Experiment: 1/10ON stiff
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ahpF and uspG are separated by 120 nucleotides uspG and ybdR are separated by 220 nucleotides ybdR and rnk are separated by 230 nucleotides rnk and rna are separated by 229 nucleotides
b0606: ahpF - alkyl hydroperoxide reductase, F52a subunit; detoxification of hydroperoxides (VIMSS), at 638,976 to 640,541
ahpF
b0607: uspG - universal stress protein UP12 (NCBI), at 640,662 to 641,090
uspG
b0608: ybdR - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI), at 641,311 to 642,549
ybdR
b0610: rnk - nucleoside diphosphate kinase regulator (NCBI), at 642,780 to 643,190
rnk
b0611: rna - ribonuclease I (NCBI), at 643,420 to 644,226
rna
Position (kb) 641
642
643 Strain fitness (log2 ratio) -2
-1
0
1
2 at 640.314 kb on + strand, within ahpF at 640.364 kb on + strand, within ahpF at 640.391 kb on - strand at 640.391 kb on - strand at 640.447 kb on - strand at 640.486 kb on + strand at 640.486 kb on + strand at 640.605 kb on + strand at 640.605 kb on + strand at 640.637 kb on - strand at 640.637 kb on - strand at 640.642 kb on - strand at 640.647 kb on - strand at 640.647 kb on - strand at 640.670 kb on + strand at 640.670 kb on + strand at 640.782 kb on - strand, within uspG at 640.787 kb on - strand, within uspG at 640.787 kb on - strand, within uspG at 640.808 kb on - strand, within uspG at 640.813 kb on - strand, within uspG at 640.824 kb on - strand, within uspG at 640.863 kb on - strand, within uspG at 640.863 kb on - strand, within uspG at 640.887 kb on + strand, within uspG at 640.889 kb on + strand, within uspG at 640.889 kb on + strand, within uspG at 640.949 kb on + strand, within uspG at 640.949 kb on + strand, within uspG at 640.958 kb on + strand, within uspG at 640.976 kb on + strand, within uspG at 640.996 kb on - strand, within uspG at 641.001 kb on + strand, within uspG at 641.001 kb on + strand, within uspG at 641.075 kb on + strand at 641.080 kb on + strand at 641.080 kb on + strand at 641.080 kb on + strand at 641.080 kb on + strand at 641.088 kb on - strand at 641.088 kb on - strand at 641.139 kb on - strand at 641.221 kb on - strand at 641.221 kb on - strand at 641.223 kb on + strand at 641.266 kb on - strand at 641.266 kb on - strand at 641.286 kb on + strand at 641.286 kb on + strand at 641.342 kb on + strand at 641.342 kb on + strand at 641.372 kb on + strand at 641.372 kb on + strand at 641.434 kb on - strand at 641.434 kb on - strand at 641.576 kb on + strand, within ybdR at 641.576 kb on + strand, within ybdR at 641.586 kb on + strand, within ybdR at 641.646 kb on - strand, within ybdR at 641.655 kb on + strand, within ybdR at 641.655 kb on + strand, within ybdR at 641.734 kb on + strand, within ybdR at 641.891 kb on - strand, within ybdR at 641.900 kb on - strand, within ybdR at 642.079 kb on + strand, within ybdR at 642.087 kb on - strand, within ybdR at 642.097 kb on - strand, within ybdR at 642.173 kb on + strand, within ybdR at 642.191 kb on + strand, within ybdR at 642.218 kb on + strand, within ybdR at 642.317 kb on - strand, within ybdR at 642.412 kb on + strand, within ybdR at 642.665 kb on + strand at 642.665 kb on + strand at 642.747 kb on - strand at 642.750 kb on - strand at 642.750 kb on - strand at 642.784 kb on + strand at 642.785 kb on - strand at 642.890 kb on + strand, within rnk at 642.922 kb on + strand, within rnk at 643.290 kb on - strand at 643.339 kb on + strand at 643.347 kb on - strand at 643.530 kb on + strand, within rna
Per-strain Table
Position Strand Gene LocusTag Fraction 1/10ON stiff remove 640,314 + ahpF b0606 0.85 -2.1 640,364 + ahpF b0606 0.89 -0.8 640,391 - -0.7 640,391 - -0.3 640,447 - -0.3 640,486 + -0.2 640,486 + -1.3 640,605 + -0.4 640,605 + +1.8 640,637 - -1.3 640,637 - -0.0 640,642 - +1.8 640,647 - +1.0 640,647 - -0.9 640,670 + +0.1 640,670 + +1.0 640,782 - uspG b0607 0.28 +0.6 640,787 - uspG b0607 0.29 +1.4 640,787 - uspG b0607 0.29 -0.0 640,808 - uspG b0607 0.34 +0.4 640,813 - uspG b0607 0.35 +1.0 640,824 - uspG b0607 0.38 +0.5 640,863 - uspG b0607 0.47 +0.2 640,863 - uspG b0607 0.47 +0.1 640,887 + uspG b0607 0.52 -0.2 640,889 + uspG b0607 0.53 +0.2 640,889 + uspG b0607 0.53 -0.5 640,949 + uspG b0607 0.67 -1.0 640,949 + uspG b0607 0.67 -0.0 640,958 + uspG b0607 0.69 +0.6 640,976 + uspG b0607 0.73 -0.8 640,996 - uspG b0607 0.78 +0.2 641,001 + uspG b0607 0.79 -0.0 641,001 + uspG b0607 0.79 -1.6 641,075 + -0.0 641,080 + +1.0 641,080 + -1.0 641,080 + -0.2 641,080 + -1.6 641,088 - +0.1 641,088 - +0.1 641,139 - -0.7 641,221 - -0.4 641,221 - +0.3 641,223 + -1.0 641,266 - +0.2 641,266 - +1.1 641,286 + +0.9 641,286 + +1.6 641,342 + -0.3 641,342 + +1.4 641,372 + +2.6 641,372 + -1.6 641,434 - -0.3 641,434 - -0.2 641,576 + ybdR b0608 0.21 -0.2 641,576 + ybdR b0608 0.21 -0.2 641,586 + ybdR b0608 0.22 +1.6 641,646 - ybdR b0608 0.27 -0.2 641,655 + ybdR b0608 0.28 -0.7 641,655 + ybdR b0608 0.28 -1.0 641,734 + ybdR b0608 0.34 +0.4 641,891 - ybdR b0608 0.47 +0.2 641,900 - ybdR b0608 0.48 +1.1 642,079 + ybdR b0608 0.62 +0.8 642,087 - ybdR b0608 0.63 -0.5 642,097 - ybdR b0608 0.63 -0.2 642,173 + ybdR b0608 0.70 +0.8 642,191 + ybdR b0608 0.71 +0.5 642,218 + ybdR b0608 0.73 +0.7 642,317 - ybdR b0608 0.81 +0.5 642,412 + ybdR b0608 0.89 +0.7 642,665 + +1.0 642,665 + +0.6 642,747 - -1.0 642,750 - -2.6 642,750 - +0.3 642,784 + +1.0 642,785 - +0.2 642,890 + rnk b0610 0.27 -0.5 642,922 + rnk b0610 0.35 +0.6 643,290 - -0.5 643,339 + +1.1 643,347 - +0.9 643,530 + rna b0611 0.14 +0.8
Or see this region's nucleotide sequence