Experiment: 1/10ON soft
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhfW and yhfX overlap by 1 nucleotides yhfX and yhfY are separated by 83 nucleotides yhfY and yhfZ are separated by 16 nucleotides
b3380: yhfW - predicted mutase (NCBI), at 3,506,609 to 3,507,835
yhfW
b3381: yhfX - predicted amino acid racemase (NCBI), at 3,507,835 to 3,508,998
yhfX
b3382: yhfY - orf, hypothetical protein (VIMSS), at 3,509,082 to 3,509,444
yhfY
b3383: yhfZ - hypothetical protein (NCBI), at 3,509,461 to 3,510,366
yhfZ
Position (kb)
3507
3508
3509 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3506.858 kb on + strand, within yhfW at 3506.885 kb on - strand, within yhfW at 3506.950 kb on + strand, within yhfW at 3506.976 kb on + strand, within yhfW at 3507.017 kb on - strand, within yhfW at 3507.017 kb on - strand, within yhfW at 3507.078 kb on + strand, within yhfW at 3507.078 kb on + strand, within yhfW at 3507.089 kb on - strand, within yhfW at 3507.089 kb on - strand, within yhfW at 3507.094 kb on + strand, within yhfW at 3507.108 kb on - strand, within yhfW at 3507.155 kb on + strand, within yhfW at 3507.155 kb on + strand, within yhfW at 3507.157 kb on + strand, within yhfW at 3507.169 kb on + strand, within yhfW at 3507.169 kb on + strand, within yhfW at 3507.189 kb on + strand, within yhfW at 3507.205 kb on + strand, within yhfW at 3507.213 kb on - strand, within yhfW at 3507.242 kb on + strand, within yhfW at 3507.242 kb on + strand, within yhfW at 3507.274 kb on - strand, within yhfW at 3507.286 kb on - strand, within yhfW at 3507.300 kb on + strand, within yhfW at 3507.300 kb on + strand, within yhfW at 3507.300 kb on + strand, within yhfW at 3507.308 kb on - strand, within yhfW at 3507.308 kb on - strand, within yhfW at 3507.310 kb on + strand, within yhfW at 3507.327 kb on - strand, within yhfW at 3507.343 kb on + strand, within yhfW at 3507.386 kb on - strand, within yhfW at 3507.459 kb on - strand, within yhfW at 3507.472 kb on - strand, within yhfW at 3507.472 kb on - strand, within yhfW at 3507.525 kb on - strand, within yhfW at 3507.525 kb on - strand, within yhfW at 3507.574 kb on + strand, within yhfW at 3507.574 kb on + strand, within yhfW at 3507.607 kb on + strand, within yhfW at 3507.607 kb on + strand, within yhfW at 3507.664 kb on - strand, within yhfW at 3507.664 kb on - strand, within yhfW at 3507.688 kb on + strand, within yhfW at 3507.694 kb on + strand, within yhfW at 3507.710 kb on - strand, within yhfW at 3507.727 kb on + strand at 3507.741 kb on - strand at 3507.743 kb on + strand at 3507.743 kb on + strand at 3507.746 kb on - strand at 3507.746 kb on - strand at 3507.767 kb on + strand at 3507.767 kb on + strand at 3507.767 kb on + strand at 3507.796 kb on + strand at 3507.870 kb on - strand at 3507.899 kb on + strand at 3507.918 kb on - strand at 3507.955 kb on - strand, within yhfX at 3507.955 kb on - strand, within yhfX at 3507.960 kb on + strand, within yhfX at 3507.960 kb on + strand, within yhfX at 3507.960 kb on + strand, within yhfX at 3507.968 kb on - strand, within yhfX at 3507.968 kb on - strand, within yhfX at 3507.997 kb on - strand, within yhfX at 3508.001 kb on + strand, within yhfX at 3508.001 kb on + strand, within yhfX at 3508.013 kb on + strand, within yhfX at 3508.013 kb on - strand, within yhfX at 3508.013 kb on - strand, within yhfX at 3508.018 kb on - strand, within yhfX at 3508.101 kb on - strand, within yhfX at 3508.110 kb on - strand, within yhfX at 3508.178 kb on + strand, within yhfX at 3508.185 kb on + strand, within yhfX at 3508.255 kb on + strand, within yhfX at 3508.263 kb on - strand, within yhfX at 3508.369 kb on - strand, within yhfX at 3508.486 kb on + strand, within yhfX at 3508.563 kb on + strand, within yhfX at 3508.567 kb on - strand, within yhfX at 3508.634 kb on + strand, within yhfX at 3508.634 kb on + strand, within yhfX at 3508.650 kb on - strand, within yhfX at 3508.652 kb on - strand, within yhfX at 3508.675 kb on - strand, within yhfX at 3508.675 kb on - strand, within yhfX at 3508.685 kb on + strand, within yhfX at 3508.823 kb on + strand, within yhfX at 3508.831 kb on - strand, within yhfX at 3508.833 kb on + strand, within yhfX at 3508.849 kb on - strand, within yhfX at 3508.849 kb on - strand, within yhfX at 3508.917 kb on + strand at 3508.950 kb on - strand at 3509.069 kb on + strand at 3509.069 kb on - strand at 3509.112 kb on - strand at 3509.215 kb on - strand, within yhfY at 3509.254 kb on + strand, within yhfY at 3509.254 kb on + strand, within yhfY at 3509.317 kb on - strand, within yhfY at 3509.349 kb on - strand, within yhfY at 3509.395 kb on + strand, within yhfY at 3509.395 kb on + strand, within yhfY at 3509.447 kb on - strand at 3509.502 kb on - strand at 3509.520 kb on + strand at 3509.524 kb on + strand at 3509.529 kb on - strand at 3509.529 kb on - strand at 3509.537 kb on - strand at 3509.539 kb on - strand at 3509.539 kb on - strand at 3509.547 kb on - strand at 3509.553 kb on - strand, within yhfZ at 3509.572 kb on + strand, within yhfZ at 3509.572 kb on + strand, within yhfZ at 3509.576 kb on - strand, within yhfZ at 3509.639 kb on - strand, within yhfZ at 3509.723 kb on + strand, within yhfZ at 3509.803 kb on + strand, within yhfZ at 3509.803 kb on + strand, within yhfZ at 3509.875 kb on - strand, within yhfZ at 3509.888 kb on - strand, within yhfZ at 3509.888 kb on - strand, within yhfZ at 3509.926 kb on - strand, within yhfZ at 3509.966 kb on + strand, within yhfZ at 3509.966 kb on + strand, within yhfZ
Per-strain Table
Position Strand Gene LocusTag Fraction 1/10ON soft remove 3,506,858 + yhfW b3380 0.20 -1.8 3,506,885 - yhfW b3380 0.22 -0.1 3,506,950 + yhfW b3380 0.28 +0.3 3,506,976 + yhfW b3380 0.30 +0.2 3,507,017 - yhfW b3380 0.33 +0.3 3,507,017 - yhfW b3380 0.33 -0.2 3,507,078 + yhfW b3380 0.38 +0.8 3,507,078 + yhfW b3380 0.38 +0.5 3,507,089 - yhfW b3380 0.39 -2.6 3,507,089 - yhfW b3380 0.39 -0.6 3,507,094 + yhfW b3380 0.40 +0.3 3,507,108 - yhfW b3380 0.41 +0.5 3,507,155 + yhfW b3380 0.44 +0.6 3,507,155 + yhfW b3380 0.44 -1.4 3,507,157 + yhfW b3380 0.45 +0.5 3,507,169 + yhfW b3380 0.46 -0.0 3,507,169 + yhfW b3380 0.46 +0.3 3,507,189 + yhfW b3380 0.47 +1.1 3,507,205 + yhfW b3380 0.49 -0.8 3,507,213 - yhfW b3380 0.49 -0.2 3,507,242 + yhfW b3380 0.52 -0.0 3,507,242 + yhfW b3380 0.52 -0.5 3,507,274 - yhfW b3380 0.54 -0.9 3,507,286 - yhfW b3380 0.55 +0.4 3,507,300 + yhfW b3380 0.56 -0.1 3,507,300 + yhfW b3380 0.56 -0.3 3,507,300 + yhfW b3380 0.56 -0.4 3,507,308 - yhfW b3380 0.57 +0.1 3,507,308 - yhfW b3380 0.57 -0.4 3,507,310 + yhfW b3380 0.57 +0.4 3,507,327 - yhfW b3380 0.59 -0.3 3,507,343 + yhfW b3380 0.60 +0.2 3,507,386 - yhfW b3380 0.63 +0.7 3,507,459 - yhfW b3380 0.69 +0.5 3,507,472 - yhfW b3380 0.70 -1.0 3,507,472 - yhfW b3380 0.70 -0.5 3,507,525 - yhfW b3380 0.75 -0.2 3,507,525 - yhfW b3380 0.75 +1.0 3,507,574 + yhfW b3380 0.79 -0.8 3,507,574 + yhfW b3380 0.79 +0.1 3,507,607 + yhfW b3380 0.81 +0.3 3,507,607 + yhfW b3380 0.81 -0.7 3,507,664 - yhfW b3380 0.86 +0.2 3,507,664 - yhfW b3380 0.86 +0.2 3,507,688 + yhfW b3380 0.88 +0.7 3,507,694 + yhfW b3380 0.88 +0.1 3,507,710 - yhfW b3380 0.90 +0.1 3,507,727 + +0.5 3,507,741 - -0.1 3,507,743 + +0.3 3,507,743 + -0.2 3,507,746 - -0.2 3,507,746 - +1.5 3,507,767 + -0.1 3,507,767 + +0.6 3,507,767 + -1.2 3,507,796 + +0.4 3,507,870 - -0.3 3,507,899 + -0.2 3,507,918 - -0.5 3,507,955 - yhfX b3381 0.10 -0.2 3,507,955 - yhfX b3381 0.10 +0.2 3,507,960 + yhfX b3381 0.11 -0.4 3,507,960 + yhfX b3381 0.11 +0.6 3,507,960 + yhfX b3381 0.11 -0.8 3,507,968 - yhfX b3381 0.11 +0.5 3,507,968 - yhfX b3381 0.11 +0.0 3,507,997 - yhfX b3381 0.14 +0.9 3,508,001 + yhfX b3381 0.14 -0.2 3,508,001 + yhfX b3381 0.14 +0.8 3,508,013 + yhfX b3381 0.15 -0.1 3,508,013 - yhfX b3381 0.15 +0.2 3,508,013 - yhfX b3381 0.15 +0.0 3,508,018 - yhfX b3381 0.16 +0.5 3,508,101 - yhfX b3381 0.23 +0.0 3,508,110 - yhfX b3381 0.24 +0.3 3,508,178 + yhfX b3381 0.29 -0.5 3,508,185 + yhfX b3381 0.30 +0.4 3,508,255 + yhfX b3381 0.36 +0.1 3,508,263 - yhfX b3381 0.37 -1.1 3,508,369 - yhfX b3381 0.46 +0.6 3,508,486 + yhfX b3381 0.56 +0.5 3,508,563 + yhfX b3381 0.63 -0.4 3,508,567 - yhfX b3381 0.63 -0.4 3,508,634 + yhfX b3381 0.69 +0.1 3,508,634 + yhfX b3381 0.69 -0.9 3,508,650 - yhfX b3381 0.70 +0.1 3,508,652 - yhfX b3381 0.70 -0.1 3,508,675 - yhfX b3381 0.72 +1.9 3,508,675 - yhfX b3381 0.72 +0.0 3,508,685 + yhfX b3381 0.73 +0.1 3,508,823 + yhfX b3381 0.85 +0.2 3,508,831 - yhfX b3381 0.86 -0.9 3,508,833 + yhfX b3381 0.86 -0.8 3,508,849 - yhfX b3381 0.87 +1.3 3,508,849 - yhfX b3381 0.87 -0.7 3,508,917 + +0.8 3,508,950 - +0.0 3,509,069 + -1.8 3,509,069 - +0.6 3,509,112 - +0.6 3,509,215 - yhfY b3382 0.37 +1.1 3,509,254 + yhfY b3382 0.47 -0.5 3,509,254 + yhfY b3382 0.47 -0.1 3,509,317 - yhfY b3382 0.65 -0.2 3,509,349 - yhfY b3382 0.74 -1.0 3,509,395 + yhfY b3382 0.86 +0.3 3,509,395 + yhfY b3382 0.86 -0.2 3,509,447 - +0.2 3,509,502 - +0.1 3,509,520 + +0.9 3,509,524 + -0.6 3,509,529 - -0.2 3,509,529 - -0.2 3,509,537 - +0.2 3,509,539 - +0.5 3,509,539 - +1.9 3,509,547 - +1.0 3,509,553 - yhfZ b3383 0.10 -0.4 3,509,572 + yhfZ b3383 0.12 +1.3 3,509,572 + yhfZ b3383 0.12 -0.1 3,509,576 - yhfZ b3383 0.13 -0.1 3,509,639 - yhfZ b3383 0.20 +0.3 3,509,723 + yhfZ b3383 0.29 +1.2 3,509,803 + yhfZ b3383 0.38 -0.7 3,509,803 + yhfZ b3383 0.38 -0.2 3,509,875 - yhfZ b3383 0.46 -1.2 3,509,888 - yhfZ b3383 0.47 -1.0 3,509,888 - yhfZ b3383 0.47 -0.8 3,509,926 - yhfZ b3383 0.51 +0.4 3,509,966 + yhfZ b3383 0.56 +1.8 3,509,966 + yhfZ b3383 0.56 -0.2
Or see this region's nucleotide sequence