Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 83.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prfB and recJ are separated by 90 nucleotides recJ and dsbC are separated by 5 nucleotides dsbC and xerD are separated by 24 nucleotides
b2891: prfB - peptide chain release factor RF-2 (NCBI), at 3,033,206 to 3,034,304
prfB
b2892: recJ - ssDNA exonuclease, 5' --> 3'-specific (NCBI), at 3,034,395 to 3,036,128
recJ
b2893: dsbC - protein disulfide isomerase II (NCBI), at 3,036,134 to 3,036,844
dsbC
b2894: xerD - site-specific tyrosine recombinase XerD (NCBI), at 3,036,869 to 3,037,765
xerD
Position (kb)
3034
3035
3036
3037 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3034.603 kb on - strand, within recJ at 3034.661 kb on + strand, within recJ at 3034.722 kb on + strand, within recJ at 3034.911 kb on - strand, within recJ at 3034.946 kb on - strand, within recJ at 3035.015 kb on - strand, within recJ at 3035.046 kb on + strand, within recJ at 3035.110 kb on - strand, within recJ at 3035.135 kb on - strand, within recJ at 3035.270 kb on + strand, within recJ at 3035.433 kb on + strand, within recJ at 3035.454 kb on - strand, within recJ at 3035.489 kb on + strand, within recJ at 3035.680 kb on - strand, within recJ at 3035.751 kb on - strand, within recJ at 3035.751 kb on - strand, within recJ at 3035.754 kb on + strand, within recJ at 3035.754 kb on + strand, within recJ at 3035.757 kb on - strand, within recJ at 3035.762 kb on - strand, within recJ at 3035.768 kb on + strand, within recJ at 3035.768 kb on + strand, within recJ at 3035.776 kb on - strand, within recJ at 3035.789 kb on - strand, within recJ at 3035.789 kb on - strand, within recJ at 3035.802 kb on + strand, within recJ at 3035.802 kb on + strand, within recJ at 3035.807 kb on + strand at 3035.815 kb on - strand, within recJ at 3035.854 kb on - strand, within recJ at 3035.854 kb on - strand, within recJ at 3035.864 kb on - strand, within recJ at 3035.869 kb on - strand, within recJ at 3035.906 kb on - strand, within recJ at 3035.906 kb on - strand at 3035.960 kb on - strand at 3035.996 kb on - strand at 3035.996 kb on - strand at 3035.996 kb on - strand at 3036.046 kb on - strand at 3036.068 kb on - strand at 3036.229 kb on + strand, within dsbC at 3036.229 kb on - strand, within dsbC at 3036.268 kb on + strand, within dsbC at 3036.457 kb on - strand, within dsbC at 3036.511 kb on + strand, within dsbC at 3036.511 kb on + strand, within dsbC at 3036.613 kb on - strand, within dsbC at 3036.626 kb on - strand, within dsbC at 3036.660 kb on - strand, within dsbC at 3036.660 kb on - strand, within dsbC at 3036.666 kb on + strand, within dsbC at 3036.668 kb on + strand, within dsbC at 3036.761 kb on + strand, within dsbC
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 83.25 mM remove 3,034,603 - recJ b2892 0.12 +0.6 3,034,661 + recJ b2892 0.15 +0.8 3,034,722 + recJ b2892 0.19 -0.6 3,034,911 - recJ b2892 0.30 +0.7 3,034,946 - recJ b2892 0.32 +0.3 3,035,015 - recJ b2892 0.36 -0.0 3,035,046 + recJ b2892 0.38 -0.8 3,035,110 - recJ b2892 0.41 +0.6 3,035,135 - recJ b2892 0.43 -1.1 3,035,270 + recJ b2892 0.50 +1.2 3,035,433 + recJ b2892 0.60 +0.3 3,035,454 - recJ b2892 0.61 +0.5 3,035,489 + recJ b2892 0.63 +0.4 3,035,680 - recJ b2892 0.74 -1.2 3,035,751 - recJ b2892 0.78 -2.5 3,035,751 - recJ b2892 0.78 +0.1 3,035,754 + recJ b2892 0.78 +0.2 3,035,754 + recJ b2892 0.78 -0.7 3,035,757 - recJ b2892 0.79 -0.2 3,035,762 - recJ b2892 0.79 -0.8 3,035,768 + recJ b2892 0.79 +0.6 3,035,768 + recJ b2892 0.79 -0.4 3,035,776 - recJ b2892 0.80 +0.4 3,035,789 - recJ b2892 0.80 +0.7 3,035,789 - recJ b2892 0.80 -0.5 3,035,802 + recJ b2892 0.81 +0.0 3,035,802 + recJ b2892 0.81 -0.5 3,035,807 + -2.4 3,035,815 - recJ b2892 0.82 +0.7 3,035,854 - recJ b2892 0.84 +0.6 3,035,854 - recJ b2892 0.84 -3.6 3,035,864 - recJ b2892 0.85 -0.1 3,035,869 - recJ b2892 0.85 -0.3 3,035,906 - recJ b2892 0.87 +0.4 3,035,906 - -1.4 3,035,960 - +0.3 3,035,996 - -2.4 3,035,996 - +0.9 3,035,996 - +0.3 3,036,046 - +1.6 3,036,068 - -0.3 3,036,229 + dsbC b2893 0.13 -0.0 3,036,229 - dsbC b2893 0.13 -0.4 3,036,268 + dsbC b2893 0.19 -0.1 3,036,457 - dsbC b2893 0.45 -0.5 3,036,511 + dsbC b2893 0.53 +0.7 3,036,511 + dsbC b2893 0.53 +0.0 3,036,613 - dsbC b2893 0.67 -0.4 3,036,626 - dsbC b2893 0.69 -0.1 3,036,660 - dsbC b2893 0.74 -0.6 3,036,660 - dsbC b2893 0.74 -0.1 3,036,666 + dsbC b2893 0.75 +0.3 3,036,668 + dsbC b2893 0.75 -0.3 3,036,761 + dsbC b2893 0.88 -0.4
Or see this region's nucleotide sequence