Strain Fitness in Escherichia coli BW25113 around b1856

Experiment: 1/10ON stiff

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlpxM and yebA are separated by 119 nucleotidesyebA and yebL are separated by 15 nucleotides b1855: lpxM - lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI), at 1,937,246 to 1,938,217 lpxM b1856: yebA - orf, hypothetical protein (VIMSS), at 1,938,337 to 1,939,659 yebA b1857: yebL - putative adhesin (VIMSS), at 1,939,675 to 1,940,607 yebL Position (kb) 1938 1939 1940Strain fitness (log2 ratio) -2 -1 0 1 2at 1937.580 kb on - strand, within lpxMat 1937.674 kb on + strand, within lpxMat 1937.696 kb on + strand, within lpxMat 1937.696 kb on + strand, within lpxMat 1937.738 kb on - strand, within lpxMat 1937.742 kb on + strand, within lpxMat 1937.844 kb on + strand, within lpxMat 1937.989 kb on + strand, within lpxMat 1937.997 kb on + strand, within lpxMat 1938.027 kb on - strand, within lpxMat 1938.132 kb on - strandat 1938.137 kb on - strandat 1938.167 kb on + strandat 1938.546 kb on + strand, within yebAat 1938.626 kb on - strand, within yebAat 1938.668 kb on + strand, within yebAat 1938.726 kb on - strand, within yebAat 1938.822 kb on + strand, within yebAat 1938.822 kb on + strand, within yebAat 1939.004 kb on - strand, within yebAat 1939.128 kb on - strand, within yebAat 1939.179 kb on - strand, within yebAat 1939.204 kb on - strand, within yebAat 1939.241 kb on - strand, within yebAat 1939.243 kb on - strand, within yebAat 1939.287 kb on + strand, within yebAat 1939.301 kb on + strand, within yebAat 1939.301 kb on + strand, within yebAat 1939.423 kb on + strand, within yebAat 1939.440 kb on - strand, within yebAat 1939.476 kb on - strand, within yebAat 1939.652 kb on - strandat 1939.694 kb on + strandat 1939.813 kb on + strand, within yebLat 1939.826 kb on + strand, within yebLat 1939.826 kb on + strand, within yebLat 1939.834 kb on - strand, within yebLat 1939.834 kb on - strand, within yebLat 1939.834 kb on - strand, within yebLat 1940.017 kb on - strand, within yebLat 1940.017 kb on - strand, within yebLat 1940.087 kb on - strand, within yebLat 1940.087 kb on - strand, within yebLat 1940.238 kb on - strand, within yebLat 1940.238 kb on - strand, within yebLat 1940.554 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1/10ON stiff
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1,937,580 - lpxM b1855 0.34 +0.1
1,937,674 + lpxM b1855 0.44 -0.2
1,937,696 + lpxM b1855 0.46 -1.0
1,937,696 + lpxM b1855 0.46 +1.1
1,937,738 - lpxM b1855 0.51 +1.1
1,937,742 + lpxM b1855 0.51 +1.0
1,937,844 + lpxM b1855 0.62 -0.1
1,937,989 + lpxM b1855 0.76 +0.1
1,937,997 + lpxM b1855 0.77 +0.4
1,938,027 - lpxM b1855 0.80 +0.1
1,938,132 - +1.0
1,938,137 - +0.5
1,938,167 + -0.9
1,938,546 + yebA b1856 0.16 +1.1
1,938,626 - yebA b1856 0.22 -0.3
1,938,668 + yebA b1856 0.25 +1.3
1,938,726 - yebA b1856 0.29 +0.4
1,938,822 + yebA b1856 0.37 -0.1
1,938,822 + yebA b1856 0.37 +0.4
1,939,004 - yebA b1856 0.50 -0.5
1,939,128 - yebA b1856 0.60 -1.0
1,939,179 - yebA b1856 0.64 +0.2
1,939,204 - yebA b1856 0.66 +0.1
1,939,241 - yebA b1856 0.68 +1.1
1,939,243 - yebA b1856 0.68 +1.2
1,939,287 + yebA b1856 0.72 -1.4
1,939,301 + yebA b1856 0.73 -0.5
1,939,301 + yebA b1856 0.73 +0.6
1,939,423 + yebA b1856 0.82 -0.2
1,939,440 - yebA b1856 0.83 -1.9
1,939,476 - yebA b1856 0.86 +0.6
1,939,652 - +0.5
1,939,694 + -1.4
1,939,813 + yebL b1857 0.15 -0.8
1,939,826 + yebL b1857 0.16 -0.7
1,939,826 + yebL b1857 0.16 +0.5
1,939,834 - yebL b1857 0.17 +0.3
1,939,834 - yebL b1857 0.17 +1.8
1,939,834 - yebL b1857 0.17 +0.4
1,940,017 - yebL b1857 0.37 +0.6
1,940,017 - yebL b1857 0.37 -1.1
1,940,087 - yebL b1857 0.44 +1.1
1,940,087 - yebL b1857 0.44 +2.0
1,940,238 - yebL b1857 0.60 +0.3
1,940,238 - yebL b1857 0.60 -0.9
1,940,554 + +0.3

Or see this region's nucleotide sequence