Strain Fitness in Escherichia coli BW25113 around b2213

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyojI and alkB are separated by 75 nucleotidesalkB and ada overlap by 1 nucleotidesada and yojL are separated by 73 nucleotides b2211: yojI - fused predicted multidrug transport subunits of ABC superfamily: membrane component/ATP-binding component (NCBI), at 2,304,994 to 2,306,637 yojI b2212: alkB - oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI), at 2,306,713 to 2,307,363 alkB b2213: ada - fused DNA-binding transcriptional dual regulator/O6-methylguanine-DNA methyltransferase (NCBI), at 2,307,363 to 2,308,427 ada b2214: yojL - predicted thiamine biosynthesis lipoprotein (NCBI), at 2,308,501 to 2,309,556 yojL Position (kb) 2307 2308 2309Strain fitness (log2 ratio) -2 -1 0 1 2at 2306.433 kb on - strand, within yojIat 2306.444 kb on - strand, within yojIat 2306.444 kb on - strand, within yojIat 2306.444 kb on - strand, within yojIat 2306.500 kb on + strandat 2306.572 kb on + strandat 2306.572 kb on + strandat 2306.783 kb on + strand, within alkBat 2306.801 kb on - strand, within alkBat 2306.942 kb on - strand, within alkBat 2306.942 kb on - strand, within alkBat 2307.059 kb on + strand, within alkBat 2307.168 kb on - strand, within alkBat 2307.181 kb on - strand, within alkBat 2307.336 kb on + strandat 2307.336 kb on + strandat 2307.336 kb on + strandat 2307.368 kb on - strandat 2307.393 kb on + strandat 2307.549 kb on - strand, within adaat 2307.562 kb on - strand, within adaat 2307.583 kb on + strand, within adaat 2307.593 kb on + strand, within adaat 2307.849 kb on - strand, within adaat 2308.153 kb on + strand, within adaat 2308.153 kb on + strand, within adaat 2308.162 kb on + strand, within adaat 2308.327 kb on + strandat 2308.337 kb on - strandat 2308.651 kb on + strand, within yojLat 2308.661 kb on - strandat 2308.661 kb on - strand, within yojLat 2308.745 kb on - strand, within yojLat 2308.784 kb on - strand, within yojLat 2308.796 kb on - strand, within yojLat 2308.916 kb on + strand, within yojLat 2308.916 kb on + strand, within yojLat 2308.964 kb on - strand, within yojLat 2308.964 kb on - strand, within yojLat 2309.070 kb on - strand, within yojLat 2309.100 kb on + strand, within yojLat 2309.123 kb on - strand, within yojLat 2309.286 kb on - strand, within yojLat 2309.286 kb on - strand, within yojL

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
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2,306,433 - yojI b2211 0.88 +0.5
2,306,444 - yojI b2211 0.88 +1.5
2,306,444 - yojI b2211 0.88 +0.7
2,306,444 - yojI b2211 0.88 +1.6
2,306,500 + +2.4
2,306,572 + -0.3
2,306,572 + +1.7
2,306,783 + alkB b2212 0.11 -0.7
2,306,801 - alkB b2212 0.14 +1.0
2,306,942 - alkB b2212 0.35 +0.5
2,306,942 - alkB b2212 0.35 +0.4
2,307,059 + alkB b2212 0.53 -0.4
2,307,168 - alkB b2212 0.70 +0.2
2,307,181 - alkB b2212 0.72 +0.6
2,307,336 + -0.3
2,307,336 + -0.5
2,307,336 + -0.8
2,307,368 - -0.9
2,307,393 + -1.0
2,307,549 - ada b2213 0.17 +0.3
2,307,562 - ada b2213 0.19 -0.9
2,307,583 + ada b2213 0.21 -1.3
2,307,593 + ada b2213 0.22 -0.9
2,307,849 - ada b2213 0.46 +0.3
2,308,153 + ada b2213 0.74 -0.2
2,308,153 + ada b2213 0.74 +0.1
2,308,162 + ada b2213 0.75 -0.1
2,308,327 + -0.0
2,308,337 - +0.5
2,308,651 + yojL b2214 0.14 +0.3
2,308,661 - -1.9
2,308,661 - yojL b2214 0.15 +0.8
2,308,745 - yojL b2214 0.23 +0.7
2,308,784 - yojL b2214 0.27 +0.7
2,308,796 - yojL b2214 0.28 +1.0
2,308,916 + yojL b2214 0.39 +0.4
2,308,916 + yojL b2214 0.39 +0.2
2,308,964 - yojL b2214 0.44 +0.9
2,308,964 - yojL b2214 0.44 +0.7
2,309,070 - yojL b2214 0.54 -0.1
2,309,100 + yojL b2214 0.57 -0.8
2,309,123 - yojL b2214 0.59 +0.3
2,309,286 - yojL b2214 0.74 +0.9
2,309,286 - yojL b2214 0.74 +0.6

Or see this region's nucleotide sequence