Experiment: 1/2ON soft
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpS and gph overlap by 8 nucleotides gph and rpe overlap by 8 nucleotides rpe and dam are separated by 17 nucleotides
b3384: trpS - tryptophanyl-tRNA synthetase (NCBI), at 3,510,656 to 3,511,660
trpS
b3385: gph - phosphoglycolate phosphatase (NCBI), at 3,511,653 to 3,512,411
gph
b3386: rpe - ribulose-phosphate 3-epimerase (NCBI), at 3,512,404 to 3,513,081
rpe
b3387: dam - DNA adenine methylase (NCBI), at 3,513,099 to 3,513,935
dam
Position (kb)
3511
3512
3513 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3511.699 kb on - strand at 3511.714 kb on + strand at 3511.714 kb on + strand at 3511.714 kb on + strand at 3511.793 kb on + strand, within gph at 3511.795 kb on + strand, within gph at 3511.820 kb on + strand, within gph at 3511.825 kb on - strand, within gph at 3511.851 kb on + strand, within gph at 3511.851 kb on + strand, within gph at 3511.856 kb on - strand, within gph at 3511.877 kb on + strand, within gph at 3511.877 kb on + strand, within gph at 3511.887 kb on + strand, within gph at 3511.897 kb on - strand, within gph at 3511.925 kb on - strand, within gph at 3511.950 kb on - strand, within gph at 3511.950 kb on - strand, within gph at 3511.965 kb on + strand, within gph at 3511.965 kb on + strand, within gph at 3511.965 kb on + strand, within gph at 3511.973 kb on - strand, within gph at 3511.975 kb on - strand, within gph at 3511.975 kb on - strand, within gph at 3511.985 kb on - strand, within gph at 3512.007 kb on + strand, within gph at 3512.011 kb on - strand, within gph at 3512.020 kb on - strand, within gph at 3512.021 kb on - strand, within gph at 3512.026 kb on - strand, within gph at 3512.030 kb on + strand, within gph at 3512.038 kb on - strand, within gph at 3512.038 kb on - strand, within gph at 3512.038 kb on - strand, within gph at 3512.104 kb on + strand, within gph at 3512.109 kb on + strand, within gph at 3512.132 kb on + strand, within gph at 3512.140 kb on - strand, within gph at 3512.140 kb on - strand, within gph at 3512.140 kb on - strand, within gph at 3512.166 kb on + strand, within gph at 3512.166 kb on + strand, within gph at 3512.172 kb on - strand, within gph at 3512.177 kb on + strand, within gph at 3512.216 kb on - strand, within gph at 3512.217 kb on + strand, within gph at 3512.218 kb on + strand, within gph at 3512.226 kb on - strand, within gph at 3512.226 kb on - strand, within gph at 3512.235 kb on + strand, within gph at 3512.246 kb on + strand, within gph at 3512.295 kb on + strand, within gph at 3512.295 kb on + strand, within gph at 3512.313 kb on - strand, within gph at 3512.313 kb on - strand, within gph at 3512.345 kb on + strand at 3512.345 kb on + strand at 3512.352 kb on + strand at 3512.358 kb on - strand at 3512.360 kb on - strand at 3512.360 kb on - strand at 3512.368 kb on - strand at 3512.425 kb on - strand at 3512.717 kb on + strand, within rpe at 3513.065 kb on + strand at 3513.065 kb on + strand at 3513.065 kb on + strand at 3513.065 kb on + strand at 3513.073 kb on - strand at 3513.122 kb on + strand at 3513.130 kb on - strand at 3513.130 kb on - strand at 3513.136 kb on + strand at 3513.146 kb on - strand at 3513.166 kb on - strand at 3513.179 kb on + strand at 3513.179 kb on + strand at 3513.180 kb on + strand at 3513.180 kb on + strand at 3513.180 kb on + strand at 3513.187 kb on - strand, within dam at 3513.187 kb on - strand, within dam at 3513.187 kb on - strand, within dam at 3513.187 kb on - strand, within dam at 3513.187 kb on - strand, within dam at 3513.187 kb on - strand, within dam at 3513.187 kb on - strand, within dam at 3513.187 kb on - strand, within dam at 3513.189 kb on + strand, within dam at 3513.189 kb on - strand, within dam at 3513.189 kb on - strand, within dam at 3513.199 kb on - strand, within dam at 3513.220 kb on + strand, within dam at 3513.220 kb on + strand, within dam at 3513.222 kb on + strand, within dam at 3513.222 kb on + strand, within dam at 3513.230 kb on - strand, within dam at 3513.232 kb on - strand, within dam at 3513.235 kb on + strand, within dam at 3513.279 kb on - strand, within dam at 3513.284 kb on + strand, within dam at 3513.286 kb on + strand, within dam at 3513.293 kb on - strand, within dam at 3513.352 kb on + strand, within dam at 3513.375 kb on - strand, within dam at 3513.377 kb on + strand, within dam at 3513.380 kb on + strand, within dam at 3513.380 kb on + strand, within dam at 3513.382 kb on + strand, within dam at 3513.396 kb on + strand, within dam at 3513.402 kb on + strand, within dam at 3513.404 kb on - strand, within dam at 3513.404 kb on - strand, within dam
Per-strain Table
Position Strand Gene LocusTag Fraction 1/2ON soft remove 3,511,699 - -0.6 3,511,714 + -0.6 3,511,714 + +0.4 3,511,714 + -1.2 3,511,793 + gph b3385 0.18 -0.3 3,511,795 + gph b3385 0.19 +0.5 3,511,820 + gph b3385 0.22 -0.2 3,511,825 - gph b3385 0.23 -0.6 3,511,851 + gph b3385 0.26 +1.6 3,511,851 + gph b3385 0.26 +1.8 3,511,856 - gph b3385 0.27 +0.4 3,511,877 + gph b3385 0.30 +0.4 3,511,877 + gph b3385 0.30 -0.6 3,511,887 + gph b3385 0.31 +0.5 3,511,897 - gph b3385 0.32 +0.5 3,511,925 - gph b3385 0.36 +0.1 3,511,950 - gph b3385 0.39 -0.3 3,511,950 - gph b3385 0.39 -0.4 3,511,965 + gph b3385 0.41 -0.4 3,511,965 + gph b3385 0.41 -0.4 3,511,965 + gph b3385 0.41 +0.4 3,511,973 - gph b3385 0.42 +0.6 3,511,975 - gph b3385 0.42 +0.5 3,511,975 - gph b3385 0.42 +0.7 3,511,985 - gph b3385 0.44 +0.8 3,512,007 + gph b3385 0.47 +0.3 3,512,011 - gph b3385 0.47 +0.9 3,512,020 - gph b3385 0.48 -0.4 3,512,021 - gph b3385 0.48 -0.5 3,512,026 - gph b3385 0.49 -0.2 3,512,030 + gph b3385 0.50 -0.1 3,512,038 - gph b3385 0.51 +0.2 3,512,038 - gph b3385 0.51 +0.9 3,512,038 - gph b3385 0.51 -0.0 3,512,104 + gph b3385 0.59 +0.9 3,512,109 + gph b3385 0.60 +0.5 3,512,132 + gph b3385 0.63 -0.2 3,512,140 - gph b3385 0.64 -0.7 3,512,140 - gph b3385 0.64 -0.3 3,512,140 - gph b3385 0.64 -0.3 3,512,166 + gph b3385 0.68 +0.2 3,512,166 + gph b3385 0.68 +0.1 3,512,172 - gph b3385 0.68 -0.2 3,512,177 + gph b3385 0.69 -0.7 3,512,216 - gph b3385 0.74 -0.3 3,512,217 + gph b3385 0.74 -0.8 3,512,218 + gph b3385 0.74 -0.0 3,512,226 - gph b3385 0.75 -0.5 3,512,226 - gph b3385 0.75 -0.2 3,512,235 + gph b3385 0.77 +1.3 3,512,246 + gph b3385 0.78 -0.1 3,512,295 + gph b3385 0.85 +0.6 3,512,295 + gph b3385 0.85 -0.4 3,512,313 - gph b3385 0.87 +0.7 3,512,313 - gph b3385 0.87 +0.6 3,512,345 + +0.2 3,512,345 + +1.5 3,512,352 + +0.4 3,512,358 - -0.4 3,512,360 - +1.0 3,512,360 - -0.0 3,512,368 - +0.1 3,512,425 - -1.7 3,512,717 + rpe b3386 0.46 -0.2 3,513,065 + -0.6 3,513,065 + +0.1 3,513,065 + -1.6 3,513,065 + +0.2 3,513,073 - -0.2 3,513,122 + +0.2 3,513,130 - +1.7 3,513,130 - -0.1 3,513,136 + -0.2 3,513,146 - -2.5 3,513,166 - -1.2 3,513,179 + +0.8 3,513,179 + -2.2 3,513,180 + +0.8 3,513,180 + -1.6 3,513,180 + -1.5 3,513,187 - dam b3387 0.11 -1.2 3,513,187 - dam b3387 0.11 -2.0 3,513,187 - dam b3387 0.11 -0.2 3,513,187 - dam b3387 0.11 -1.2 3,513,187 - dam b3387 0.11 -1.4 3,513,187 - dam b3387 0.11 -0.2 3,513,187 - dam b3387 0.11 -0.8 3,513,187 - dam b3387 0.11 -0.2 3,513,189 + dam b3387 0.11 -1.2 3,513,189 - dam b3387 0.11 -1.2 3,513,189 - dam b3387 0.11 -3.0 3,513,199 - dam b3387 0.12 +0.1 3,513,220 + dam b3387 0.14 -1.2 3,513,220 + dam b3387 0.14 -0.9 3,513,222 + dam b3387 0.15 -1.2 3,513,222 + dam b3387 0.15 -1.2 3,513,230 - dam b3387 0.16 +0.5 3,513,232 - dam b3387 0.16 -1.6 3,513,235 + dam b3387 0.16 -3.4 3,513,279 - dam b3387 0.22 -2.2 3,513,284 + dam b3387 0.22 -1.1 3,513,286 + dam b3387 0.22 -2.4 3,513,293 - dam b3387 0.23 -0.6 3,513,352 + dam b3387 0.30 -1.6 3,513,375 - dam b3387 0.33 -0.2 3,513,377 + dam b3387 0.33 -1.6 3,513,380 + dam b3387 0.34 -2.2 3,513,380 + dam b3387 0.34 -2.5 3,513,382 + dam b3387 0.34 -1.0 3,513,396 + dam b3387 0.35 -0.2 3,513,402 + dam b3387 0.36 -2.1 3,513,404 - dam b3387 0.36 -2.2 3,513,404 - dam b3387 0.36 -2.2
Or see this region's nucleotide sequence