Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfcA and mepA overlap by 1 nucleotides mepA and aroC are separated by 3 nucleotides aroC and yfcB are separated by 34 nucleotides
b2327: yfcA - conserved inner membrane protein (NCBI), at 2,442,773 to 2,443,582
yfcA
b2328: mepA - penicillin-insensitive murein endopeptidase (NCBI), at 2,443,582 to 2,444,406
mepA
b2329: aroC - chorismate synthase (NCBI), at 2,444,410 to 2,445,495
aroC
b2330: yfcB - putative adenine-specific methylase (VIMSS), at 2,445,530 to 2,446,462
yfcB
Position (kb)
2444
2445
2446 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2443.700 kb on - strand, within mepA at 2443.700 kb on - strand, within mepA at 2443.705 kb on + strand, within mepA at 2443.770 kb on + strand, within mepA at 2443.778 kb on - strand, within mepA at 2443.778 kb on - strand, within mepA at 2443.782 kb on - strand, within mepA at 2443.797 kb on - strand, within mepA at 2443.797 kb on - strand, within mepA at 2443.817 kb on - strand, within mepA at 2443.817 kb on - strand, within mepA at 2443.897 kb on + strand, within mepA at 2444.082 kb on - strand, within mepA at 2444.245 kb on - strand, within mepA at 2444.245 kb on - strand, within mepA at 2444.256 kb on + strand, within mepA at 2444.552 kb on - strand, within aroC at 2444.844 kb on + strand, within aroC at 2444.866 kb on - strand, within aroC at 2444.923 kb on - strand, within aroC at 2444.997 kb on - strand, within aroC at 2444.997 kb on - strand, within aroC at 2445.075 kb on + strand, within aroC at 2445.075 kb on + strand, within aroC at 2445.180 kb on + strand, within aroC at 2445.213 kb on + strand, within aroC at 2445.213 kb on + strand, within aroC at 2445.219 kb on - strand, within aroC at 2445.219 kb on - strand, within aroC at 2445.244 kb on + strand, within aroC at 2445.326 kb on + strand, within aroC at 2445.326 kb on + strand, within aroC at 2445.337 kb on - strand, within aroC at 2445.337 kb on - strand, within aroC at 2445.487 kb on - strand at 2445.492 kb on - strand at 2445.514 kb on - strand at 2445.514 kb on - strand at 2445.577 kb on + strand at 2445.660 kb on - strand, within yfcB at 2445.661 kb on + strand, within yfcB at 2445.767 kb on - strand, within yfcB at 2446.057 kb on - strand, within yfcB at 2446.131 kb on + strand, within yfcB at 2446.369 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 2,443,700 - mepA b2328 0.14 +0.3 2,443,700 - mepA b2328 0.14 -0.1 2,443,705 + mepA b2328 0.15 -0.2 2,443,770 + mepA b2328 0.23 -0.2 2,443,778 - mepA b2328 0.24 -0.3 2,443,778 - mepA b2328 0.24 -0.1 2,443,782 - mepA b2328 0.24 -0.1 2,443,797 - mepA b2328 0.26 -0.4 2,443,797 - mepA b2328 0.26 +0.1 2,443,817 - mepA b2328 0.28 -0.5 2,443,817 - mepA b2328 0.28 +0.0 2,443,897 + mepA b2328 0.38 -1.2 2,444,082 - mepA b2328 0.61 +0.2 2,444,245 - mepA b2328 0.80 +0.3 2,444,245 - mepA b2328 0.80 +0.4 2,444,256 + mepA b2328 0.82 +0.0 2,444,552 - aroC b2329 0.13 -2.4 2,444,844 + aroC b2329 0.40 -4.1 2,444,866 - aroC b2329 0.42 -3.9 2,444,923 - aroC b2329 0.47 -3.2 2,444,997 - aroC b2329 0.54 -2.3 2,444,997 - aroC b2329 0.54 -3.8 2,445,075 + aroC b2329 0.61 -2.4 2,445,075 + aroC b2329 0.61 -0.7 2,445,180 + aroC b2329 0.71 -2.6 2,445,213 + aroC b2329 0.74 -3.4 2,445,213 + aroC b2329 0.74 -3.6 2,445,219 - aroC b2329 0.74 -4.0 2,445,219 - aroC b2329 0.74 -2.9 2,445,244 + aroC b2329 0.77 -3.2 2,445,326 + aroC b2329 0.84 -2.7 2,445,326 + aroC b2329 0.84 -4.6 2,445,337 - aroC b2329 0.85 -3.7 2,445,337 - aroC b2329 0.85 -2.7 2,445,487 - -3.3 2,445,492 - -3.9 2,445,514 - -0.5 2,445,514 - -0.6 2,445,577 + -0.4 2,445,660 - yfcB b2330 0.14 -0.5 2,445,661 + yfcB b2330 0.14 -1.1 2,445,767 - yfcB b2330 0.25 -1.4 2,446,057 - yfcB b2330 0.56 +0.9 2,446,131 + yfcB b2330 0.64 -0.7 2,446,369 - -0.4
Or see this region's nucleotide sequence