Strain Fitness in Escherichia coli BW25113 around b4180

Experiment: 1/10ON stiff

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntvacB and rlmB are separated by 179 nucleotidesrlmB and yjfI are separated by 126 nucleotidesyjfI and yjfJ are separated by 18 nucleotides b4179: vacB - putative enzyme (VIMSS), at 4,404,677 to 4,407,118 vacB b4180: rlmB - 23S rRNA (Gm2251)-methyltransferase (NCBI), at 4,407,298 to 4,408,029 rlmB b4181: yjfI - hypothetical protein (NCBI), at 4,408,156 to 4,408,557 yjfI b4182: yjfJ - predicted transcriptional regulator effector protein (NCBI), at 4,408,576 to 4,409,274 yjfJ Position (kb) 4407 4408 4409Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4406.330 kb on - strand, within vacBat 4406.455 kb on + strand, within vacBat 4406.455 kb on + strand, within vacBat 4406.465 kb on + strand, within vacBat 4406.465 kb on + strand, within vacBat 4406.471 kb on - strand, within vacBat 4406.516 kb on - strand, within vacBat 4406.518 kb on + strand, within vacBat 4406.523 kb on + strand, within vacBat 4406.526 kb on - strand, within vacBat 4406.529 kb on - strand, within vacBat 4406.564 kb on + strand, within vacBat 4406.727 kb on - strand, within vacBat 4406.727 kb on - strand, within vacBat 4406.743 kb on - strand, within vacBat 4406.812 kb on + strand, within vacBat 4406.846 kb on - strand, within vacBat 4406.846 kb on - strand, within vacBat 4407.253 kb on + strandat 4407.286 kb on - strandat 4407.328 kb on + strandat 4407.335 kb on + strandat 4407.335 kb on + strandat 4407.336 kb on - strandat 4407.336 kb on - strandat 4407.336 kb on - strandat 4407.336 kb on - strandat 4407.345 kb on - strandat 4407.345 kb on - strandat 4407.346 kb on - strandat 4407.404 kb on + strand, within rlmBat 4407.404 kb on + strand, within rlmBat 4407.501 kb on + strand, within rlmBat 4407.534 kb on - strand, within rlmBat 4407.534 kb on - strandat 4407.704 kb on - strand, within rlmBat 4407.772 kb on - strand, within rlmBat 4407.772 kb on - strand, within rlmBat 4407.776 kb on + strand, within rlmBat 4407.776 kb on + strand, within rlmBat 4407.833 kb on + strand, within rlmBat 4407.842 kb on - strand, within rlmBat 4407.849 kb on - strand, within rlmBat 4407.874 kb on + strand, within rlmBat 4407.876 kb on + strand, within rlmBat 4407.876 kb on + strand, within rlmBat 4407.882 kb on - strand, within rlmBat 4407.889 kb on + strand, within rlmBat 4407.891 kb on - strand, within rlmBat 4407.898 kb on + strand, within rlmBat 4407.906 kb on + strand, within rlmBat 4407.907 kb on + strand, within rlmBat 4407.924 kb on - strand, within rlmBat 4407.941 kb on + strand, within rlmBat 4407.942 kb on - strand, within rlmBat 4407.942 kb on - strand, within rlmBat 4407.951 kb on + strand, within rlmBat 4407.953 kb on - strand, within rlmBat 4407.967 kb on + strandat 4407.967 kb on + strandat 4407.977 kb on + strandat 4408.012 kb on + strandat 4408.012 kb on + strandat 4408.012 kb on - strandat 4408.147 kb on + strandat 4408.152 kb on + strandat 4408.152 kb on + strandat 4408.152 kb on + strandat 4408.179 kb on + strandat 4408.186 kb on + strandat 4408.189 kb on + strandat 4408.193 kb on + strandat 4408.193 kb on + strandat 4408.325 kb on - strand, within yjfIat 4408.337 kb on - strand, within yjfIat 4408.344 kb on - strand, within yjfIat 4408.344 kb on - strand, within yjfIat 4408.344 kb on - strand, within yjfIat 4408.344 kb on - strand, within yjfIat 4408.345 kb on - strand, within yjfIat 4408.406 kb on - strand, within yjfIat 4408.406 kb on - strand, within yjfIat 4408.411 kb on - strand, within yjfIat 4408.413 kb on - strand, within yjfIat 4408.413 kb on - strand, within yjfIat 4408.413 kb on - strand, within yjfIat 4408.413 kb on - strand, within yjfIat 4408.413 kb on - strand, within yjfIat 4408.423 kb on - strand, within yjfIat 4408.425 kb on - strand, within yjfIat 4408.442 kb on - strand, within yjfIat 4408.469 kb on - strand, within yjfIat 4408.500 kb on - strand, within yjfIat 4408.500 kb on - strand, within yjfIat 4408.512 kb on - strand, within yjfIat 4408.638 kb on - strandat 4408.662 kb on - strand, within yjfJat 4408.662 kb on - strand, within yjfJat 4408.666 kb on + strand, within yjfJat 4408.666 kb on + strand, within yjfJat 4408.668 kb on - strand, within yjfJat 4408.668 kb on - strand, within yjfJat 4408.670 kb on - strand, within yjfJat 4408.672 kb on - strand, within yjfJat 4408.672 kb on - strand, within yjfJat 4408.694 kb on - strand, within yjfJat 4408.694 kb on - strand, within yjfJat 4408.777 kb on - strand, within yjfJat 4408.779 kb on + strand, within yjfJat 4408.779 kb on + strand, within yjfJat 4408.788 kb on - strand, within yjfJat 4408.807 kb on + strand, within yjfJat 4408.813 kb on - strand, within yjfJat 4408.879 kb on - strand, within yjfJat 4408.894 kb on + strand, within yjfJat 4408.894 kb on + strand, within yjfJat 4408.897 kb on + strand, within yjfJat 4408.897 kb on + strand, within yjfJat 4408.931 kb on - strand, within yjfJat 4408.960 kb on - strand, within yjfJat 4408.986 kb on + strand, within yjfJat 4409.015 kb on - strand, within yjfJat 4409.022 kb on - strand, within yjfJ

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Per-strain Table

Position Strand Gene LocusTag Fraction 1/10ON stiff
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4,406,330 - vacB b4179 0.68 -1.7
4,406,455 + vacB b4179 0.73 -1.1
4,406,455 + vacB b4179 0.73 -1.2
4,406,465 + vacB b4179 0.73 -0.5
4,406,465 + vacB b4179 0.73 +0.9
4,406,471 - vacB b4179 0.73 -0.1
4,406,516 - vacB b4179 0.75 +0.9
4,406,518 + vacB b4179 0.75 -1.7
4,406,523 + vacB b4179 0.76 -1.1
4,406,526 - vacB b4179 0.76 -0.1
4,406,529 - vacB b4179 0.76 -0.1
4,406,564 + vacB b4179 0.77 +1.2
4,406,727 - vacB b4179 0.84 -1.1
4,406,727 - vacB b4179 0.84 -1.7
4,406,743 - vacB b4179 0.85 +0.9
4,406,812 + vacB b4179 0.87 +1.5
4,406,846 - vacB b4179 0.89 -0.8
4,406,846 - vacB b4179 0.89 +0.3
4,407,253 + +0.9
4,407,286 - -0.7
4,407,328 + -0.3
4,407,335 + +0.7
4,407,335 + -0.3
4,407,336 - -1.5
4,407,336 - +1.2
4,407,336 - -0.5
4,407,336 - -1.7
4,407,345 - +0.7
4,407,345 - -1.4
4,407,346 - -0.1
4,407,404 + rlmB b4180 0.14 -2.4
4,407,404 + rlmB b4180 0.14 +0.1
4,407,501 + rlmB b4180 0.28 -1.7
4,407,534 - rlmB b4180 0.32 +0.7
4,407,534 - -0.1
4,407,704 - rlmB b4180 0.55 -2.9
4,407,772 - rlmB b4180 0.65 -0.1
4,407,772 - rlmB b4180 0.65 -1.7
4,407,776 + rlmB b4180 0.65 -0.8
4,407,776 + rlmB b4180 0.65 -0.1
4,407,833 + rlmB b4180 0.73 -2.3
4,407,842 - rlmB b4180 0.74 -0.9
4,407,849 - rlmB b4180 0.75 -0.1
4,407,874 + rlmB b4180 0.79 -2.7
4,407,876 + rlmB b4180 0.79 -0.5
4,407,876 + rlmB b4180 0.79 -1.1
4,407,882 - rlmB b4180 0.80 +0.8
4,407,889 + rlmB b4180 0.81 +0.5
4,407,891 - rlmB b4180 0.81 -0.6
4,407,898 + rlmB b4180 0.82 -0.7
4,407,906 + rlmB b4180 0.83 -0.7
4,407,907 + rlmB b4180 0.83 -0.5
4,407,924 - rlmB b4180 0.86 +2.2
4,407,941 + rlmB b4180 0.88 +0.2
4,407,942 - rlmB b4180 0.88 -0.1
4,407,942 - rlmB b4180 0.88 -0.1
4,407,951 + rlmB b4180 0.89 -2.1
4,407,953 - rlmB b4180 0.89 -0.1
4,407,967 + -1.1
4,407,967 + -1.3
4,407,977 + -1.0
4,408,012 + -0.0
4,408,012 + -0.3
4,408,012 - -0.1
4,408,147 + -0.1
4,408,152 + -0.1
4,408,152 + -1.7
4,408,152 + -1.1
4,408,179 + -0.1
4,408,186 + -0.1
4,408,189 + -0.1
4,408,193 + -0.1
4,408,193 + -0.1
4,408,325 - yjfI b4181 0.42 -0.1
4,408,337 - yjfI b4181 0.45 -0.1
4,408,344 - yjfI b4181 0.47 -0.1
4,408,344 - yjfI b4181 0.47 +0.5
4,408,344 - yjfI b4181 0.47 -1.1
4,408,344 - yjfI b4181 0.47 -1.7
4,408,345 - yjfI b4181 0.47 -1.1
4,408,406 - yjfI b4181 0.62 -0.1
4,408,406 - yjfI b4181 0.62 -0.1
4,408,411 - yjfI b4181 0.63 -1.7
4,408,413 - yjfI b4181 0.64 -0.1
4,408,413 - yjfI b4181 0.64 -0.1
4,408,413 - yjfI b4181 0.64 -1.7
4,408,413 - yjfI b4181 0.64 +0.5
4,408,413 - yjfI b4181 0.64 +1.5
4,408,423 - yjfI b4181 0.66 -0.1
4,408,425 - yjfI b4181 0.67 -0.4
4,408,442 - yjfI b4181 0.71 -0.1
4,408,469 - yjfI b4181 0.78 +1.8
4,408,500 - yjfI b4181 0.86 -0.1
4,408,500 - yjfI b4181 0.86 -0.1
4,408,512 - yjfI b4181 0.89 -0.1
4,408,638 - -1.4
4,408,662 - yjfJ b4182 0.12 +0.9
4,408,662 - yjfJ b4182 0.12 -0.1
4,408,666 + yjfJ b4182 0.13 -0.1
4,408,666 + yjfJ b4182 0.13 -0.1
4,408,668 - yjfJ b4182 0.13 +1.9
4,408,668 - yjfJ b4182 0.13 -1.7
4,408,670 - yjfJ b4182 0.13 +0.2
4,408,672 - yjfJ b4182 0.14 +0.4
4,408,672 - yjfJ b4182 0.14 -0.5
4,408,694 - yjfJ b4182 0.17 +0.7
4,408,694 - yjfJ b4182 0.17 +0.5
4,408,777 - yjfJ b4182 0.29 -0.1
4,408,779 + yjfJ b4182 0.29 +2.5
4,408,779 + yjfJ b4182 0.29 +0.3
4,408,788 - yjfJ b4182 0.30 -0.4
4,408,807 + yjfJ b4182 0.33 +0.2
4,408,813 - yjfJ b4182 0.34 +0.4
4,408,879 - yjfJ b4182 0.43 +1.0
4,408,894 + yjfJ b4182 0.45 +0.4
4,408,894 + yjfJ b4182 0.45 +0.4
4,408,897 + yjfJ b4182 0.46 -0.3
4,408,897 + yjfJ b4182 0.46 -0.1
4,408,931 - yjfJ b4182 0.51 +0.6
4,408,960 - yjfJ b4182 0.55 +0.4
4,408,986 + yjfJ b4182 0.59 +0.2
4,409,015 - yjfJ b4182 0.63 +0.3
4,409,022 - yjfJ b4182 0.64 +0.7

Or see this region's nucleotide sequence