Experiment: 1/10ON stiff
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fucK and fucU are separated by 1 nucleotides fucU and fucR are separated by 57 nucleotides fucR and ygdE are separated by 43 nucleotides
b2803: fucK - L-fuculokinase (NCBI), at 2,935,460 to 2,936,908
fucK
b2804: fucU - L-fucose mutarotase (NCBI), at 2,936,910 to 2,937,332
fucU
b2805: fucR - DNA-binding transcriptional activator (NCBI), at 2,937,390 to 2,938,121
fucR
b2806: ygdE - predicted methyltransferase (NCBI), at 2,938,165 to 2,939,265
ygdE
Position (kb)
2937
2938
2939 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2936.392 kb on - strand, within fucK at 2936.441 kb on + strand, within fucK at 2936.538 kb on + strand, within fucK at 2936.538 kb on + strand, within fucK at 2936.628 kb on + strand, within fucK at 2936.630 kb on + strand, within fucK at 2936.630 kb on + strand, within fucK at 2936.656 kb on + strand, within fucK at 2936.656 kb on + strand, within fucK at 2936.664 kb on - strand, within fucK at 2936.664 kb on - strand, within fucK at 2936.808 kb on - strand at 2936.845 kb on + strand at 2936.845 kb on + strand at 2936.974 kb on - strand, within fucU at 2936.974 kb on - strand, within fucU at 2937.103 kb on + strand, within fucU at 2937.103 kb on + strand, within fucU at 2937.173 kb on + strand, within fucU at 2937.434 kb on - strand at 2937.434 kb on - strand at 2937.439 kb on - strand at 2937.473 kb on - strand, within fucR at 2937.629 kb on + strand, within fucR at 2937.677 kb on - strand, within fucR at 2937.677 kb on - strand, within fucR at 2937.681 kb on + strand, within fucR at 2937.747 kb on - strand, within fucR at 2937.769 kb on + strand, within fucR at 2937.769 kb on + strand, within fucR at 2937.811 kb on - strand, within fucR at 2937.811 kb on - strand, within fucR at 2937.817 kb on + strand, within fucR at 2937.862 kb on + strand, within fucR at 2938.088 kb on + strand at 2938.132 kb on - strand at 2938.204 kb on - strand at 2938.353 kb on - strand, within ygdE at 2938.353 kb on - strand, within ygdE at 2938.398 kb on - strand, within ygdE at 2938.443 kb on + strand, within ygdE at 2938.443 kb on - strand, within ygdE at 2938.553 kb on + strand, within ygdE at 2938.561 kb on - strand, within ygdE at 2938.561 kb on - strand, within ygdE at 2938.587 kb on + strand, within ygdE at 2938.610 kb on + strand, within ygdE at 2938.698 kb on + strand, within ygdE at 2938.711 kb on - strand, within ygdE at 2938.711 kb on - strand, within ygdE at 2938.750 kb on - strand, within ygdE at 2938.758 kb on + strand, within ygdE at 2938.780 kb on - strand, within ygdE at 2938.780 kb on - strand, within ygdE at 2938.795 kb on - strand, within ygdE at 2938.795 kb on - strand, within ygdE at 2938.810 kb on + strand, within ygdE at 2938.810 kb on + strand, within ygdE at 2938.868 kb on - strand, within ygdE at 2938.883 kb on - strand, within ygdE at 2938.969 kb on + strand, within ygdE at 2938.974 kb on + strand, within ygdE at 2938.974 kb on + strand, within ygdE at 2938.986 kb on + strand, within ygdE at 2939.038 kb on - strand, within ygdE
Per-strain Table
Position Strand Gene LocusTag Fraction 1/10ON stiff remove 2,936,392 - fucK b2803 0.64 +0.1 2,936,441 + fucK b2803 0.68 +0.9 2,936,538 + fucK b2803 0.74 +0.1 2,936,538 + fucK b2803 0.74 +0.9 2,936,628 + fucK b2803 0.81 -0.2 2,936,630 + fucK b2803 0.81 +1.3 2,936,630 + fucK b2803 0.81 +1.3 2,936,656 + fucK b2803 0.83 -0.3 2,936,656 + fucK b2803 0.83 -0.6 2,936,664 - fucK b2803 0.83 -0.1 2,936,664 - fucK b2803 0.83 +0.5 2,936,808 - +0.5 2,936,845 + -0.7 2,936,845 + +0.8 2,936,974 - fucU b2804 0.15 -0.6 2,936,974 - fucU b2804 0.15 +0.2 2,937,103 + fucU b2804 0.46 +0.3 2,937,103 + fucU b2804 0.46 -0.1 2,937,173 + fucU b2804 0.62 +0.3 2,937,434 - -0.2 2,937,434 - +1.5 2,937,439 - +0.1 2,937,473 - fucR b2805 0.11 -1.6 2,937,629 + fucR b2805 0.33 -1.2 2,937,677 - fucR b2805 0.39 +0.4 2,937,677 - fucR b2805 0.39 -0.1 2,937,681 + fucR b2805 0.40 -1.5 2,937,747 - fucR b2805 0.49 +0.5 2,937,769 + fucR b2805 0.52 +0.4 2,937,769 + fucR b2805 0.52 -1.3 2,937,811 - fucR b2805 0.58 +0.2 2,937,811 - fucR b2805 0.58 +0.5 2,937,817 + fucR b2805 0.58 -0.2 2,937,862 + fucR b2805 0.64 -0.6 2,938,088 + +0.3 2,938,132 - +0.9 2,938,204 - -1.1 2,938,353 - ygdE b2806 0.17 +0.3 2,938,353 - ygdE b2806 0.17 +0.8 2,938,398 - ygdE b2806 0.21 -0.1 2,938,443 + ygdE b2806 0.25 -1.1 2,938,443 - ygdE b2806 0.25 +0.7 2,938,553 + ygdE b2806 0.35 +0.7 2,938,561 - ygdE b2806 0.36 -0.1 2,938,561 - ygdE b2806 0.36 +0.0 2,938,587 + ygdE b2806 0.38 -2.9 2,938,610 + ygdE b2806 0.40 -2.2 2,938,698 + ygdE b2806 0.48 -0.3 2,938,711 - ygdE b2806 0.50 +0.4 2,938,711 - ygdE b2806 0.50 -1.2 2,938,750 - ygdE b2806 0.53 -0.4 2,938,758 + ygdE b2806 0.54 -0.9 2,938,780 - ygdE b2806 0.56 +0.7 2,938,780 - ygdE b2806 0.56 +0.2 2,938,795 - ygdE b2806 0.57 +0.3 2,938,795 - ygdE b2806 0.57 +0.6 2,938,810 + ygdE b2806 0.59 -0.9 2,938,810 + ygdE b2806 0.59 +0.7 2,938,868 - ygdE b2806 0.64 -0.3 2,938,883 - ygdE b2806 0.65 -0.6 2,938,969 + ygdE b2806 0.73 +0.7 2,938,974 + ygdE b2806 0.73 -0.7 2,938,974 + ygdE b2806 0.73 -1.9 2,938,986 + ygdE b2806 0.75 +0.4 2,939,038 - ygdE b2806 0.79 -0.2
Or see this region's nucleotide sequence