +0.3 1,755,985 - ynhG b1678 0.24 -1.7 1,755,985 - ynhG b1678 0.24 -1.3 1,756,008 + ynhG b1678 0.26 +0.7 1,756,008 + ynhG b1678 0.26 +0.5 1,756,016 + ynhG b1678 0.27 -0.3 1,756,016 - ynhG b1678 0.27 +0.2 1,756,016 - ynhG b1678 0.27 +0.8 1,756,080 + ynhG b1678 0.33 +0.1 1,756,080 + ynhG b1678 0.33 +0.7 1,756,151 - ynhG b1678 0.40 +0.1 1,756,195 - ynhG b1678 0.45 +0.7 1,756,211 + ynhG b1678 0.46 +1.2 1,756,285 - ynhG b1678 0.54 -0.0 1,756,285 - ynhG b1678 0.54 +1.0 1,756,445 - ynhG b1678 0.70 +0.9 1,756,470 + ynhG b1678 0.72 +0.6 1,756,612 + ynhG b1678 0.86 +0.1 1,756,620 - ynhG b1678 0.87 -0.1 1,756,620 - ynhG b1678 0.87 -0.2
Or see this region's nucleotide sequence