Strain Fitness in Escherichia coli BW25113 around b3054

Experiment: 1/2ON soft

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntglnE and ygiF are separated by 22 nucleotidesygiF and htrG are separated by 241 nucleotideshtrG and cca are separated by 63 nucleotides b3053: glnE - fused deadenylyltransferase/adenylyltransferase for glutamine synthetase (NCBI), at 3,194,823 to 3,197,663 glnE b3054: ygiF - predicted adenylate cyclase (NCBI), at 3,197,686 to 3,198,987 ygiF b3055: htrG - predicted signal transduction protein (SH3 domain) (NCBI), at 3,199,229 to 3,199,849 htrG b3056: cca - fused tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase and phosphatase (NCBI), at 3,199,913 to 3,201,151 cca Position (kb) 3197 3198 3199Strain fitness (log2 ratio) -2 -1 0 1 2at 3196.922 kb on - strand, within glnEat 3196.996 kb on - strand, within glnEat 3196.997 kb on - strand, within glnEat 3196.997 kb on - strand, within glnEat 3197.014 kb on - strand, within glnEat 3197.095 kb on + strand, within glnEat 3197.196 kb on - strand, within glnEat 3197.317 kb on + strand, within glnEat 3197.317 kb on + strand, within glnEat 3197.354 kb on - strand, within glnEat 3197.354 kb on - strand, within glnEat 3197.366 kb on + strand, within glnEat 3197.369 kb on + strand, within glnEat 3197.369 kb on + strand, within glnEat 3197.397 kb on - strandat 3197.397 kb on - strandat 3197.441 kb on + strandat 3197.515 kb on + strandat 3197.532 kb on + strandat 3197.540 kb on - strandat 3197.558 kb on + strandat 3197.558 kb on + strandat 3197.558 kb on + strandat 3197.600 kb on + strandat 3197.677 kb on - strandat 3197.701 kb on + strandat 3197.701 kb on + strandat 3197.701 kb on + strandat 3197.777 kb on + strandat 3197.780 kb on - strandat 3197.782 kb on + strandat 3197.820 kb on + strand, within ygiFat 3197.834 kb on - strand, within ygiFat 3197.899 kb on - strand, within ygiFat 3197.899 kb on - strand, within ygiFat 3197.903 kb on + strand, within ygiFat 3197.999 kb on + strand, within ygiFat 3197.999 kb on + strand, within ygiFat 3198.040 kb on - strand, within ygiFat 3198.113 kb on + strand, within ygiFat 3198.113 kb on - strand, within ygiFat 3198.113 kb on - strand, within ygiFat 3198.126 kb on + strand, within ygiFat 3198.130 kb on - strand, within ygiFat 3198.151 kb on - strand, within ygiFat 3198.151 kb on - strand, within ygiFat 3198.174 kb on + strand, within ygiFat 3198.282 kb on - strand, within ygiFat 3198.321 kb on + strand, within ygiFat 3198.393 kb on - strand, within ygiFat 3198.393 kb on - strand, within ygiFat 3198.398 kb on - strand, within ygiFat 3198.398 kb on - strand, within ygiFat 3198.491 kb on + strand, within ygiFat 3198.552 kb on + strand, within ygiFat 3198.560 kb on - strand, within ygiFat 3198.561 kb on - strand, within ygiFat 3198.561 kb on - strand, within ygiFat 3198.711 kb on + strand, within ygiFat 3198.733 kb on + strand, within ygiFat 3198.733 kb on + strand, within ygiFat 3198.750 kb on + strand, within ygiFat 3198.750 kb on + strand, within ygiFat 3198.758 kb on - strand, within ygiFat 3198.824 kb on - strand, within ygiFat 3198.824 kb on - strand, within ygiFat 3198.828 kb on - strand, within ygiFat 3198.898 kb on - strandat 3198.900 kb on - strandat 3198.919 kb on + strandat 3198.991 kb on - strandat 3198.998 kb on + strandat 3198.998 kb on + strandat 3199.136 kb on + strandat 3199.139 kb on - strandat 3199.175 kb on - strandat 3199.180 kb on + strandat 3199.214 kb on + strandat 3199.274 kb on - strandat 3199.310 kb on + strand, within htrGat 3199.327 kb on - strand, within htrGat 3199.446 kb on + strand, within htrGat 3199.446 kb on + strand, within htrGat 3199.480 kb on + strand, within htrGat 3199.480 kb on + strand, within htrGat 3199.488 kb on - strand, within htrGat 3199.578 kb on - strand, within htrGat 3199.716 kb on + strand, within htrGat 3199.716 kb on + strand, within htrGat 3199.716 kb on + strand, within htrGat 3199.716 kb on + strand, within htrGat 3199.721 kb on + strand, within htrGat 3199.721 kb on + strand, within htrGat 3199.724 kb on + strand, within htrGat 3199.724 kb on + strand, within htrGat 3199.747 kb on - strand, within htrGat 3199.759 kb on - strand, within htrGat 3199.759 kb on - strand, within htrGat 3199.771 kb on - strand, within htrGat 3199.771 kb on - strand, within htrGat 3199.778 kb on + strand, within htrGat 3199.781 kb on + strand, within htrGat 3199.781 kb on + strand, within htrGat 3199.878 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1/2ON soft
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3,196,922 - glnE b3053 0.74 +0.7
3,196,996 - glnE b3053 0.76 -0.6
3,196,997 - glnE b3053 0.77 +0.9
3,196,997 - glnE b3053 0.77 +1.0
3,197,014 - glnE b3053 0.77 +0.1
3,197,095 + glnE b3053 0.80 +0.9
3,197,196 - glnE b3053 0.84 -0.3
3,197,317 + glnE b3053 0.88 -0.6
3,197,317 + glnE b3053 0.88 +0.1
3,197,354 - glnE b3053 0.89 +0.5
3,197,354 - glnE b3053 0.89 +0.9
3,197,366 + glnE b3053 0.90 -0.4
3,197,369 + glnE b3053 0.90 -1.2
3,197,369 + glnE b3053 0.90 -1.4
3,197,397 - +0.6
3,197,397 - +0.7
3,197,441 + -0.2
3,197,515 + +0.2
3,197,532 + -0.1
3,197,540 - +0.5
3,197,558 + +0.9
3,197,558 + -0.4
3,197,558 + -0.9
3,197,600 + +1.4
3,197,677 - -0.1
3,197,701 + +0.2
3,197,701 + +0.4
3,197,701 + -0.1
3,197,777 + +0.3
3,197,780 - +0.9
3,197,782 + +0.5
3,197,820 + ygiF b3054 0.10 -0.1
3,197,834 - ygiF b3054 0.11 +0.1
3,197,899 - ygiF b3054 0.16 -0.0
3,197,899 - ygiF b3054 0.16 +1.1
3,197,903 + ygiF b3054 0.17 +0.3
3,197,999 + ygiF b3054 0.24 +0.1
3,197,999 + ygiF b3054 0.24 -0.4
3,198,040 - ygiF b3054 0.27 +0.3
3,198,113 + ygiF b3054 0.33 -0.6
3,198,113 - ygiF b3054 0.33 -1.1
3,198,113 - ygiF b3054 0.33 -0.1
3,198,126 + ygiF b3054 0.34 +0.2
3,198,130 - ygiF b3054 0.34 +0.1
3,198,151 - ygiF b3054 0.36 +0.3
3,198,151 - ygiF b3054 0.36 -0.3
3,198,174 + ygiF b3054 0.37 +0.2
3,198,282 - ygiF b3054 0.46 +0.1
3,198,321 + ygiF b3054 0.49 +0.4
3,198,393 - ygiF b3054 0.54 +0.2
3,198,393 - ygiF b3054 0.54 +0.5
3,198,398 - ygiF b3054 0.55 -0.6
3,198,398 - ygiF b3054 0.55 -0.5
3,198,491 + ygiF b3054 0.62 +0.9
3,198,552 + ygiF b3054 0.67 +1.9
3,198,560 - ygiF b3054 0.67 +0.6
3,198,561 - ygiF b3054 0.67 +1.4
3,198,561 - ygiF b3054 0.67 +0.3
3,198,711 + ygiF b3054 0.79 +0.8
3,198,733 + ygiF b3054 0.80 +0.3
3,198,733 + ygiF b3054 0.80 -0.5
3,198,750 + ygiF b3054 0.82 -0.4
3,198,750 + ygiF b3054 0.82 -0.8
3,198,758 - ygiF b3054 0.82 +0.5
3,198,824 - ygiF b3054 0.87 +0.4
3,198,824 - ygiF b3054 0.87 +0.0
3,198,828 - ygiF b3054 0.88 +0.2
3,198,898 - +0.1
3,198,900 - +0.0
3,198,919 + +0.7
3,198,991 - +0.7
3,198,998 + -1.4
3,198,998 + +0.0
3,199,136 + -1.1
3,199,139 - -0.1
3,199,175 - +0.1
3,199,180 + +0.6
3,199,214 + -2.7
3,199,274 - -2.1
3,199,310 + htrG b3055 0.13 +0.4
3,199,327 - htrG b3055 0.16 -0.3
3,199,446 + htrG b3055 0.35 +1.3
3,199,446 + htrG b3055 0.35 +0.1
3,199,480 + htrG b3055 0.40 +0.4
3,199,480 + htrG b3055 0.40 +0.6
3,199,488 - htrG b3055 0.42 -0.1
3,199,578 - htrG b3055 0.56 -1.1
3,199,716 + htrG b3055 0.78 -0.1
3,199,716 + htrG b3055 0.78 +0.3
3,199,716 + htrG b3055 0.78 -0.1
3,199,716 + htrG b3055 0.78 +0.6
3,199,721 + htrG b3055 0.79 +0.2
3,199,721 + htrG b3055 0.79 +0.7
3,199,724 + htrG b3055 0.80 -0.6
3,199,724 + htrG b3055 0.80 -0.9
3,199,747 - htrG b3055 0.83 +0.4
3,199,759 - htrG b3055 0.85 +0.6
3,199,759 - htrG b3055 0.85 +0.2
3,199,771 - htrG b3055 0.87 +0.3
3,199,771 - htrG b3055 0.87 -0.7
3,199,778 + htrG b3055 0.88 -0.2
3,199,781 + htrG b3055 0.89 -0.6
3,199,781 + htrG b3055 0.89 -0.7
3,199,878 - -0.0

Or see this region's nucleotide sequence