Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfiO and yfiA are separated by 270 nucleotides yfiA and pheL are separated by 103 nucleotides pheL and pheA are separated by 98 nucleotides pheA and tyrA are separated by 42 nucleotides
b2595: yfiO - predicted lipoprotein (NCBI), at 2,734,168 to 2,734,905
yfiO
b2597: yfiA - cold shock protein associated with 30S ribosomal subunit (NCBI), at 2,735,176 to 2,735,517
yfiA
b2598: pheL - pheA gene leader peptide (NCBI), at 2,735,621 to 2,735,668
pheL
b2599: pheA - fused chorismate mutase P/prephenate dehydratase (NCBI), at 2,735,767 to 2,736,927
pheA
b2600: tyrA - fused chorismate mutase T/prephenate dehydrogenase (NCBI), at 2,736,970 to 2,738,091
tyrA
Position (kb)
2735
2736
2737 Strain fitness (log2 ratio)
-2
-1
0
1 at 2734.833 kb on + strand at 2734.848 kb on - strand at 2734.979 kb on + strand at 2734.996 kb on - strand at 2734.996 kb on - strand at 2735.339 kb on + strand, within yfiA at 2735.512 kb on + strand at 2735.520 kb on - strand at 2735.555 kb on - strand at 2735.555 kb on - strand at 2735.688 kb on + strand at 2735.719 kb on - strand at 2735.782 kb on + strand at 2735.782 kb on + strand at 2735.877 kb on + strand at 2735.877 kb on + strand at 2735.928 kb on - strand, within pheA at 2735.994 kb on + strand, within pheA at 2736.002 kb on - strand, within pheA at 2736.002 kb on - strand, within pheA at 2736.085 kb on + strand, within pheA at 2736.085 kb on + strand, within pheA at 2736.139 kb on - strand, within pheA at 2736.272 kb on + strand, within pheA at 2736.382 kb on + strand, within pheA at 2736.382 kb on + strand, within pheA at 2736.390 kb on - strand, within pheA at 2736.390 kb on - strand, within pheA at 2736.390 kb on - strand, within pheA at 2736.395 kb on - strand, within pheA at 2736.409 kb on - strand, within pheA at 2736.409 kb on - strand, within pheA at 2736.411 kb on - strand, within pheA at 2736.411 kb on - strand, within pheA at 2736.534 kb on - strand, within pheA at 2736.549 kb on - strand, within pheA at 2736.567 kb on - strand, within pheA at 2736.633 kb on + strand, within pheA at 2736.634 kb on - strand, within pheA at 2736.710 kb on + strand, within pheA at 2736.710 kb on + strand, within pheA at 2736.746 kb on + strand, within pheA at 2736.905 kb on - strand at 2736.942 kb on - strand at 2736.952 kb on - strand at 2737.058 kb on - strand at 2737.175 kb on - strand, within tyrA at 2737.294 kb on + strand, within tyrA at 2737.399 kb on - strand, within tyrA at 2737.589 kb on - strand, within tyrA at 2737.698 kb on - strand, within tyrA at 2737.698 kb on - strand, within tyrA at 2737.712 kb on - strand, within tyrA at 2737.753 kb on - strand, within tyrA at 2737.753 kb on - strand, within tyrA at 2737.877 kb on - strand, within tyrA at 2737.877 kb on - strand, within tyrA at 2737.890 kb on - strand, within tyrA
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x remove 2,734,833 + +0.0 2,734,848 - -1.3 2,734,979 + +0.3 2,734,996 - +0.0 2,734,996 - -0.3 2,735,339 + yfiA b2597 0.48 +0.1 2,735,512 + -0.4 2,735,520 - -0.6 2,735,555 - -1.9 2,735,555 - +0.1 2,735,688 + +0.5 2,735,719 - -0.8 2,735,782 + +0.2 2,735,782 + -0.2 2,735,877 + -0.2 2,735,877 + +0.2 2,735,928 - pheA b2599 0.14 -0.3 2,735,994 + pheA b2599 0.20 +0.0 2,736,002 - pheA b2599 0.20 +0.7 2,736,002 - pheA b2599 0.20 -0.3 2,736,085 + pheA b2599 0.27 +1.3 2,736,085 + pheA b2599 0.27 +0.5 2,736,139 - pheA b2599 0.32 +0.0 2,736,272 + pheA b2599 0.43 +0.3 2,736,382 + pheA b2599 0.53 +1.0 2,736,382 + pheA b2599 0.53 +0.7 2,736,390 - pheA b2599 0.54 +0.1 2,736,390 - pheA b2599 0.54 +0.4 2,736,390 - pheA b2599 0.54 +0.1 2,736,395 - pheA b2599 0.54 -0.8 2,736,409 - pheA b2599 0.55 -0.7 2,736,409 - pheA b2599 0.55 +0.4 2,736,411 - pheA b2599 0.55 +0.3 2,736,411 - pheA b2599 0.55 -1.9 2,736,534 - pheA b2599 0.66 -0.1 2,736,549 - pheA b2599 0.67 -0.9 2,736,567 - pheA b2599 0.69 -0.1 2,736,633 + pheA b2599 0.75 -0.2 2,736,634 - pheA b2599 0.75 +0.2 2,736,710 + pheA b2599 0.81 -0.2 2,736,710 + pheA b2599 0.81 -2.0 2,736,746 + pheA b2599 0.84 -0.5 2,736,905 - +0.3 2,736,942 - -0.1 2,736,952 - -1.2 2,737,058 - -0.4 2,737,175 - tyrA b2600 0.18 -0.7 2,737,294 + tyrA b2600 0.29 -1.2 2,737,399 - tyrA b2600 0.38 -0.6 2,737,589 - tyrA b2600 0.55 -0.8 2,737,698 - tyrA b2600 0.65 -1.5 2,737,698 - tyrA b2600 0.65 +0.5 2,737,712 - tyrA b2600 0.66 -0.8 2,737,753 - tyrA b2600 0.70 +0.1 2,737,753 - tyrA b2600 0.70 -0.7 2,737,877 - tyrA b2600 0.81 -0.4 2,737,877 - tyrA b2600 0.81 -0.3 2,737,890 - tyrA b2600 0.82 +0.5
Or see this region's nucleotide sequence