Experiment: 1/2ON soft
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mioC and asnC are separated by 89 nucleotides asnC and asnA are separated by 151 nucleotides asnA and yieM are separated by 4 nucleotides
b3742: mioC - flavodoxin (NCBI), at 3,924,035 to 3,924,478
mioC
b3743: asnC - DNA-binding transcriptional dual regulator (NCBI), at 3,924,568 to 3,925,026
asnC
b3744: asnA - asparagine synthetase AsnA (NCBI), at 3,925,178 to 3,926,170
asnA
b3745: yieM - predicted von Willibrand factor containing protein (NCBI), at 3,926,175 to 3,927,626
yieM
Position (kb)
3925
3926
3927 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3924.205 kb on + strand, within mioC at 3924.206 kb on + strand, within mioC at 3924.206 kb on + strand, within mioC at 3924.307 kb on + strand, within mioC at 3924.323 kb on - strand, within mioC at 3924.330 kb on - strand, within mioC at 3924.447 kb on - strand at 3924.458 kb on + strand at 3924.458 kb on + strand at 3924.465 kb on + strand at 3924.471 kb on - strand at 3924.507 kb on + strand at 3924.507 kb on + strand at 3924.507 kb on + strand at 3924.515 kb on + strand at 3924.515 kb on - strand at 3924.515 kb on - strand at 3924.515 kb on - strand at 3924.525 kb on - strand at 3924.530 kb on + strand at 3924.664 kb on + strand, within asnC at 3924.668 kb on - strand, within asnC at 3924.668 kb on - strand, within asnC at 3924.672 kb on - strand, within asnC at 3924.696 kb on + strand, within asnC at 3924.696 kb on + strand, within asnC at 3924.708 kb on - strand, within asnC at 3924.720 kb on - strand, within asnC at 3924.720 kb on - strand, within asnC at 3924.720 kb on - strand, within asnC at 3924.722 kb on + strand, within asnC at 3924.730 kb on - strand, within asnC at 3924.730 kb on - strand, within asnC at 3924.742 kb on - strand, within asnC at 3924.742 kb on - strand, within asnC at 3924.793 kb on - strand, within asnC at 3924.796 kb on + strand, within asnC at 3924.823 kb on - strand, within asnC at 3924.840 kb on + strand, within asnC at 3924.850 kb on - strand, within asnC at 3924.852 kb on + strand, within asnC at 3924.860 kb on - strand, within asnC at 3924.904 kb on + strand, within asnC at 3924.911 kb on + strand, within asnC at 3924.911 kb on + strand, within asnC at 3924.923 kb on - strand, within asnC at 3924.965 kb on + strand, within asnC at 3924.990 kb on - strand at 3925.206 kb on + strand at 3925.206 kb on + strand at 3925.210 kb on + strand at 3925.254 kb on + strand at 3925.301 kb on - strand, within asnA at 3925.301 kb on - strand, within asnA at 3925.301 kb on - strand, within asnA at 3925.305 kb on - strand, within asnA at 3925.312 kb on + strand, within asnA at 3925.312 kb on + strand, within asnA at 3925.312 kb on + strand, within asnA at 3925.320 kb on - strand, within asnA at 3925.320 kb on - strand, within asnA at 3925.320 kb on - strand, within asnA at 3925.348 kb on - strand, within asnA at 3925.358 kb on + strand, within asnA at 3925.388 kb on - strand, within asnA at 3925.411 kb on + strand, within asnA at 3925.417 kb on - strand, within asnA at 3925.443 kb on + strand, within asnA at 3925.443 kb on + strand, within asnA at 3925.458 kb on - strand, within asnA at 3925.462 kb on - strand, within asnA at 3925.470 kb on - strand, within asnA at 3925.470 kb on - strand, within asnA at 3925.489 kb on - strand, within asnA at 3925.520 kb on + strand, within asnA at 3925.536 kb on - strand, within asnA at 3925.543 kb on - strand, within asnA at 3925.592 kb on - strand, within asnA at 3925.592 kb on - strand, within asnA at 3925.606 kb on + strand, within asnA at 3925.607 kb on - strand, within asnA at 3925.632 kb on + strand, within asnA at 3925.632 kb on + strand, within asnA at 3925.637 kb on - strand, within asnA at 3925.644 kb on + strand, within asnA at 3925.675 kb on + strand, within asnA at 3925.675 kb on + strand, within asnA at 3925.684 kb on - strand, within asnA at 3925.686 kb on + strand, within asnA at 3925.692 kb on - strand, within asnA at 3925.693 kb on - strand, within asnA at 3925.789 kb on + strand, within asnA at 3925.797 kb on - strand, within asnA at 3925.808 kb on - strand, within asnA at 3925.808 kb on - strand, within asnA at 3925.823 kb on + strand, within asnA at 3925.842 kb on - strand, within asnA at 3925.845 kb on - strand at 3925.845 kb on - strand, within asnA at 3925.848 kb on + strand, within asnA at 3925.848 kb on + strand, within asnA at 3925.848 kb on + strand, within asnA at 3925.848 kb on + strand, within asnA at 3925.853 kb on + strand, within asnA at 3925.853 kb on + strand, within asnA at 3925.860 kb on + strand, within asnA at 3925.868 kb on + strand, within asnA at 3925.868 kb on + strand, within asnA at 3925.941 kb on + strand, within asnA at 3925.954 kb on - strand, within asnA at 3925.986 kb on - strand, within asnA at 3926.000 kb on + strand, within asnA at 3926.000 kb on + strand, within asnA at 3926.000 kb on + strand, within asnA at 3926.005 kb on + strand, within asnA at 3926.010 kb on + strand, within asnA at 3926.058 kb on + strand, within asnA at 3926.076 kb on + strand at 3926.080 kb on - strand at 3926.080 kb on - strand at 3926.093 kb on - strand at 3926.125 kb on + strand at 3926.127 kb on - strand at 3926.133 kb on - strand at 3926.135 kb on + strand at 3926.135 kb on + strand at 3926.148 kb on + strand at 3926.148 kb on + strand at 3926.165 kb on - strand at 3926.165 kb on - strand at 3926.261 kb on - strand at 3926.295 kb on + strand at 3926.308 kb on - strand at 3926.308 kb on - strand at 3926.308 kb on - strand at 3926.398 kb on + strand, within yieM at 3926.423 kb on - strand, within yieM at 3926.496 kb on + strand, within yieM at 3926.506 kb on - strand, within yieM at 3926.513 kb on + strand, within yieM at 3926.554 kb on - strand, within yieM at 3926.583 kb on + strand, within yieM at 3926.605 kb on + strand, within yieM at 3926.613 kb on - strand, within yieM at 3926.615 kb on + strand, within yieM at 3926.656 kb on + strand, within yieM at 3926.733 kb on - strand, within yieM at 3926.735 kb on - strand, within yieM at 3926.739 kb on + strand, within yieM at 3926.752 kb on - strand, within yieM at 3926.752 kb on - strand, within yieM at 3926.752 kb on - strand, within yieM at 3926.752 kb on - strand, within yieM at 3926.752 kb on - strand, within yieM at 3926.752 kb on - strand, within yieM at 3926.752 kb on - strand, within yieM at 3926.801 kb on - strand, within yieM at 3926.807 kb on + strand, within yieM at 3926.807 kb on + strand, within yieM at 3926.839 kb on - strand, within yieM at 3926.874 kb on - strand, within yieM at 3926.874 kb on - strand, within yieM at 3926.927 kb on - strand, within yieM at 3926.942 kb on + strand, within yieM at 3926.942 kb on + strand, within yieM at 3927.009 kb on + strand, within yieM at 3927.024 kb on + strand, within yieM at 3927.024 kb on + strand, within yieM at 3927.024 kb on + strand, within yieM at 3927.070 kb on + strand, within yieM at 3927.070 kb on + strand, within yieM at 3927.071 kb on + strand, within yieM at 3927.071 kb on + strand, within yieM at 3927.104 kb on - strand, within yieM at 3927.104 kb on - strand, within yieM at 3927.129 kb on + strand, within yieM at 3927.154 kb on - strand, within yieM at 3927.157 kb on + strand, within yieM at 3927.157 kb on + strand, within yieM at 3927.159 kb on + strand, within yieM at 3927.167 kb on - strand, within yieM
Per-strain Table
Position Strand Gene LocusTag Fraction 1/2ON soft remove 3,924,205 + mioC b3742 0.38 +0.4 3,924,206 + mioC b3742 0.39 -0.3 3,924,206 + mioC b3742 0.39 -0.5 3,924,307 + mioC b3742 0.61 -0.7 3,924,323 - mioC b3742 0.65 +0.1 3,924,330 - mioC b3742 0.66 +0.3 3,924,447 - -0.4 3,924,458 + -0.2 3,924,458 + -0.7 3,924,465 + +0.4 3,924,471 - -0.0 3,924,507 + +0.4 3,924,507 + +0.1 3,924,507 + -0.2 3,924,515 + +0.2 3,924,515 - -0.4 3,924,515 - +0.0 3,924,515 - +0.4 3,924,525 - +1.3 3,924,530 + -0.2 3,924,664 + asnC b3743 0.21 -0.7 3,924,668 - asnC b3743 0.22 +0.3 3,924,668 - asnC b3743 0.22 -0.4 3,924,672 - asnC b3743 0.23 -0.1 3,924,696 + asnC b3743 0.28 +0.4 3,924,696 + asnC b3743 0.28 +1.9 3,924,708 - asnC b3743 0.31 +0.5 3,924,720 - asnC b3743 0.33 +0.2 3,924,720 - asnC b3743 0.33 -0.0 3,924,720 - asnC b3743 0.33 -0.0 3,924,722 + asnC b3743 0.34 +1.0 3,924,730 - asnC b3743 0.35 -0.3 3,924,730 - asnC b3743 0.35 +0.1 3,924,742 - asnC b3743 0.38 -1.2 3,924,742 - asnC b3743 0.38 -0.3 3,924,793 - asnC b3743 0.49 -0.0 3,924,796 + asnC b3743 0.50 -0.3 3,924,823 - asnC b3743 0.56 -0.0 3,924,840 + asnC b3743 0.59 -0.2 3,924,850 - asnC b3743 0.61 +0.9 3,924,852 + asnC b3743 0.62 +0.1 3,924,860 - asnC b3743 0.64 +0.8 3,924,904 + asnC b3743 0.73 -0.0 3,924,911 + asnC b3743 0.75 +0.3 3,924,911 + asnC b3743 0.75 +0.6 3,924,923 - asnC b3743 0.77 +0.8 3,924,965 + asnC b3743 0.86 -1.1 3,924,990 - +1.1 3,925,206 + -1.1 3,925,206 + -0.1 3,925,210 + -0.2 3,925,254 + -0.2 3,925,301 - asnA b3744 0.12 +0.2 3,925,301 - asnA b3744 0.12 +0.2 3,925,301 - asnA b3744 0.12 +0.2 3,925,305 - asnA b3744 0.13 -0.4 3,925,312 + asnA b3744 0.13 -0.3 3,925,312 + asnA b3744 0.13 +0.5 3,925,312 + asnA b3744 0.13 -0.1 3,925,320 - asnA b3744 0.14 +0.5 3,925,320 - asnA b3744 0.14 +0.2 3,925,320 - asnA b3744 0.14 +1.0 3,925,348 - asnA b3744 0.17 +1.6 3,925,358 + asnA b3744 0.18 +0.2 3,925,388 - asnA b3744 0.21 -0.9 3,925,411 + asnA b3744 0.23 -2.8 3,925,417 - asnA b3744 0.24 -0.5 3,925,443 + asnA b3744 0.27 +0.2 3,925,443 + asnA b3744 0.27 -0.1 3,925,458 - asnA b3744 0.28 -0.4 3,925,462 - asnA b3744 0.29 -0.8 3,925,470 - asnA b3744 0.29 -0.6 3,925,470 - asnA b3744 0.29 +0.3 3,925,489 - asnA b3744 0.31 -0.5 3,925,520 + asnA b3744 0.34 -0.3 3,925,536 - asnA b3744 0.36 +0.4 3,925,543 - asnA b3744 0.37 +0.8 3,925,592 - asnA b3744 0.42 -0.9 3,925,592 - asnA b3744 0.42 -0.6 3,925,606 + asnA b3744 0.43 -2.0 3,925,607 - asnA b3744 0.43 +0.5 3,925,632 + asnA b3744 0.46 +0.6 3,925,632 + asnA b3744 0.46 +0.9 3,925,637 - asnA b3744 0.46 +1.5 3,925,644 + asnA b3744 0.47 -0.3 3,925,675 + asnA b3744 0.50 -0.5 3,925,675 + asnA b3744 0.50 -1.0 3,925,684 - asnA b3744 0.51 +1.1 3,925,686 + asnA b3744 0.51 +0.6 3,925,692 - asnA b3744 0.52 +1.0 3,925,693 - asnA b3744 0.52 +1.0 3,925,789 + asnA b3744 0.62 +0.7 3,925,797 - asnA b3744 0.62 -0.2 3,925,808 - asnA b3744 0.63 -0.8 3,925,808 - asnA b3744 0.63 -1.1 3,925,823 + asnA b3744 0.65 +0.5 3,925,842 - asnA b3744 0.67 +0.0 3,925,845 - -0.2 3,925,845 - asnA b3744 0.67 -2.1 3,925,848 + asnA b3744 0.67 +1.3 3,925,848 + asnA b3744 0.67 +0.2 3,925,848 + asnA b3744 0.67 +1.3 3,925,848 + asnA b3744 0.67 +0.7 3,925,853 + asnA b3744 0.68 -0.0 3,925,853 + asnA b3744 0.68 -0.2 3,925,860 + asnA b3744 0.69 +0.1 3,925,868 + asnA b3744 0.69 +0.4 3,925,868 + asnA b3744 0.69 +0.6 3,925,941 + asnA b3744 0.77 -0.3 3,925,954 - asnA b3744 0.78 -0.6 3,925,986 - asnA b3744 0.81 +0.3 3,926,000 + asnA b3744 0.83 +0.5 3,926,000 + asnA b3744 0.83 -1.2 3,926,000 + asnA b3744 0.83 +0.4 3,926,005 + asnA b3744 0.83 +0.2 3,926,010 + asnA b3744 0.84 +0.2 3,926,058 + asnA b3744 0.89 +0.6 3,926,076 + -0.0 3,926,080 - +0.8 3,926,080 - +0.7 3,926,093 - +2.0 3,926,125 + -0.3 3,926,127 - +0.1 3,926,133 - +0.0 3,926,135 + -0.1 3,926,135 + -0.2 3,926,148 + +0.5 3,926,148 + -0.7 3,926,165 - +1.0 3,926,165 - +0.3 3,926,261 - +0.9 3,926,295 + -0.1 3,926,308 - +0.9 3,926,308 - +0.1 3,926,308 - +0.4 3,926,398 + yieM b3745 0.15 +0.3 3,926,423 - yieM b3745 0.17 +0.9 3,926,496 + yieM b3745 0.22 +0.3 3,926,506 - yieM b3745 0.23 +0.4 3,926,513 + yieM b3745 0.23 +1.0 3,926,554 - yieM b3745 0.26 -0.3 3,926,583 + yieM b3745 0.28 +0.5 3,926,605 + yieM b3745 0.30 +0.8 3,926,613 - yieM b3745 0.30 +1.3 3,926,615 + yieM b3745 0.30 +0.1 3,926,656 + yieM b3745 0.33 +0.5 3,926,733 - yieM b3745 0.38 +0.1 3,926,735 - yieM b3745 0.39 +0.4 3,926,739 + yieM b3745 0.39 +1.3 3,926,752 - yieM b3745 0.40 +0.3 3,926,752 - yieM b3745 0.40 +0.5 3,926,752 - yieM b3745 0.40 +0.3 3,926,752 - yieM b3745 0.40 +0.2 3,926,752 - yieM b3745 0.40 +1.5 3,926,752 - yieM b3745 0.40 +0.3 3,926,752 - yieM b3745 0.40 +0.3 3,926,801 - yieM b3745 0.43 -0.2 3,926,807 + yieM b3745 0.44 +1.1 3,926,807 + yieM b3745 0.44 -0.7 3,926,839 - yieM b3745 0.46 +0.2 3,926,874 - yieM b3745 0.48 -0.0 3,926,874 - yieM b3745 0.48 +0.5 3,926,927 - yieM b3745 0.52 -0.1 3,926,942 + yieM b3745 0.53 +0.7 3,926,942 + yieM b3745 0.53 +1.5 3,927,009 + yieM b3745 0.57 +1.1 3,927,024 + yieM b3745 0.58 +0.4 3,927,024 + yieM b3745 0.58 +0.1 3,927,024 + yieM b3745 0.58 +0.6 3,927,070 + yieM b3745 0.62 +0.6 3,927,070 + yieM b3745 0.62 +0.6 3,927,071 + yieM b3745 0.62 +1.1 3,927,071 + yieM b3745 0.62 +0.3 3,927,104 - yieM b3745 0.64 -0.2 3,927,104 - yieM b3745 0.64 +1.3 3,927,129 + yieM b3745 0.66 +0.3 3,927,154 - yieM b3745 0.67 -0.6 3,927,157 + yieM b3745 0.68 +0.4 3,927,157 + yieM b3745 0.68 -0.1 3,927,159 + yieM b3745 0.68 +0.1 3,927,167 - yieM b3745 0.68 +0.4
Or see this region's nucleotide sequence