Experiment: 1/10ON stiff
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mhpD and mhpF overlap by 4 nucleotides mhpF and mhpE overlap by 4 nucleotides mhpE and mhpT are separated by 577 nucleotides
b0350: mhpD - 2-keto-4-pentenoate hydratase (VIMSS), at 371,339 to 372,148
mhpD
b0351: mhpF - acetaldehyde dehydrogenase (NCBI), at 372,145 to 373,095
mhpF
b0352: mhpE - 4-hydroxy-2-ketovalerate aldolase (NCBI), at 373,092 to 374,105
mhpE
b0353: mhpT - putative transport protein (VIMSS), at 374,683 to 375,894
mhpT
Position (kb) 373
374
375 Strain fitness (log2 ratio) -2
-1
0
1
2 at 372.181 kb on - strand at 372.181 kb on - strand at 372.191 kb on - strand at 372.191 kb on - strand at 372.193 kb on + strand at 372.193 kb on + strand at 372.226 kb on + strand at 372.226 kb on + strand at 372.271 kb on + strand, within mhpF at 372.307 kb on + strand, within mhpF at 372.308 kb on + strand, within mhpF at 372.308 kb on + strand, within mhpF at 372.308 kb on + strand, within mhpF at 372.619 kb on - strand, within mhpF at 372.648 kb on + strand, within mhpF at 372.779 kb on - strand, within mhpF at 372.779 kb on - strand, within mhpF at 372.793 kb on + strand, within mhpF at 372.814 kb on + strand, within mhpF at 372.820 kb on + strand, within mhpF at 372.844 kb on - strand, within mhpF at 372.854 kb on - strand, within mhpF at 372.903 kb on + strand, within mhpF at 372.991 kb on + strand, within mhpF at 372.991 kb on + strand, within mhpF at 373.062 kb on + strand at 373.130 kb on + strand at 373.130 kb on + strand at 373.393 kb on + strand, within mhpE at 373.429 kb on + strand, within mhpE at 373.429 kb on + strand, within mhpE at 373.437 kb on - strand, within mhpE at 373.552 kb on - strand, within mhpE at 373.576 kb on + strand, within mhpE at 373.576 kb on + strand, within mhpE at 373.640 kb on + strand, within mhpE at 373.640 kb on + strand, within mhpE at 373.736 kb on - strand, within mhpE at 373.787 kb on - strand, within mhpE at 373.787 kb on - strand, within mhpE at 373.857 kb on - strand, within mhpE at 373.857 kb on - strand, within mhpE at 373.912 kb on - strand, within mhpE at 374.415 kb on + strand at 374.631 kb on - strand at 374.643 kb on - strand at 374.643 kb on - strand at 374.686 kb on - strand at 374.686 kb on - strand at 374.715 kb on - strand at 374.796 kb on + strand at 374.796 kb on + strand at 374.877 kb on - strand, within mhpT at 374.877 kb on - strand, within mhpT at 374.914 kb on + strand, within mhpT at 375.030 kb on + strand, within mhpT at 375.030 kb on + strand, within mhpT at 375.044 kb on + strand, within mhpT at 375.048 kb on - strand, within mhpT
Per-strain Table
Position Strand Gene LocusTag Fraction 1/10ON stiff remove 372,181 - -1.1 372,181 - +0.5 372,191 - +0.7 372,191 - -0.1 372,193 + +0.7 372,193 + +0.6 372,226 + +1.8 372,226 + -0.9 372,271 + mhpF b0351 0.13 +0.5 372,307 + mhpF b0351 0.17 +0.8 372,308 + mhpF b0351 0.17 +1.3 372,308 + mhpF b0351 0.17 +0.3 372,308 + mhpF b0351 0.17 -1.8 372,619 - mhpF b0351 0.50 +1.3 372,648 + mhpF b0351 0.53 -0.1 372,779 - mhpF b0351 0.67 +1.5 372,779 - mhpF b0351 0.67 -1.9 372,793 + mhpF b0351 0.68 +0.2 372,814 + mhpF b0351 0.70 +0.1 372,820 + mhpF b0351 0.71 -0.5 372,844 - mhpF b0351 0.74 +0.0 372,854 - mhpF b0351 0.75 +0.2 372,903 + mhpF b0351 0.80 +0.8 372,991 + mhpF b0351 0.89 +1.1 372,991 + mhpF b0351 0.89 -0.7 373,062 + +0.6 373,130 + +0.1 373,130 + +0.3 373,393 + mhpE b0352 0.30 +0.2 373,429 + mhpE b0352 0.33 +0.8 373,429 + mhpE b0352 0.33 -0.7 373,437 - mhpE b0352 0.34 +0.9 373,552 - mhpE b0352 0.45 +0.0 373,576 + mhpE b0352 0.48 +0.7 373,576 + mhpE b0352 0.48 -0.1 373,640 + mhpE b0352 0.54 +0.5 373,640 + mhpE b0352 0.54 -1.3 373,736 - mhpE b0352 0.64 -0.3 373,787 - mhpE b0352 0.69 +0.5 373,787 - mhpE b0352 0.69 +0.4 373,857 - mhpE b0352 0.75 +0.2 373,857 - mhpE b0352 0.75 -0.3 373,912 - mhpE b0352 0.81 -0.3 374,415 + +0.6 374,631 - -1.3 374,643 - +0.7 374,643 - +1.0 374,686 - +1.6 374,686 - +0.3 374,715 - +0.3 374,796 + +0.4 374,796 + +0.6 374,877 - mhpT b0353 0.16 +0.6 374,877 - mhpT b0353 0.16 -0.2 374,914 + mhpT b0353 0.19 +1.5 375,030 + mhpT b0353 0.29 +0.1 375,030 + mhpT b0353 0.29 +0.1 375,044 + mhpT b0353 0.30 +0.6 375,048 - mhpT b0353 0.30 +1.1
Or see this region's nucleotide sequence