Strain Fitness in Escherichia coli BW25113 around b4319

Experiment: 1/2ON stiff

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfimD and fimF are separated by 9 nucleotidesfimF and fimG are separated by 12 nucleotidesfimG and fimH are separated by 19 nucleotides b4317: fimD - outer membrane usher protein, type 1 fimbrial synthesis (NCBI), at 4,543,119 to 4,545,755 fimD b4318: fimF - minor component of type 1 fimbriae (NCBI), at 4,545,765 to 4,546,295 fimF b4319: fimG - minor component of type 1 fimbriae (NCBI), at 4,546,308 to 4,546,811 fimG b4320: fimH - minor component of type 1 fimbriae (NCBI), at 4,546,831 to 4,547,733 fimH Position (kb) 4546 4547Strain fitness (log2 ratio) -1 0 1 2 3at 4545.345 kb on + strand, within fimDat 4545.354 kb on - strand, within fimDat 4545.354 kb on - strand, within fimDat 4545.367 kb on - strand, within fimDat 4545.391 kb on - strand, within fimDat 4545.393 kb on + strand, within fimDat 4545.401 kb on - strand, within fimDat 4545.403 kb on - strand, within fimDat 4545.468 kb on - strand, within fimDat 4545.468 kb on - strand, within fimDat 4545.485 kb on + strand, within fimDat 4545.519 kb on + strandat 4545.555 kb on - strandat 4545.555 kb on - strandat 4545.565 kb on + strandat 4545.612 kb on - strandat 4545.724 kb on + strandat 4545.734 kb on + strandat 4545.759 kb on + strandat 4545.759 kb on + strandat 4545.874 kb on + strandat 4546.062 kb on - strand, within fimFat 4546.062 kb on - strandat 4546.251 kb on - strandat 4546.251 kb on - strandat 4546.259 kb on - strandat 4546.289 kb on - strandat 4546.292 kb on + strandat 4546.365 kb on - strand, within fimGat 4546.373 kb on - strand, within fimGat 4546.373 kb on - strand, within fimGat 4546.373 kb on - strand, within fimGat 4546.411 kb on + strand, within fimGat 4546.419 kb on - strand, within fimGat 4546.429 kb on - strandat 4546.511 kb on - strand, within fimGat 4546.511 kb on - strand, within fimGat 4546.643 kb on - strand, within fimGat 4546.647 kb on + strand, within fimGat 4546.650 kb on + strand, within fimGat 4546.657 kb on - strand, within fimGat 4546.669 kb on - strand, within fimGat 4546.669 kb on - strand, within fimGat 4546.684 kb on - strand, within fimGat 4546.794 kb on + strandat 4546.828 kb on + strandat 4546.859 kb on - strandat 4546.871 kb on - strandat 4546.874 kb on - strandat 4546.882 kb on - strandat 4546.882 kb on - strandat 4547.047 kb on + strand, within fimHat 4547.047 kb on + strand, within fimHat 4547.055 kb on + strand, within fimHat 4547.055 kb on + strand, within fimHat 4547.057 kb on + strand, within fimHat 4547.057 kb on + strand, within fimHat 4547.057 kb on + strand, within fimHat 4547.059 kb on + strand, within fimHat 4547.065 kb on - strand, within fimHat 4547.066 kb on - strand, within fimHat 4547.067 kb on + strand, within fimHat 4547.080 kb on + strand, within fimHat 4547.080 kb on + strand, within fimHat 4547.080 kb on + strand, within fimHat 4547.080 kb on + strand, within fimHat 4547.145 kb on + strand, within fimHat 4547.224 kb on + strand, within fimHat 4547.232 kb on - strand, within fimHat 4547.232 kb on - strand, within fimHat 4547.326 kb on + strand, within fimHat 4547.361 kb on + strand, within fimHat 4547.361 kb on + strand, within fimHat 4547.361 kb on + strand, within fimHat 4547.361 kb on + strand, within fimHat 4547.363 kb on + strand, within fimHat 4547.367 kb on - strand, within fimHat 4547.367 kb on - strand, within fimHat 4547.369 kb on - strand, within fimHat 4547.369 kb on - strand, within fimHat 4547.369 kb on - strand, within fimHat 4547.387 kb on + strand, within fimHat 4547.387 kb on + strand, within fimHat 4547.407 kb on + strand, within fimHat 4547.407 kb on + strand, within fimHat 4547.417 kb on + strand, within fimHat 4547.417 kb on + strand, within fimHat 4547.417 kb on + strand, within fimHat 4547.425 kb on - strand, within fimHat 4547.481 kb on - strand, within fimHat 4547.483 kb on - strand, within fimHat 4547.490 kb on - strand, within fimHat 4547.490 kb on - strand, within fimHat 4547.542 kb on + strand, within fimHat 4547.542 kb on + strand, within fimHat 4547.558 kb on - strand, within fimHat 4547.565 kb on + strand, within fimHat 4547.647 kb on - strandat 4547.647 kb on - strandat 4547.666 kb on + strandat 4547.747 kb on - strandat 4547.763 kb on + strandat 4547.766 kb on + strandat 4547.766 kb on - strandat 4547.768 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1/2ON stiff
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4,545,345 + fimD b4317 0.84 -0.2
4,545,354 - fimD b4317 0.85 -0.2
4,545,354 - fimD b4317 0.85 +0.1
4,545,367 - fimD b4317 0.85 +0.1
4,545,391 - fimD b4317 0.86 +0.4
4,545,393 + fimD b4317 0.86 +0.5
4,545,401 - fimD b4317 0.87 +0.3
4,545,403 - fimD b4317 0.87 +0.7
4,545,468 - fimD b4317 0.89 +0.5
4,545,468 - fimD b4317 0.89 -0.0
4,545,485 + fimD b4317 0.90 -0.2
4,545,519 + +0.5
4,545,555 - -0.3
4,545,555 - +0.4
4,545,565 + -0.2
4,545,612 - +0.4
4,545,724 + +0.4
4,545,734 + +2.3
4,545,759 + -0.4
4,545,759 + -0.4
4,545,874 + +2.8
4,546,062 - fimF b4318 0.56 +0.3
4,546,062 - -1.4
4,546,251 - -0.1
4,546,251 - -0.4
4,546,259 - -0.2
4,546,289 - +0.1
4,546,292 + +0.5
4,546,365 - fimG b4319 0.11 +0.1
4,546,373 - fimG b4319 0.13 -0.2
4,546,373 - fimG b4319 0.13 +0.3
4,546,373 - fimG b4319 0.13 +0.1
4,546,411 + fimG b4319 0.20 +0.1
4,546,419 - fimG b4319 0.22 +0.0
4,546,429 - +1.2
4,546,511 - fimG b4319 0.40 -0.3
4,546,511 - fimG b4319 0.40 +0.2
4,546,643 - fimG b4319 0.66 +0.2
4,546,647 + fimG b4319 0.67 +0.1
4,546,650 + fimG b4319 0.68 +0.1
4,546,657 - fimG b4319 0.69 +0.9
4,546,669 - fimG b4319 0.72 +0.1
4,546,669 - fimG b4319 0.72 +0.1
4,546,684 - fimG b4319 0.75 -0.2
4,546,794 + +0.1
4,546,828 + +0.2
4,546,859 - -1.4
4,546,871 - -0.3
4,546,874 - +0.8
4,546,882 - -0.1
4,546,882 - +0.1
4,547,047 + fimH b4320 0.24 +0.6
4,547,047 + fimH b4320 0.24 +0.6
4,547,055 + fimH b4320 0.25 +0.6
4,547,055 + fimH b4320 0.25 +1.0
4,547,057 + fimH b4320 0.25 -0.1
4,547,057 + fimH b4320 0.25 +0.3
4,547,057 + fimH b4320 0.25 +0.1
4,547,059 + fimH b4320 0.25 +1.5
4,547,065 - fimH b4320 0.26 +0.0
4,547,066 - fimH b4320 0.26 +0.6
4,547,067 + fimH b4320 0.26 -0.9
4,547,080 + fimH b4320 0.28 +1.2
4,547,080 + fimH b4320 0.28 +0.5
4,547,080 + fimH b4320 0.28 -0.7
4,547,080 + fimH b4320 0.28 +0.2
4,547,145 + fimH b4320 0.35 +0.5
4,547,224 + fimH b4320 0.44 +0.5
4,547,232 - fimH b4320 0.44 +0.1
4,547,232 - fimH b4320 0.44 -0.1
4,547,326 + fimH b4320 0.55 +0.3
4,547,361 + fimH b4320 0.59 +0.6
4,547,361 + fimH b4320 0.59 +1.0
4,547,361 + fimH b4320 0.59 +0.8
4,547,361 + fimH b4320 0.59 +0.6
4,547,363 + fimH b4320 0.59 -0.2
4,547,367 - fimH b4320 0.59 +0.2
4,547,367 - fimH b4320 0.59 +0.9
4,547,369 - fimH b4320 0.60 +2.0
4,547,369 - fimH b4320 0.60 +0.5
4,547,369 - fimH b4320 0.60 +1.6
4,547,387 + fimH b4320 0.62 +0.2
4,547,387 + fimH b4320 0.62 -0.4
4,547,407 + fimH b4320 0.64 +0.7
4,547,407 + fimH b4320 0.64 +1.1
4,547,417 + fimH b4320 0.65 +0.3
4,547,417 + fimH b4320 0.65 -0.0
4,547,417 + fimH b4320 0.65 -0.5
4,547,425 - fimH b4320 0.66 +0.3
4,547,481 - fimH b4320 0.72 +1.8
4,547,483 - fimH b4320 0.72 +0.2
4,547,490 - fimH b4320 0.73 +0.1
4,547,490 - fimH b4320 0.73 -0.8
4,547,542 + fimH b4320 0.79 -0.1
4,547,542 + fimH b4320 0.79 +0.2
4,547,558 - fimH b4320 0.81 -0.2
4,547,565 + fimH b4320 0.81 -0.2
4,547,647 - +0.6
4,547,647 - -0.3
4,547,666 + -0.0
4,547,747 - -0.9
4,547,763 + +0.3
4,547,766 + +0.6
4,547,766 - -0.4
4,547,768 + -0.1

Or see this region's nucleotide sequence