Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x; and D-Glucose 99.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt spy and astE are separated by 329 nucleotides astE and astB overlap by 8 nucleotides
b1743: spy - envelope stress induced periplasmic protein (NCBI), at 1,823,164 to 1,823,649
spy
b1744: astE - succinylglutamate desuccinylase (NCBI), at 1,823,979 to 1,824,947
astE
b1745: astB - succinylarginine dihydrolase (NCBI), at 1,824,940 to 1,826,283
astB
Position (kb)
1823
1824
1825 Strain fitness (log2 ratio)
-1
0
1 at 1823.108 kb on + strand at 1823.191 kb on - strand at 1823.293 kb on - strand, within spy at 1823.519 kb on + strand, within spy at 1823.549 kb on + strand, within spy at 1823.549 kb on + strand, within spy at 1823.559 kb on + strand, within spy at 1823.587 kb on + strand, within spy at 1823.595 kb on - strand, within spy at 1823.595 kb on - strand, within spy at 1823.613 kb on + strand at 1823.621 kb on - strand at 1823.629 kb on - strand at 1823.629 kb on - strand at 1823.683 kb on + strand at 1823.686 kb on + strand at 1823.703 kb on + strand at 1823.711 kb on - strand at 1823.729 kb on - strand at 1824.017 kb on - strand at 1824.017 kb on - strand at 1824.032 kb on + strand at 1824.047 kb on - strand at 1824.047 kb on - strand at 1824.052 kb on - strand at 1824.053 kb on - strand at 1824.059 kb on - strand at 1824.059 kb on - strand at 1824.087 kb on + strand, within astE at 1824.090 kb on - strand, within astE at 1824.090 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.330 kb on + strand, within astE at 1824.333 kb on - strand, within astE at 1824.380 kb on + strand, within astE at 1824.397 kb on + strand, within astE at 1824.512 kb on + strand, within astE at 1824.650 kb on - strand, within astE at 1824.857 kb on + strand at 1824.871 kb on - strand at 1825.089 kb on - strand, within astB at 1825.285 kb on + strand, within astB at 1825.285 kb on + strand, within astB at 1825.293 kb on - strand, within astB at 1825.321 kb on - strand, within astB at 1825.321 kb on - strand, within astB at 1825.378 kb on + strand, within astB at 1825.727 kb on - strand, within astB at 1825.767 kb on - strand, within astB
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x; and D-Glucose 99.9 mM remove 1,823,108 + -0.4 1,823,191 - +0.3 1,823,293 - spy b1743 0.27 +0.2 1,823,519 + spy b1743 0.73 -1.1 1,823,549 + spy b1743 0.79 -0.5 1,823,549 + spy b1743 0.79 -0.4 1,823,559 + spy b1743 0.81 +0.6 1,823,587 + spy b1743 0.87 +0.2 1,823,595 - spy b1743 0.89 +0.3 1,823,595 - spy b1743 0.89 -1.6 1,823,613 + +1.6 1,823,621 - -0.2 1,823,629 - +0.8 1,823,629 - +0.8 1,823,683 + -0.1 1,823,686 + -0.3 1,823,703 + +0.3 1,823,711 - +0.6 1,823,729 - +1.1 1,824,017 - -0.1 1,824,017 - -0.2 1,824,032 + -0.8 1,824,047 - +0.3 1,824,047 - +0.1 1,824,052 - +0.1 1,824,053 - -0.3 1,824,059 - +0.2 1,824,059 - +0.1 1,824,087 + astE b1744 0.11 -0.7 1,824,090 - astE b1744 0.11 -0.3 1,824,090 - astE b1744 0.11 -0.4 1,824,095 - astE b1744 0.12 -0.6 1,824,095 - astE b1744 0.12 +0.5 1,824,095 - astE b1744 0.12 +1.1 1,824,095 - astE b1744 0.12 +0.2 1,824,330 + astE b1744 0.36 +0.7 1,824,333 - astE b1744 0.37 +0.2 1,824,380 + astE b1744 0.41 +0.6 1,824,397 + astE b1744 0.43 -0.6 1,824,512 + astE b1744 0.55 -0.5 1,824,650 - astE b1744 0.69 -0.1 1,824,857 + -0.2 1,824,871 - -0.1 1,825,089 - astB b1745 0.11 +0.4 1,825,285 + astB b1745 0.26 -0.0 1,825,285 + astB b1745 0.26 -0.4 1,825,293 - astB b1745 0.26 -0.3 1,825,321 - astB b1745 0.28 +0.0 1,825,321 - astB b1745 0.28 +0.3 1,825,378 + astB b1745 0.33 +0.5 1,825,727 - astB b1745 0.59 -0.6 1,825,767 - astB b1745 0.62 -0.9
Or see this region's nucleotide sequence