Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fhuC and fhuD overlap by 1 nucleotides fhuD and fhuB overlap by 4 nucleotides fhuB and hemL are separated by 157 nucleotides
b0151: fhuC - iron-hydroxamate transporter subunit (NCBI), at 169,778 to 170,575
fhuC
b0152: fhuD - iron-hydroxamate transporter subunit (NCBI), at 170,575 to 171,465
fhuD
b0153: fhuB - fused iron-hydroxamate transporter subunits of ABC superfamily: membrane components (NCBI), at 171,462 to 173,444
fhuB
b0154: hemL - glutamate-1-semialdehyde aminotransferase (NCBI), at 173,602 to 174,882
hemL
Position (kb)
171
172
173
174 Strain fitness (log2 ratio)
-2
-1
0
1 at 170.654 kb on - strand at 170.671 kb on + strand, within fhuD at 170.671 kb on + strand, within fhuD at 170.689 kb on - strand, within fhuD at 170.739 kb on + strand, within fhuD at 170.739 kb on + strand, within fhuD at 170.755 kb on - strand, within fhuD at 170.755 kb on - strand, within fhuD at 170.775 kb on + strand, within fhuD at 170.831 kb on + strand, within fhuD at 170.869 kb on + strand, within fhuD at 170.869 kb on + strand, within fhuD at 170.895 kb on - strand, within fhuD at 170.895 kb on - strand, within fhuD at 170.972 kb on - strand, within fhuD at 171.065 kb on + strand, within fhuD at 171.199 kb on + strand, within fhuD at 171.199 kb on + strand, within fhuD at 171.219 kb on + strand, within fhuD at 171.225 kb on + strand, within fhuD at 171.225 kb on + strand, within fhuD at 171.228 kb on + strand, within fhuD at 171.228 kb on + strand, within fhuD at 171.238 kb on - strand, within fhuD at 171.238 kb on - strand, within fhuD at 171.307 kb on - strand, within fhuD at 171.336 kb on + strand, within fhuD at 171.336 kb on + strand, within fhuD at 171.423 kb on + strand at 171.423 kb on - strand at 171.423 kb on - strand at 171.506 kb on + strand at 171.514 kb on - strand at 171.533 kb on - strand at 171.678 kb on + strand, within fhuB at 171.678 kb on + strand, within fhuB at 171.680 kb on + strand, within fhuB at 171.680 kb on + strand, within fhuB at 171.680 kb on - strand, within fhuB at 171.680 kb on - strand, within fhuB at 171.695 kb on + strand, within fhuB at 171.742 kb on + strand, within fhuB at 171.794 kb on + strand, within fhuB at 171.867 kb on + strand, within fhuB at 171.910 kb on + strand, within fhuB at 171.910 kb on + strand, within fhuB at 172.063 kb on + strand, within fhuB at 172.088 kb on - strand, within fhuB at 172.202 kb on + strand, within fhuB at 172.218 kb on - strand, within fhuB at 172.222 kb on - strand, within fhuB at 172.261 kb on + strand, within fhuB at 172.281 kb on + strand, within fhuB at 172.281 kb on + strand, within fhuB at 172.289 kb on + strand, within fhuB at 172.290 kb on - strand, within fhuB at 172.293 kb on + strand, within fhuB at 172.293 kb on + strand, within fhuB at 172.314 kb on + strand, within fhuB at 172.369 kb on - strand, within fhuB at 172.543 kb on - strand, within fhuB at 172.543 kb on - strand, within fhuB at 172.548 kb on + strand, within fhuB at 172.569 kb on - strand, within fhuB at 172.581 kb on + strand, within fhuB at 172.687 kb on + strand, within fhuB at 172.695 kb on - strand, within fhuB at 172.723 kb on + strand, within fhuB at 172.731 kb on - strand, within fhuB at 172.734 kb on + strand, within fhuB at 172.755 kb on + strand, within fhuB at 172.767 kb on + strand, within fhuB at 172.767 kb on + strand, within fhuB at 172.823 kb on - strand, within fhuB at 172.823 kb on - strand, within fhuB at 172.922 kb on - strand, within fhuB at 172.930 kb on + strand, within fhuB at 172.946 kb on - strand, within fhuB at 172.946 kb on - strand, within fhuB at 173.030 kb on + strand, within fhuB at 173.122 kb on - strand, within fhuB at 173.122 kb on - strand, within fhuB at 173.126 kb on - strand, within fhuB at 173.209 kb on + strand, within fhuB at 173.232 kb on - strand, within fhuB at 173.241 kb on + strand, within fhuB at 173.280 kb on + strand at 173.280 kb on + strand at 173.288 kb on - strand at 173.288 kb on - strand at 173.329 kb on + strand at 173.378 kb on - strand at 173.392 kb on + strand at 173.401 kb on - strand at 173.401 kb on - strand at 173.463 kb on + strand at 173.463 kb on - strand at 173.468 kb on + strand at 173.478 kb on + strand at 173.600 kb on + strand at 173.600 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 170,654 - -0.3 170,671 + fhuD b0152 0.11 -0.5 170,671 + fhuD b0152 0.11 -0.7 170,689 - fhuD b0152 0.13 -0.9 170,739 + fhuD b0152 0.18 -0.8 170,739 + fhuD b0152 0.18 -0.2 170,755 - fhuD b0152 0.20 -2.7 170,755 - fhuD b0152 0.20 +0.3 170,775 + fhuD b0152 0.22 -2.0 170,831 + fhuD b0152 0.29 -0.3 170,869 + fhuD b0152 0.33 -0.8 170,869 + fhuD b0152 0.33 +0.2 170,895 - fhuD b0152 0.36 +0.2 170,895 - fhuD b0152 0.36 -0.1 170,972 - fhuD b0152 0.45 -0.4 171,065 + fhuD b0152 0.55 +0.1 171,199 + fhuD b0152 0.70 +1.1 171,199 + fhuD b0152 0.70 -0.4 171,219 + fhuD b0152 0.72 -0.8 171,225 + fhuD b0152 0.73 -1.5 171,225 + fhuD b0152 0.73 -0.3 171,228 + fhuD b0152 0.73 -1.2 171,228 + fhuD b0152 0.73 -1.1 171,238 - fhuD b0152 0.74 -0.8 171,238 - fhuD b0152 0.74 -0.4 171,307 - fhuD b0152 0.82 -0.9 171,336 + fhuD b0152 0.85 -1.0 171,336 + fhuD b0152 0.85 -0.4 171,423 + -0.3 171,423 - -1.0 171,423 - -1.7 171,506 + -0.4 171,514 - -0.7 171,533 - -0.4 171,678 + fhuB b0153 0.11 -0.7 171,678 + fhuB b0153 0.11 -1.1 171,680 + fhuB b0153 0.11 -2.0 171,680 + fhuB b0153 0.11 -0.8 171,680 - fhuB b0153 0.11 -0.4 171,680 - fhuB b0153 0.11 -0.9 171,695 + fhuB b0153 0.12 -1.9 171,742 + fhuB b0153 0.14 -0.5 171,794 + fhuB b0153 0.17 -0.8 171,867 + fhuB b0153 0.20 -0.7 171,910 + fhuB b0153 0.23 -0.2 171,910 + fhuB b0153 0.23 -1.3 172,063 + fhuB b0153 0.30 -0.8 172,088 - fhuB b0153 0.32 -2.3 172,202 + fhuB b0153 0.37 -1.5 172,218 - fhuB b0153 0.38 -0.2 172,222 - fhuB b0153 0.38 -0.8 172,261 + fhuB b0153 0.40 -0.2 172,281 + fhuB b0153 0.41 -1.0 172,281 + fhuB b0153 0.41 -0.7 172,289 + fhuB b0153 0.42 -0.8 172,290 - fhuB b0153 0.42 -0.3 172,293 + fhuB b0153 0.42 -0.4 172,293 + fhuB b0153 0.42 -0.7 172,314 + fhuB b0153 0.43 -0.8 172,369 - fhuB b0153 0.46 -0.9 172,543 - fhuB b0153 0.55 -1.0 172,543 - fhuB b0153 0.55 -1.2 172,548 + fhuB b0153 0.55 -0.8 172,569 - fhuB b0153 0.56 -0.6 172,581 + fhuB b0153 0.56 -0.9 172,687 + fhuB b0153 0.62 -1.6 172,695 - fhuB b0153 0.62 -0.1 172,723 + fhuB b0153 0.64 -1.2 172,731 - fhuB b0153 0.64 -0.8 172,734 + fhuB b0153 0.64 -1.4 172,755 + fhuB b0153 0.65 -0.0 172,767 + fhuB b0153 0.66 -0.6 172,767 + fhuB b0153 0.66 -0.1 172,823 - fhuB b0153 0.69 -0.9 172,823 - fhuB b0153 0.69 -1.5 172,922 - fhuB b0153 0.74 -0.7 172,930 + fhuB b0153 0.74 -0.3 172,946 - fhuB b0153 0.75 +0.1 172,946 - fhuB b0153 0.75 -0.8 173,030 + fhuB b0153 0.79 -0.0 173,122 - fhuB b0153 0.84 -0.9 173,122 - fhuB b0153 0.84 -0.4 173,126 - fhuB b0153 0.84 -0.9 173,209 + fhuB b0153 0.88 -0.9 173,232 - fhuB b0153 0.89 -0.3 173,241 + fhuB b0153 0.90 -0.0 173,280 + -0.9 173,280 + -0.6 173,288 - -0.4 173,288 - -1.0 173,329 + -0.9 173,378 - -1.1 173,392 + +0.0 173,401 - -0.3 173,401 - -0.0 173,463 + +0.1 173,463 - -0.9 173,468 + -1.1 173,478 + -0.7 173,600 + -0.6 173,600 - -0.3
Or see this region's nucleotide sequence