Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dadA and dadX are separated by 9 nucleotides dadX and b1191 are separated by 385 nucleotides
b1189: dadA - D-amino acid dehydrogenase (quinone) DadA (EC 1.4.5.1) (from data) , at 1,236,794 to 1,238,092
dadA
b1190: dadX - alanine racemase (NCBI), at 1,238,102 to 1,239,172
dadX
b1191: b1191 - orf, hypothetical protein (VIMSS), at 1,239,558 to 1,241,294
b1191
Position (kb)
1238
1239
1240 Strain fitness (log2 ratio)
-1
0
1
2 at 1237.146 kb on + strand, within dadA at 1237.180 kb on + strand, within dadA at 1237.213 kb on + strand, within dadA at 1237.213 kb on + strand, within dadA at 1237.218 kb on + strand, within dadA at 1237.507 kb on + strand, within dadA at 1237.545 kb on - strand, within dadA at 1237.545 kb on - strand, within dadA at 1237.803 kb on + strand, within dadA at 1237.803 kb on + strand, within dadA at 1238.119 kb on + strand at 1238.119 kb on + strand at 1238.234 kb on - strand, within dadX at 1238.234 kb on - strand, within dadX at 1238.275 kb on + strand, within dadX at 1238.275 kb on + strand, within dadX at 1238.292 kb on + strand, within dadX at 1238.803 kb on + strand, within dadX at 1238.803 kb on + strand, within dadX at 1238.855 kb on + strand, within dadX at 1238.874 kb on - strand, within dadX at 1238.920 kb on - strand, within dadX at 1238.920 kb on - strand, within dadX at 1239.281 kb on - strand at 1239.422 kb on - strand at 1239.639 kb on - strand at 1239.674 kb on + strand at 1239.674 kb on + strand at 1239.690 kb on - strand at 1239.769 kb on + strand, within b1191 at 1239.842 kb on + strand, within b1191 at 1239.886 kb on + strand, within b1191 at 1239.886 kb on + strand, within b1191 at 1239.977 kb on + strand, within b1191
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 1,237,146 + dadA b1189 0.27 +0.1 1,237,180 + dadA b1189 0.30 +0.0 1,237,213 + dadA b1189 0.32 -0.7 1,237,213 + dadA b1189 0.32 +0.8 1,237,218 + dadA b1189 0.33 +0.1 1,237,507 + dadA b1189 0.55 +0.6 1,237,545 - dadA b1189 0.58 +0.0 1,237,545 - dadA b1189 0.58 -0.3 1,237,803 + dadA b1189 0.78 +0.1 1,237,803 + dadA b1189 0.78 +0.3 1,238,119 + -0.0 1,238,119 + +0.5 1,238,234 - dadX b1190 0.12 +0.3 1,238,234 - dadX b1190 0.12 -0.1 1,238,275 + dadX b1190 0.16 -0.2 1,238,275 + dadX b1190 0.16 +0.3 1,238,292 + dadX b1190 0.18 +0.2 1,238,803 + dadX b1190 0.65 +0.7 1,238,803 + dadX b1190 0.65 +0.1 1,238,855 + dadX b1190 0.70 +0.4 1,238,874 - dadX b1190 0.72 +0.8 1,238,920 - dadX b1190 0.76 +2.1 1,238,920 - dadX b1190 0.76 +1.9 1,239,281 - -0.4 1,239,422 - -0.2 1,239,639 - -0.4 1,239,674 + -1.0 1,239,674 + -0.1 1,239,690 - +0.3 1,239,769 + b1191 b1191 0.12 +0.2 1,239,842 + b1191 b1191 0.16 +0.1 1,239,886 + b1191 b1191 0.19 -0.1 1,239,886 + b1191 b1191 0.19 -0.3 1,239,977 + b1191 b1191 0.24 -0.2
Or see this region's nucleotide sequence