Experiment: 1/2ON stiff
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygbL and ygbM are separated by 4 nucleotides ygbM and ygbN are separated by 88 nucleotides ygbN and rpoS are separated by 93 nucleotides
b2738: ygbL - hypothetical protein (NCBI), at 2,861,615 to 2,862,253
ygbL
b2739: ygbM - hypothetical protein (NCBI), at 2,862,258 to 2,863,034
ygbM
b2740: ygbN - predicted transporter (NCBI), at 2,863,123 to 2,864,487
ygbN
b2741: rpoS - RNA polymerase sigma factor (NCBI), at 2,864,581 to 2,865,573
rpoS
Position (kb)
2863
2864
2865 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2862.384 kb on - strand, within ygbM at 2862.439 kb on - strand, within ygbM at 2862.439 kb on - strand, within ygbM at 2862.479 kb on - strand, within ygbM at 2862.511 kb on - strand, within ygbM at 2862.531 kb on + strand, within ygbM at 2862.569 kb on - strand, within ygbM at 2862.569 kb on - strand, within ygbM at 2862.598 kb on + strand, within ygbM at 2862.608 kb on + strand, within ygbM at 2862.660 kb on - strand, within ygbM at 2862.695 kb on - strand, within ygbM at 2862.708 kb on - strand, within ygbM at 2862.741 kb on - strand, within ygbM at 2862.743 kb on + strand, within ygbM at 2862.743 kb on + strand, within ygbM at 2862.753 kb on - strand, within ygbM at 2862.756 kb on - strand, within ygbM at 2862.767 kb on + strand, within ygbM at 2862.775 kb on - strand, within ygbM at 2862.775 kb on - strand, within ygbM at 2862.898 kb on - strand, within ygbM at 2862.903 kb on - strand, within ygbM at 2862.903 kb on - strand, within ygbM at 2862.936 kb on - strand, within ygbM at 2862.947 kb on + strand, within ygbM at 2862.947 kb on + strand, within ygbM at 2862.967 kb on - strand at 2862.997 kb on + strand at 2863.069 kb on - strand at 2863.084 kb on - strand at 2863.114 kb on + strand at 2863.140 kb on + strand at 2863.261 kb on - strand, within ygbN at 2863.261 kb on - strand, within ygbN at 2863.338 kb on - strand, within ygbN at 2863.342 kb on + strand, within ygbN at 2863.342 kb on + strand, within ygbN at 2863.342 kb on + strand, within ygbN at 2863.370 kb on + strand, within ygbN at 2863.370 kb on + strand, within ygbN at 2863.435 kb on - strand, within ygbN at 2863.571 kb on - strand, within ygbN at 2863.627 kb on + strand, within ygbN at 2863.627 kb on + strand, within ygbN at 2863.627 kb on + strand, within ygbN at 2863.627 kb on + strand, within ygbN at 2863.693 kb on - strand, within ygbN at 2863.751 kb on + strand, within ygbN at 2863.782 kb on - strand, within ygbN at 2863.832 kb on + strand, within ygbN at 2863.861 kb on - strand, within ygbN at 2863.861 kb on - strand, within ygbN at 2863.933 kb on + strand, within ygbN at 2863.961 kb on - strand, within ygbN at 2863.961 kb on - strand, within ygbN at 2864.020 kb on + strand, within ygbN at 2864.020 kb on + strand, within ygbN at 2864.026 kb on - strand, within ygbN at 2864.036 kb on + strand, within ygbN at 2864.038 kb on + strand, within ygbN at 2864.038 kb on + strand, within ygbN at 2864.101 kb on - strand, within ygbN at 2864.148 kb on - strand, within ygbN at 2864.150 kb on + strand, within ygbN at 2864.231 kb on - strand, within ygbN at 2864.231 kb on - strand, within ygbN at 2864.395 kb on + strand at 2864.422 kb on + strand at 2864.463 kb on - strand at 2864.590 kb on + strand at 2864.608 kb on - strand at 2864.608 kb on - strand at 2864.641 kb on + strand at 2864.656 kb on - strand at 2864.698 kb on + strand, within rpoS at 2864.840 kb on - strand at 2864.866 kb on + strand, within rpoS at 2864.925 kb on + strand, within rpoS at 2864.925 kb on + strand, within rpoS at 2865.021 kb on + strand, within rpoS at 2865.037 kb on + strand, within rpoS at 2865.044 kb on + strand, within rpoS at 2865.047 kb on - strand, within rpoS at 2865.085 kb on - strand, within rpoS at 2865.116 kb on - strand, within rpoS at 2865.175 kb on + strand, within rpoS at 2865.190 kb on + strand, within rpoS at 2865.195 kb on + strand, within rpoS at 2865.198 kb on - strand, within rpoS at 2865.376 kb on - strand, within rpoS at 2865.389 kb on + strand, within rpoS at 2865.415 kb on + strand, within rpoS at 2865.447 kb on - strand, within rpoS
Per-strain Table
Position Strand Gene LocusTag Fraction 1/2ON stiff remove 2,862,384 - ygbM b2739 0.16 +0.4 2,862,439 - ygbM b2739 0.23 -0.3 2,862,439 - ygbM b2739 0.23 +0.9 2,862,479 - ygbM b2739 0.28 -0.0 2,862,511 - ygbM b2739 0.33 +0.3 2,862,531 + ygbM b2739 0.35 +0.1 2,862,569 - ygbM b2739 0.40 -1.0 2,862,569 - ygbM b2739 0.40 +1.4 2,862,598 + ygbM b2739 0.44 -1.8 2,862,608 + ygbM b2739 0.45 +1.0 2,862,660 - ygbM b2739 0.52 +0.9 2,862,695 - ygbM b2739 0.56 +1.6 2,862,708 - ygbM b2739 0.58 +0.2 2,862,741 - ygbM b2739 0.62 +0.4 2,862,743 + ygbM b2739 0.62 -0.7 2,862,743 + ygbM b2739 0.62 -0.8 2,862,753 - ygbM b2739 0.64 +2.3 2,862,756 - ygbM b2739 0.64 -0.7 2,862,767 + ygbM b2739 0.66 +0.4 2,862,775 - ygbM b2739 0.67 -0.8 2,862,775 - ygbM b2739 0.67 +0.8 2,862,898 - ygbM b2739 0.82 -2.3 2,862,903 - ygbM b2739 0.83 +0.8 2,862,903 - ygbM b2739 0.83 -0.7 2,862,936 - ygbM b2739 0.87 +0.2 2,862,947 + ygbM b2739 0.89 +1.2 2,862,947 + ygbM b2739 0.89 +0.4 2,862,967 - -1.7 2,862,997 + -0.3 2,863,069 - -0.1 2,863,084 - +0.2 2,863,114 + +0.4 2,863,140 + -0.5 2,863,261 - ygbN b2740 0.10 -0.4 2,863,261 - ygbN b2740 0.10 +0.7 2,863,338 - ygbN b2740 0.16 +0.7 2,863,342 + ygbN b2740 0.16 +0.2 2,863,342 + ygbN b2740 0.16 -0.3 2,863,342 + ygbN b2740 0.16 +0.0 2,863,370 + ygbN b2740 0.18 +0.9 2,863,370 + ygbN b2740 0.18 +0.3 2,863,435 - ygbN b2740 0.23 +0.7 2,863,571 - ygbN b2740 0.33 +0.9 2,863,627 + ygbN b2740 0.37 +0.5 2,863,627 + ygbN b2740 0.37 +0.2 2,863,627 + ygbN b2740 0.37 +0.1 2,863,627 + ygbN b2740 0.37 +0.1 2,863,693 - ygbN b2740 0.42 +0.3 2,863,751 + ygbN b2740 0.46 -0.3 2,863,782 - ygbN b2740 0.48 +1.0 2,863,832 + ygbN b2740 0.52 -0.7 2,863,861 - ygbN b2740 0.54 -0.3 2,863,861 - ygbN b2740 0.54 +0.3 2,863,933 + ygbN b2740 0.59 +0.9 2,863,961 - ygbN b2740 0.61 +0.4 2,863,961 - ygbN b2740 0.61 -0.5 2,864,020 + ygbN b2740 0.66 +0.2 2,864,020 + ygbN b2740 0.66 +0.0 2,864,026 - ygbN b2740 0.66 +0.0 2,864,036 + ygbN b2740 0.67 +0.1 2,864,038 + ygbN b2740 0.67 +0.3 2,864,038 + ygbN b2740 0.67 +0.3 2,864,101 - ygbN b2740 0.72 -1.8 2,864,148 - ygbN b2740 0.75 +0.5 2,864,150 + ygbN b2740 0.75 +0.5 2,864,231 - ygbN b2740 0.81 +0.7 2,864,231 - ygbN b2740 0.81 +0.2 2,864,395 + -0.0 2,864,422 + +0.3 2,864,463 - +0.1 2,864,590 + -2.3 2,864,608 - -0.5 2,864,608 - -1.0 2,864,641 + -0.0 2,864,656 - -1.8 2,864,698 + rpoS b2741 0.12 -0.9 2,864,840 - +1.3 2,864,866 + rpoS b2741 0.29 -0.7 2,864,925 + rpoS b2741 0.35 +0.6 2,864,925 + rpoS b2741 0.35 -1.0 2,865,021 + rpoS b2741 0.44 -0.4 2,865,037 + rpoS b2741 0.46 -0.3 2,865,044 + rpoS b2741 0.47 -0.6 2,865,047 - rpoS b2741 0.47 -0.7 2,865,085 - rpoS b2741 0.51 -1.6 2,865,116 - rpoS b2741 0.54 -0.1 2,865,175 + rpoS b2741 0.60 +0.3 2,865,190 + rpoS b2741 0.61 -0.7 2,865,195 + rpoS b2741 0.62 -0.3 2,865,198 - rpoS b2741 0.62 -2.3 2,865,376 - rpoS b2741 0.80 +1.1 2,865,389 + rpoS b2741 0.81 +0.1 2,865,415 + rpoS b2741 0.84 +0.3 2,865,447 - rpoS b2741 0.87 +0.3
Or see this region's nucleotide sequence