Experiment: 1/2ON stiff
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ahpC and ahpF are separated by 244 nucleotides ahpF and uspG are separated by 120 nucleotides uspG and ybdR are separated by 220 nucleotides
b0605: ahpC - alkyl hydroperoxide reductase, C22 subunit (NCBI), at 638,168 to 638,731
ahpC
b0606: ahpF - alkyl hydroperoxide reductase, F52a subunit; detoxification of hydroperoxides (VIMSS), at 638,976 to 640,541
ahpF
b0607: uspG - universal stress protein UP12 (NCBI), at 640,662 to 641,090
uspG
b0608: ybdR - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI), at 641,311 to 642,549
ybdR
Position (kb) 638
639
640
641 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 638.144 kb on + strand at 638.144 kb on + strand at 638.315 kb on + strand, within ahpC at 638.376 kb on - strand, within ahpC at 638.378 kb on + strand, within ahpC at 638.489 kb on - strand, within ahpC at 638.513 kb on + strand, within ahpC at 638.513 kb on + strand, within ahpC at 638.557 kb on + strand, within ahpC at 638.557 kb on + strand, within ahpC at 638.557 kb on + strand, within ahpC at 638.625 kb on + strand, within ahpC at 638.692 kb on + strand at 638.923 kb on - strand at 638.923 kb on - strand at 638.937 kb on - strand at 639.002 kb on + strand at 639.227 kb on - strand, within ahpF at 639.227 kb on - strand, within ahpF at 639.268 kb on - strand, within ahpF at 639.428 kb on - strand, within ahpF at 639.448 kb on - strand, within ahpF at 639.639 kb on - strand, within ahpF at 639.681 kb on + strand, within ahpF at 639.943 kb on + strand, within ahpF at 639.943 kb on + strand, within ahpF at 639.978 kb on + strand, within ahpF at 640.001 kb on + strand, within ahpF at 640.169 kb on - strand, within ahpF at 640.169 kb on - strand, within ahpF at 640.173 kb on - strand, within ahpF at 640.314 kb on + strand, within ahpF at 640.364 kb on + strand, within ahpF at 640.391 kb on - strand at 640.391 kb on - strand at 640.447 kb on - strand at 640.486 kb on + strand at 640.486 kb on + strand at 640.605 kb on + strand at 640.605 kb on + strand at 640.637 kb on - strand at 640.637 kb on - strand at 640.642 kb on - strand at 640.647 kb on - strand at 640.647 kb on - strand at 640.670 kb on + strand at 640.670 kb on + strand at 640.782 kb on - strand, within uspG at 640.787 kb on - strand, within uspG at 640.787 kb on - strand, within uspG at 640.808 kb on - strand, within uspG at 640.813 kb on - strand, within uspG at 640.824 kb on - strand, within uspG at 640.863 kb on - strand, within uspG at 640.863 kb on - strand, within uspG at 640.887 kb on + strand, within uspG at 640.889 kb on + strand, within uspG at 640.889 kb on + strand, within uspG at 640.949 kb on + strand, within uspG at 640.949 kb on + strand, within uspG at 640.958 kb on + strand, within uspG at 640.976 kb on + strand, within uspG at 640.996 kb on - strand, within uspG at 641.001 kb on + strand, within uspG at 641.001 kb on + strand, within uspG at 641.075 kb on + strand at 641.080 kb on + strand at 641.080 kb on + strand at 641.080 kb on + strand at 641.080 kb on + strand at 641.088 kb on - strand at 641.088 kb on - strand at 641.139 kb on - strand at 641.221 kb on - strand at 641.221 kb on - strand at 641.223 kb on + strand at 641.266 kb on - strand at 641.266 kb on - strand at 641.286 kb on + strand at 641.286 kb on + strand at 641.342 kb on + strand at 641.342 kb on + strand at 641.372 kb on + strand at 641.372 kb on + strand at 641.434 kb on - strand at 641.434 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1/2ON stiff remove 638,144 + -0.3 638,144 + +0.3 638,315 + ahpC b0605 0.26 -0.7 638,376 - ahpC b0605 0.37 -2.2 638,378 + ahpC b0605 0.37 +0.3 638,489 - ahpC b0605 0.57 -3.2 638,513 + ahpC b0605 0.61 -0.9 638,513 + ahpC b0605 0.61 -1.8 638,557 + ahpC b0605 0.69 -0.8 638,557 + ahpC b0605 0.69 +0.4 638,557 + ahpC b0605 0.69 -0.3 638,625 + ahpC b0605 0.81 +2.4 638,692 + -1.9 638,923 - -1.1 638,923 - -0.3 638,937 - -2.2 639,002 + -0.9 639,227 - ahpF b0606 0.16 -3.5 639,227 - ahpF b0606 0.16 -1.1 639,268 - ahpF b0606 0.19 +1.4 639,428 - ahpF b0606 0.29 -0.7 639,448 - ahpF b0606 0.30 -1.4 639,639 - ahpF b0606 0.42 -0.6 639,681 + ahpF b0606 0.45 -1.0 639,943 + ahpF b0606 0.62 -0.3 639,943 + ahpF b0606 0.62 -0.8 639,978 + ahpF b0606 0.64 -0.1 640,001 + ahpF b0606 0.65 -0.2 640,169 - ahpF b0606 0.76 +0.9 640,169 - ahpF b0606 0.76 +0.5 640,173 - ahpF b0606 0.76 -0.1 640,314 + ahpF b0606 0.85 -0.6 640,364 + ahpF b0606 0.89 -1.3 640,391 - -0.5 640,391 - -0.1 640,447 - -0.8 640,486 + +0.0 640,486 + -0.9 640,605 + -0.2 640,605 + +0.8 640,637 - -0.2 640,637 - +0.2 640,642 - +1.2 640,647 - +0.7 640,647 - -0.9 640,670 + +0.1 640,670 + +1.6 640,782 - uspG b0607 0.28 +0.7 640,787 - uspG b0607 0.29 +1.6 640,787 - uspG b0607 0.29 -0.2 640,808 - uspG b0607 0.34 +0.2 640,813 - uspG b0607 0.35 +0.6 640,824 - uspG b0607 0.38 +0.2 640,863 - uspG b0607 0.47 +0.4 640,863 - uspG b0607 0.47 -1.7 640,887 + uspG b0607 0.52 +0.2 640,889 + uspG b0607 0.53 +0.3 640,889 + uspG b0607 0.53 -1.4 640,949 + uspG b0607 0.67 -1.2 640,949 + uspG b0607 0.67 -0.6 640,958 + uspG b0607 0.69 +0.7 640,976 + uspG b0607 0.73 -2.5 640,996 - uspG b0607 0.78 +0.3 641,001 + uspG b0607 0.79 +2.1 641,001 + uspG b0607 0.79 -1.8 641,075 + +0.5 641,080 + -0.2 641,080 + -1.2 641,080 + -0.1 641,080 + -0.2 641,088 - +0.5 641,088 - -0.8 641,139 - -0.6 641,221 - -0.9 641,221 - +0.1 641,223 + -1.2 641,266 - -0.7 641,266 - +0.4 641,286 + -0.0 641,286 + +0.0 641,342 + +0.9 641,342 + +0.6 641,372 + +1.4 641,372 + -0.2 641,434 - -0.5 641,434 - -2.0
Or see this region's nucleotide sequence