Strain Fitness in Escherichia coli BW25113 around b0947

Experiment: 1/2ON stiff

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	1/2ON stiff
1,004,717	+	pyrD	b0945	0.72	-1.0
1,004,720	-	pyrD	b0945	0.72	-1.1
1,004,722	+	pyrD	b0945	0.72	+0.7
1,004,727	+	pyrD	b0945	0.73	-0.5
1,004,727	+	pyrD	b0945	0.73	+0.4
1,004,733	-	pyrD	b0945	0.73	-0.1
1,004,733	-	pyrD	b0945	0.73	-0.1
1,004,736	-	pyrD	b0945	0.74	-0.1
1,004,780	-	pyrD	b0945	0.78	-0.1
1,004,783	+	pyrD	b0945	0.78	+0.2
1,004,785	+	pyrD	b0945	0.79	-1.2
1,004,791	-	pyrD	b0945	0.79	-1.6
1,004,791	-	pyrD	b0945	0.79	-0.6
1,004,801	-	pyrD	b0945	0.80	-2.1
1,004,871	+	pyrD	b0945	0.87	+0.2
1,004,871	+	pyrD	b0945	0.87	-0.1
1,004,871	+	pyrD	b0945	0.87	-0.2
1,004,888	-	pyrD	b0945	0.89	-0.1
1,004,938	+				-0.6
1,004,938	+				-0.9
1,005,020	-				-1.6
1,005,067	-				-0.3
1,005,108	-				+0.2
1,005,124	+				-0.5
1,005,137	-				-0.4
1,005,150	+				-1.1
1,005,152	+				+0.1
1,005,160	+				+1.5
1,005,166	-				-0.1
1,005,166	-				+0.2
1,005,168	-				+0.4
1,005,168	-				+0.4
1,005,170	-				+0.1
1,005,215	-				+0.1
1,005,227	+				+0.5
1,005,228	-				+0.9
1,005,228	-				+1.5
1,005,261	-	ycbW	b0946	0.16	+0.7
1,005,331	-	ycbW	b0946	0.29	+1.5
1,005,405	+	ycbW	b0946	0.42	+2.5
1,005,408	+	ycbW	b0946	0.43	-1.1
1,005,408	+	ycbW	b0946	0.43	-0.1
1,005,413	-	ycbW	b0946	0.44	+0.5
1,005,413	-	ycbW	b0946	0.44	-1.2
1,005,414	-	ycbW	b0946	0.44	+1.3
1,005,414	-	ycbW	b0946	0.44	-1.1
1,005,425	-	ycbW	b0946	0.46	+0.5
1,005,515	+	ycbW	b0946	0.63	+1.5
1,005,522	+	ycbW	b0946	0.64	+0.1
1,005,628	+	ycbW	b0946	0.83	-0.6
1,005,712	+				-0.3
1,005,720	-				-0.2
1,005,973	-	ycbX	b0947	0.23	+0.8
1,005,981	-	ycbX	b0947	0.24	+0.2
1,006,113	+	ycbX	b0947	0.36	+1.1
1,006,113	+	ycbX	b0947	0.36	+0.4
1,006,191	-	ycbX	b0947	0.43	+1.7
1,006,274	-	ycbX	b0947	0.50	+0.8
1,006,280	-	ycbX	b0947	0.51	-0.3
1,006,280	-	ycbX	b0947	0.51	+1.1
1,006,309	-	ycbX	b0947	0.54	+0.3
1,006,402	+	ycbX	b0947	0.62	+0.6
1,006,610	-	ycbX	b0947	0.81	+0.8
1,006,648	+	ycbX	b0947	0.84	+0.3
1,006,731	+				+1.2
1,006,731	+				+1.4
1,006,820	+				+0.4
1,006,962	-				-0.3
1,006,980	-				-0.9
1,006,980	-				-0.9
1,006,983	-				-1.2
1,007,045	-				-0.4
1,007,084	-				-0.4
1,007,092	+				-0.4
1,007,234	-				-0.1
1,007,282	+	ycbY	b0948	0.10	+0.0
1,007,387	-	ycbY	b0948	0.15	-1.5
1,007,387	-	ycbY	b0948	0.15	-1.1
1,007,481	+	ycbY	b0948	0.20	+1.1
1,007,481	+	ycbY	b0948	0.20	-0.4