Experiment: 1/2ON soft
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yebZ and yobA are separated by 3 nucleotides yobA and holE are separated by 138 nucleotides holE and yobB are separated by 101 nucleotides yobB and exoX are separated by 23 nucleotides exoX and ptrB overlap by 4 nucleotides
b1840: yebZ - predicted inner membrane protein (NCBI), at 1,921,743 to 1,922,615
yebZ
b1841: yobA - hypothetical protein (NCBI), at 1,922,619 to 1,922,993
yobA
b1842: holE - DNA polymerase III, theta subunit (NCBI), at 1,923,132 to 1,923,362
holE
b1843: yobB - hypothetical protein (NCBI), at 1,923,464 to 1,924,120
yobB
b1844: exoX - exodeoxyribonuclease X (NCBI), at 1,924,144 to 1,924,806
exoX
b1845: ptrB - protease II (NCBI), at 1,924,803 to 1,926,863
ptrB
Position (kb)
1923
1924
1925 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1922.482 kb on - strand, within yebZ at 1922.528 kb on + strand at 1922.536 kb on - strand at 1922.642 kb on - strand at 1922.681 kb on + strand, within yobA at 1922.681 kb on + strand, within yobA at 1923.028 kb on + strand at 1923.059 kb on - strand at 1923.246 kb on - strand, within holE at 1923.246 kb on - strand, within holE at 1923.296 kb on + strand, within holE at 1923.364 kb on + strand at 1923.364 kb on + strand at 1923.493 kb on + strand at 1923.493 kb on + strand at 1923.666 kb on - strand, within yobB at 1923.831 kb on - strand, within yobB at 1923.840 kb on - strand, within yobB at 1923.840 kb on - strand, within yobB at 1923.846 kb on + strand, within yobB at 1923.995 kb on + strand, within yobB at 1924.003 kb on - strand, within yobB at 1924.274 kb on + strand, within exoX at 1924.274 kb on + strand, within exoX at 1924.274 kb on - strand, within exoX at 1924.309 kb on - strand, within exoX at 1924.361 kb on - strand, within exoX at 1924.361 kb on - strand, within exoX at 1924.423 kb on + strand, within exoX at 1924.423 kb on + strand, within exoX at 1924.471 kb on + strand, within exoX at 1924.507 kb on - strand, within exoX at 1924.609 kb on - strand, within exoX at 1924.715 kb on + strand, within exoX at 1924.770 kb on + strand at 1924.831 kb on - strand at 1924.893 kb on + strand at 1925.014 kb on - strand, within ptrB
Per-strain Table
Position Strand Gene LocusTag Fraction 1/2ON soft remove 1,922,482 - yebZ b1840 0.85 +1.0 1,922,528 + +0.3 1,922,536 - +1.6 1,922,642 - +0.4 1,922,681 + yobA b1841 0.17 +1.8 1,922,681 + yobA b1841 0.17 +1.0 1,923,028 + +0.0 1,923,059 - +0.5 1,923,246 - holE b1842 0.49 +0.4 1,923,246 - holE b1842 0.49 +1.6 1,923,296 + holE b1842 0.71 -1.0 1,923,364 + +1.0 1,923,364 + +0.5 1,923,493 + -0.6 1,923,493 + -0.6 1,923,666 - yobB b1843 0.31 -0.8 1,923,831 - yobB b1843 0.56 -1.3 1,923,840 - yobB b1843 0.57 +0.0 1,923,840 - yobB b1843 0.57 -1.1 1,923,846 + yobB b1843 0.58 -0.6 1,923,995 + yobB b1843 0.81 -0.2 1,924,003 - yobB b1843 0.82 -0.4 1,924,274 + exoX b1844 0.20 +0.8 1,924,274 + exoX b1844 0.20 +0.2 1,924,274 - exoX b1844 0.20 -0.6 1,924,309 - exoX b1844 0.25 +1.6 1,924,361 - exoX b1844 0.33 -2.0 1,924,361 - exoX b1844 0.33 -1.0 1,924,423 + exoX b1844 0.42 +0.8 1,924,423 + exoX b1844 0.42 +0.6 1,924,471 + exoX b1844 0.49 +0.0 1,924,507 - exoX b1844 0.55 -0.7 1,924,609 - exoX b1844 0.70 -0.3 1,924,715 + exoX b1844 0.86 +0.8 1,924,770 + -0.4 1,924,831 - +0.1 1,924,893 + +0.4 1,925,014 - ptrB b1845 0.10 -0.0
Or see this region's nucleotide sequence