Experiment: P. simiae Grant Run MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11860 and PS417_11865 are separated by 28 nucleotides PS417_11865 and PS417_11870 are separated by 149 nucleotides PS417_11870 and PS417_11875 are separated by 19 nucleotides
PS417_11860: PS417_11860 - 5-deoxy-D-glucuronate isomerase (EC 5.3.1.30) (from data) , at 2,581,946 to 2,582,734
_11860
PS417_11865: PS417_11865 - xylose isomerase, at 2,582,763 to 2,583,584
_11865
PS417_11870: PS417_11870 - 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase IolD (EC 3.7.1.22) (from data) , at 2,583,734 to 2,585,665
_11870
PS417_11875: PS417_11875 - Inositol 2-dehydrogenase (EC 1.1.1.18) (from data) , at 2,585,685 to 2,586,695
_11875
Position (kb)
2583
2584
2585
2586 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2582.776 kb on + strand at 2583.034 kb on - strand, within PS417_11865 at 2583.350 kb on + strand, within PS417_11865 at 2583.351 kb on - strand, within PS417_11865 at 2583.351 kb on - strand, within PS417_11865 at 2583.648 kb on + strand at 2583.649 kb on - strand at 2583.649 kb on - strand at 2583.720 kb on + strand at 2583.721 kb on - strand at 2583.791 kb on + strand at 2583.792 kb on - strand at 2583.792 kb on - strand at 2583.792 kb on - strand at 2583.986 kb on + strand, within PS417_11870 at 2583.987 kb on - strand, within PS417_11870 at 2584.649 kb on + strand, within PS417_11870 at 2584.811 kb on + strand, within PS417_11870 at 2585.004 kb on + strand, within PS417_11870 at 2585.005 kb on - strand, within PS417_11870 at 2585.105 kb on + strand, within PS417_11870 at 2585.106 kb on - strand, within PS417_11870 at 2585.193 kb on - strand, within PS417_11870 at 2585.216 kb on + strand, within PS417_11870 at 2585.216 kb on + strand, within PS417_11870 at 2585.216 kb on + strand, within PS417_11870 at 2585.216 kb on + strand, within PS417_11870 at 2585.290 kb on + strand, within PS417_11870 at 2585.290 kb on + strand, within PS417_11870 at 2585.333 kb on - strand, within PS417_11870 at 2585.497 kb on + strand at 2585.663 kb on + strand at 2585.663 kb on + strand at 2585.684 kb on + strand at 2586.174 kb on + strand, within PS417_11875 at 2586.174 kb on + strand, within PS417_11875 at 2586.175 kb on - strand, within PS417_11875 at 2586.249 kb on + strand, within PS417_11875 at 2586.249 kb on + strand, within PS417_11875 at 2586.249 kb on + strand, within PS417_11875 at 2586.381 kb on + strand, within PS417_11875 at 2586.522 kb on + strand, within PS417_11875 at 2586.626 kb on + strand at 2586.626 kb on + strand at 2586.627 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae Grant Run MOI 0.1 remove 2,582,776 + +1.3 2,583,034 - PS417_11865 0.33 +0.4 2,583,350 + PS417_11865 0.71 +2.2 2,583,351 - PS417_11865 0.72 -0.6 2,583,351 - PS417_11865 0.72 +0.0 2,583,648 + +0.7 2,583,649 - -1.6 2,583,649 - -0.3 2,583,720 + -0.6 2,583,721 - -0.1 2,583,791 + -0.1 2,583,792 - -2.1 2,583,792 - -0.5 2,583,792 - -2.4 2,583,986 + PS417_11870 0.13 +1.4 2,583,987 - PS417_11870 0.13 -1.1 2,584,649 + PS417_11870 0.47 +0.1 2,584,811 + PS417_11870 0.56 +1.3 2,585,004 + PS417_11870 0.66 +1.1 2,585,005 - PS417_11870 0.66 -0.3 2,585,105 + PS417_11870 0.71 +1.6 2,585,106 - PS417_11870 0.71 -0.9 2,585,193 - PS417_11870 0.76 -0.2 2,585,216 + PS417_11870 0.77 +1.3 2,585,216 + PS417_11870 0.77 +1.0 2,585,216 + PS417_11870 0.77 +1.2 2,585,216 + PS417_11870 0.77 +0.8 2,585,290 + PS417_11870 0.81 +0.6 2,585,290 + PS417_11870 0.81 +0.8 2,585,333 - PS417_11870 0.83 -0.6 2,585,497 + +0.2 2,585,663 + +0.2 2,585,663 + +0.8 2,585,684 + +0.2 2,586,174 + PS417_11875 0.48 +1.2 2,586,174 + PS417_11875 0.48 +0.4 2,586,175 - PS417_11875 0.48 +1.1 2,586,249 + PS417_11875 0.56 +0.7 2,586,249 + PS417_11875 0.56 +1.2 2,586,249 + PS417_11875 0.56 +2.1 2,586,381 + PS417_11875 0.69 +0.2 2,586,522 + PS417_11875 0.83 +0.7 2,586,626 + +1.2 2,586,626 + +0.5 2,586,627 - +1.0
Or see this region's nucleotide sequence