Strain Fitness in Escherichia coli BW25113 around b2708

Experiment: 1/2ON soft

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgutM and srlR are separated by 66 nucleotidessrlR and gutQ overlap by 8 nucleotidesgutQ and ygaA overlap by 4 nucleotides b2706: gutM - DNA-binding transcriptional activator of glucitol operon (NCBI), at 2,826,643 to 2,827,002 gutM b2707: srlR - DNA-bindng transcriptional repressor (NCBI), at 2,827,069 to 2,827,842 srlR b2708: gutQ - orf, hypothetical protein (VIMSS), at 2,827,835 to 2,828,800 gutQ b2709: ygaA - putative 2-component transcriptional regulator (VIMSS), at 2,828,797 to 2,830,311 ygaA Position (kb) 2827 2828 2829Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2826.876 kb on - strand, within gutMat 2826.932 kb on + strand, within gutMat 2826.963 kb on - strand, within gutMat 2827.166 kb on - strand, within srlRat 2827.166 kb on - strand, within srlRat 2827.191 kb on - strand, within srlRat 2827.191 kb on - strand, within srlRat 2827.193 kb on + strand, within srlRat 2827.193 kb on + strand, within srlRat 2827.201 kb on - strand, within srlRat 2827.298 kb on + strand, within srlRat 2827.368 kb on + strand, within srlRat 2827.425 kb on + strand, within srlRat 2827.431 kb on + strand, within srlRat 2827.481 kb on + strand, within srlRat 2827.483 kb on - strand, within srlRat 2827.549 kb on + strand, within srlRat 2827.619 kb on + strand, within srlRat 2827.619 kb on + strand, within srlRat 2827.893 kb on - strandat 2828.144 kb on + strand, within gutQat 2828.242 kb on - strand, within gutQat 2828.313 kb on - strand, within gutQat 2828.314 kb on - strand, within gutQat 2828.394 kb on - strand, within gutQat 2828.394 kb on - strand, within gutQat 2828.394 kb on - strand, within gutQat 2828.405 kb on - strand, within gutQat 2829.156 kb on - strand, within ygaAat 2829.156 kb on - strand, within ygaAat 2829.382 kb on + strand, within ygaAat 2829.382 kb on + strand, within ygaAat 2829.400 kb on + strand, within ygaAat 2829.417 kb on + strand, within ygaAat 2829.527 kb on + strand, within ygaAat 2829.527 kb on + strand, within ygaAat 2829.544 kb on - strand, within ygaAat 2829.583 kb on + strand, within ygaAat 2829.583 kb on + strand, within ygaAat 2829.687 kb on + strand, within ygaAat 2829.687 kb on + strand, within ygaA

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Per-strain Table

Position Strand Gene LocusTag Fraction 1/2ON soft
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2,826,876 - gutM b2706 0.65 +0.3
2,826,932 + gutM b2706 0.80 +1.6
2,826,963 - gutM b2706 0.89 +0.6
2,827,166 - srlR b2707 0.13 -0.4
2,827,166 - srlR b2707 0.13 -1.4
2,827,191 - srlR b2707 0.16 +2.0
2,827,191 - srlR b2707 0.16 +0.6
2,827,193 + srlR b2707 0.16 +0.5
2,827,193 + srlR b2707 0.16 -0.6
2,827,201 - srlR b2707 0.17 -0.5
2,827,298 + srlR b2707 0.30 -0.4
2,827,368 + srlR b2707 0.39 -1.2
2,827,425 + srlR b2707 0.46 +0.5
2,827,431 + srlR b2707 0.47 +0.9
2,827,481 + srlR b2707 0.53 -0.2
2,827,483 - srlR b2707 0.53 -0.0
2,827,549 + srlR b2707 0.62 -0.9
2,827,619 + srlR b2707 0.71 -0.8
2,827,619 + srlR b2707 0.71 +0.8
2,827,893 - +1.3
2,828,144 + gutQ b2708 0.32 +0.6
2,828,242 - gutQ b2708 0.42 -0.7
2,828,313 - gutQ b2708 0.49 -0.0
2,828,314 - gutQ b2708 0.50 -0.6
2,828,394 - gutQ b2708 0.58 -0.7
2,828,394 - gutQ b2708 0.58 +1.1
2,828,394 - gutQ b2708 0.58 -0.6
2,828,405 - gutQ b2708 0.59 +0.3
2,829,156 - ygaA b2709 0.24 -0.1
2,829,156 - ygaA b2709 0.24 -0.2
2,829,382 + ygaA b2709 0.39 -0.2
2,829,382 + ygaA b2709 0.39 -2.2
2,829,400 + ygaA b2709 0.40 +2.9
2,829,417 + ygaA b2709 0.41 -0.8
2,829,527 + ygaA b2709 0.48 +1.4
2,829,527 + ygaA b2709 0.48 +0.7
2,829,544 - ygaA b2709 0.49 +0.4
2,829,583 + ygaA b2709 0.52 -0.9
2,829,583 + ygaA b2709 0.52 -0.1
2,829,687 + ygaA b2709 0.59 -0.3
2,829,687 + ygaA b2709 0.59 +0.0

Or see this region's nucleotide sequence