Experiment: 1/10ON stiff
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygaY and ygaZ are separated by 123 nucleotides ygaZ and ygaH overlap by 11 nucleotides ygaH and mprA are separated by 90 nucleotides
b2681: ygaY - predicted transporter (pseudogene) (RefSeq), at 2,806,338 to 2,807,515
ygaY
b2682: ygaZ - predicted transporter (NCBI), at 2,807,639 to 2,808,376
ygaZ
b2683: ygaH - predicted inner membrane protein (NCBI), at 2,808,366 to 2,808,701
ygaH
b2684: mprA - DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI), at 2,808,792 to 2,809,322
mprA
Position (kb)
2807
2808
2809 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2806.745 kb on + strand, within ygaY at 2806.753 kb on - strand, within ygaY at 2806.753 kb on - strand, within ygaY at 2806.759 kb on + strand, within ygaY at 2806.759 kb on + strand, within ygaY at 2806.760 kb on + strand, within ygaY at 2806.870 kb on + strand, within ygaY at 2806.870 kb on + strand, within ygaY at 2806.876 kb on - strand, within ygaY at 2806.876 kb on - strand, within ygaY at 2806.925 kb on - strand, within ygaY at 2807.086 kb on + strand, within ygaY at 2807.111 kb on - strand, within ygaY at 2807.111 kb on - strand, within ygaY at 2807.327 kb on - strand, within ygaY at 2807.604 kb on + strand at 2807.646 kb on + strand at 2807.646 kb on + strand at 2807.853 kb on - strand, within ygaZ at 2807.917 kb on - strand, within ygaZ at 2807.917 kb on - strand, within ygaZ at 2807.919 kb on - strand, within ygaZ at 2808.115 kb on - strand, within ygaZ at 2808.115 kb on - strand, within ygaZ at 2808.190 kb on + strand, within ygaZ at 2808.190 kb on + strand, within ygaZ at 2808.198 kb on - strand, within ygaZ at 2808.322 kb on - strand at 2808.322 kb on - strand at 2808.379 kb on - strand at 2808.379 kb on - strand at 2808.399 kb on - strand at 2808.515 kb on + strand, within ygaH at 2808.517 kb on - strand, within ygaH at 2808.533 kb on + strand, within ygaH at 2808.533 kb on + strand, within ygaH at 2808.534 kb on - strand, within ygaH at 2808.534 kb on - strand, within ygaH at 2808.560 kb on - strand, within ygaH at 2808.565 kb on - strand at 2808.645 kb on + strand, within ygaH at 2808.645 kb on + strand, within ygaH at 2808.645 kb on + strand, within ygaH at 2808.648 kb on - strand, within ygaH at 2808.655 kb on + strand, within ygaH at 2808.658 kb on - strand, within ygaH at 2808.660 kb on - strand, within ygaH at 2808.662 kb on + strand, within ygaH at 2808.662 kb on + strand, within ygaH at 2808.671 kb on - strand at 2808.876 kb on - strand, within mprA at 2809.100 kb on + strand, within mprA at 2809.100 kb on + strand, within mprA at 2809.107 kb on + strand, within mprA at 2809.200 kb on + strand, within mprA at 2809.287 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1/10ON stiff remove 2,806,745 + ygaY b2681 0.35 -0.0 2,806,753 - ygaY b2681 0.35 +0.7 2,806,753 - ygaY b2681 0.35 +0.0 2,806,759 + ygaY b2681 0.36 +0.6 2,806,759 + ygaY b2681 0.36 +0.7 2,806,760 + ygaY b2681 0.36 +0.6 2,806,870 + ygaY b2681 0.45 +0.1 2,806,870 + ygaY b2681 0.45 +0.5 2,806,876 - ygaY b2681 0.46 +0.3 2,806,876 - ygaY b2681 0.46 +0.5 2,806,925 - ygaY b2681 0.50 +0.2 2,807,086 + ygaY b2681 0.63 +0.1 2,807,111 - ygaY b2681 0.66 -1.0 2,807,111 - ygaY b2681 0.66 +1.4 2,807,327 - ygaY b2681 0.84 +1.0 2,807,604 + -0.1 2,807,646 + +0.1 2,807,646 + +0.4 2,807,853 - ygaZ b2682 0.29 +0.2 2,807,917 - ygaZ b2682 0.38 -0.5 2,807,917 - ygaZ b2682 0.38 -1.1 2,807,919 - ygaZ b2682 0.38 +0.7 2,808,115 - ygaZ b2682 0.64 -1.3 2,808,115 - ygaZ b2682 0.64 -1.1 2,808,190 + ygaZ b2682 0.75 -1.6 2,808,190 + ygaZ b2682 0.75 +0.5 2,808,198 - ygaZ b2682 0.76 -1.1 2,808,322 - +0.3 2,808,322 - +0.3 2,808,379 - -0.6 2,808,379 - +0.5 2,808,399 - -0.2 2,808,515 + ygaH b2683 0.44 -0.4 2,808,517 - ygaH b2683 0.45 +2.5 2,808,533 + ygaH b2683 0.50 +0.6 2,808,533 + ygaH b2683 0.50 -1.2 2,808,534 - ygaH b2683 0.50 +1.7 2,808,534 - ygaH b2683 0.50 +1.1 2,808,560 - ygaH b2683 0.58 -0.3 2,808,565 - -0.0 2,808,645 + ygaH b2683 0.83 -4.0 2,808,645 + ygaH b2683 0.83 -1.3 2,808,645 + ygaH b2683 0.83 -2.4 2,808,648 - ygaH b2683 0.84 +0.7 2,808,655 + ygaH b2683 0.86 -1.0 2,808,658 - ygaH b2683 0.87 +0.8 2,808,660 - ygaH b2683 0.88 -0.9 2,808,662 + ygaH b2683 0.88 -2.5 2,808,662 + ygaH b2683 0.88 -0.9 2,808,671 - +0.5 2,808,876 - mprA b2684 0.16 -0.0 2,809,100 + mprA b2684 0.58 +1.2 2,809,100 + mprA b2684 0.58 +1.7 2,809,107 + mprA b2684 0.59 +1.4 2,809,200 + mprA b2684 0.77 +0.7 2,809,287 + +0.9
Or see this region's nucleotide sequence