Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygaH and mprA are separated by 90 nucleotides mprA and emrA are separated by 126 nucleotides emrA and emrB are separated by 16 nucleotides
b2683: ygaH - predicted inner membrane protein (NCBI), at 2,808,366 to 2,808,701
ygaH
b2684: mprA - DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI), at 2,808,792 to 2,809,322
mprA
b2685: emrA - multidrug efflux system (NCBI), at 2,809,449 to 2,810,621
emrA
b2686: emrB - multidrug efflux system protein (NCBI), at 2,810,638 to 2,812,176
emrB
Position (kb)
2809
2810
2811 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2808.515 kb on + strand, within ygaH at 2808.517 kb on - strand, within ygaH at 2808.533 kb on + strand, within ygaH at 2808.533 kb on + strand, within ygaH at 2808.534 kb on - strand, within ygaH at 2808.534 kb on - strand, within ygaH at 2808.560 kb on - strand, within ygaH at 2808.565 kb on - strand at 2808.645 kb on + strand, within ygaH at 2808.645 kb on + strand, within ygaH at 2808.645 kb on + strand, within ygaH at 2808.648 kb on - strand, within ygaH at 2808.655 kb on + strand, within ygaH at 2808.658 kb on - strand, within ygaH at 2808.660 kb on - strand, within ygaH at 2808.662 kb on + strand, within ygaH at 2808.662 kb on + strand, within ygaH at 2808.671 kb on - strand at 2808.876 kb on - strand, within mprA at 2809.100 kb on + strand, within mprA at 2809.100 kb on + strand, within mprA at 2809.107 kb on + strand, within mprA at 2809.200 kb on + strand, within mprA at 2809.287 kb on + strand at 2809.433 kb on - strand at 2809.497 kb on + strand at 2809.497 kb on + strand at 2809.546 kb on - strand at 2809.548 kb on - strand at 2809.748 kb on - strand, within emrA at 2809.748 kb on - strand, within emrA at 2809.806 kb on - strand, within emrA at 2809.931 kb on - strand, within emrA at 2810.041 kb on + strand, within emrA at 2810.097 kb on + strand, within emrA at 2810.097 kb on + strand, within emrA at 2810.097 kb on + strand, within emrA at 2810.100 kb on - strand, within emrA at 2810.100 kb on - strand, within emrA at 2810.103 kb on - strand, within emrA at 2810.127 kb on - strand, within emrA at 2810.147 kb on - strand, within emrA at 2810.204 kb on - strand, within emrA at 2810.320 kb on + strand, within emrA at 2810.339 kb on - strand, within emrA at 2810.417 kb on - strand, within emrA at 2810.443 kb on - strand, within emrA at 2810.480 kb on + strand, within emrA at 2810.482 kb on - strand, within emrA at 2810.482 kb on - strand, within emrA at 2810.503 kb on + strand, within emrA at 2810.533 kb on + strand at 2810.543 kb on + strand at 2810.543 kb on + strand at 2810.549 kb on - strand at 2810.621 kb on + strand at 2810.633 kb on + strand at 2810.716 kb on + strand at 2810.716 kb on + strand at 2810.724 kb on - strand at 2810.763 kb on - strand at 2810.764 kb on + strand at 2810.764 kb on + strand at 2810.921 kb on + strand, within emrB at 2811.019 kb on - strand, within emrB at 2811.019 kb on - strand, within emrB at 2811.034 kb on + strand, within emrB at 2811.034 kb on + strand, within emrB at 2811.151 kb on - strand, within emrB at 2811.196 kb on - strand, within emrB at 2811.196 kb on - strand, within emrB at 2811.310 kb on + strand, within emrB at 2811.431 kb on - strand, within emrB at 2811.436 kb on - strand, within emrB at 2811.505 kb on - strand, within emrB at 2811.507 kb on - strand, within emrB at 2811.507 kb on - strand, within emrB at 2811.528 kb on + strand at 2811.581 kb on - strand, within emrB
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x remove 2,808,515 + ygaH b2683 0.44 +0.3 2,808,517 - ygaH b2683 0.45 +0.4 2,808,533 + ygaH b2683 0.50 +0.1 2,808,533 + ygaH b2683 0.50 +0.3 2,808,534 - ygaH b2683 0.50 -0.5 2,808,534 - ygaH b2683 0.50 +0.1 2,808,560 - ygaH b2683 0.58 +0.5 2,808,565 - -1.0 2,808,645 + ygaH b2683 0.83 +0.3 2,808,645 + ygaH b2683 0.83 -0.0 2,808,645 + ygaH b2683 0.83 +0.3 2,808,648 - ygaH b2683 0.84 -0.2 2,808,655 + ygaH b2683 0.86 +0.2 2,808,658 - ygaH b2683 0.87 +0.5 2,808,660 - ygaH b2683 0.88 -0.6 2,808,662 + ygaH b2683 0.88 +1.0 2,808,662 + ygaH b2683 0.88 +0.1 2,808,671 - +0.1 2,808,876 - mprA b2684 0.16 +0.4 2,809,100 + mprA b2684 0.58 +3.4 2,809,100 + mprA b2684 0.58 +3.4 2,809,107 + mprA b2684 0.59 +3.0 2,809,200 + mprA b2684 0.77 +3.7 2,809,287 + +3.6 2,809,433 - +0.4 2,809,497 + -1.0 2,809,497 + +0.1 2,809,546 - -0.5 2,809,548 - -1.8 2,809,748 - emrA b2685 0.25 -1.8 2,809,748 - emrA b2685 0.25 -1.7 2,809,806 - emrA b2685 0.30 -1.3 2,809,931 - emrA b2685 0.41 -0.5 2,810,041 + emrA b2685 0.50 -2.6 2,810,097 + emrA b2685 0.55 -0.8 2,810,097 + emrA b2685 0.55 -0.5 2,810,097 + emrA b2685 0.55 -0.4 2,810,100 - emrA b2685 0.55 +0.2 2,810,100 - emrA b2685 0.55 -2.0 2,810,103 - emrA b2685 0.56 -0.2 2,810,127 - emrA b2685 0.58 -0.8 2,810,147 - emrA b2685 0.60 -1.0 2,810,204 - emrA b2685 0.64 -1.2 2,810,320 + emrA b2685 0.74 -0.4 2,810,339 - emrA b2685 0.76 +0.8 2,810,417 - emrA b2685 0.83 -1.0 2,810,443 - emrA b2685 0.85 -0.8 2,810,480 + emrA b2685 0.88 -0.7 2,810,482 - emrA b2685 0.88 -0.4 2,810,482 - emrA b2685 0.88 -1.6 2,810,503 + emrA b2685 0.90 -1.4 2,810,533 + -0.6 2,810,543 + -0.7 2,810,543 + -0.7 2,810,549 - -0.9 2,810,621 + -0.0 2,810,633 + -0.0 2,810,716 + -0.9 2,810,716 + -1.3 2,810,724 - -1.4 2,810,763 - -1.0 2,810,764 + -1.5 2,810,764 + -1.5 2,810,921 + emrB b2686 0.18 -1.4 2,811,019 - emrB b2686 0.25 -0.9 2,811,019 - emrB b2686 0.25 -2.1 2,811,034 + emrB b2686 0.26 -1.7 2,811,034 + emrB b2686 0.26 -1.0 2,811,151 - emrB b2686 0.33 -0.6 2,811,196 - emrB b2686 0.36 -0.4 2,811,196 - emrB b2686 0.36 -1.4 2,811,310 + emrB b2686 0.44 -1.8 2,811,431 - emrB b2686 0.52 -0.3 2,811,436 - emrB b2686 0.52 -0.3 2,811,505 - emrB b2686 0.56 -1.4 2,811,507 - emrB b2686 0.56 +0.2 2,811,507 - emrB b2686 0.56 -2.8 2,811,528 + +0.3 2,811,581 - emrB b2686 0.61 -0.1
Or see this region's nucleotide sequence